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11.发明授权公开(公告)号：US07482160B2
公开(公告)日：2009-01-27
申请号：US11322419
申请日：2005-12-30
申请人： Sean D. Monahan , David B. Rozema , Vladimir Trubetskoy , Paul M. Slattum , Jon A. Wolff , Vladimir G. Budker , James E. Hagstrom , Lisa J. Hanson
发明人： Sean D. Monahan , David B. Rozema , Vladimir Trubetskoy , Paul M. Slattum , Jon A. Wolff , Vladimir G. Budker , James E. Hagstrom , Lisa J. Hanson
IPC分类号： C12N15/88
CPC分类号： C12N15/87 , A61K47/59 , A61K47/60 , A61K47/645 , A61K48/00 , A61K48/0008 , A61K48/0025 , A61K48/0041
摘要： A method of forming polymers in the presence of nucleic acid using template polymerization. These methods can be used for the delivery of nucleic acids, for condensing the nucleic acid, for forming nucleic acid binding polymers, for forming supramolecular complexes containing nucleic acid and polymer, and for forming an interpolyelectrolyte complex.
摘要翻译： 使用模板聚合在核酸存在下形成聚合物的方法。 这些方法可用于递送核酸，用于冷凝核酸，用于形成核酸结合聚合物，用于形成含有核酸和聚合物的超分子复合物，并用于形成内聚电解质络合物。
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公开(公告)号：US07476401B2
公开(公告)日：2009-01-13
申请号：US11248993
申请日：2005-10-12
申请人： Sean D. Monahan , Vladimir G. Budker , Kirk Ekena , Lisa Nader
发明人： Sean D. Monahan , Vladimir G. Budker , Kirk Ekena , Lisa Nader
CPC分类号： A61K9/127 , A61K47/22 , A61K47/24 , A61K47/51 , A61K47/543 , A61K47/6949 , B82Y5/00
摘要： Compositions and methods for delivery of proteins and peptides to mammalian cells in vitro are described. Specifically, polypeptide-surfactant complexes formed from noncovalent hydrophobation of polypeptides and reversible hydrophobic modification of polypeptides are described. The compositions can be used to delivery positively charged, negatively charged and charge neutral polypeptides to cells.
摘要翻译： 描述了用于在体外将哺乳动物细胞递送蛋白质和肽的组合物和方法。 具体地，描述了由多肽的非共价疏水化和多肽的可逆疏水修饰形成的多肽 - 表面活性剂复合物。 该组合物可用于将带正电荷的带负电荷的中性多肽递送至细胞。
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公开(公告)号：US07138382B2
公开(公告)日：2006-11-21
申请号：US10619778
申请日：2003-07-15
IPC分类号： A61K48/00 , A61K31/711 , A61K31/7105 , A61K38/04 , C12N15/87 , A61K9/127
CPC分类号： C12N15/87 , A61K38/00 , A61K48/00 , A61K48/0008 , A61K48/0041 , C07H21/00 , C07H21/04 , C07K7/06
摘要： A system relating to the delivery of desired compounds (e.g., drugs and nucleic acids) into cells using pH-sensitive delivery systems. The system provides compositions and methods for the delivery and release of a compound to a cell.
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公开(公告)号：US07049142B1
公开(公告)日：2006-05-23
申请号：US09631152
申请日：2000-08-02
CPC分类号： A61K48/0008 , A61K48/00 , C12N15/87
摘要： A process and compound wherein nucleic acids can be modified with a host of molecules and maintain their ability to be expressed. A modifying chemical attachment of polyions to polynucleotides can be used to facilitate the change of tertiary structure of the nucleic acid and in some cases condensation of nucleic acids into smaller, charged particles useful in delivering the nucleic acid to a cell.
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公开(公告)号：US06936729B2
公开(公告)日：2005-08-30
申请号：US09795607
申请日：2001-02-28
IPC分类号： A61K48/00 , C07C323/60 , C07C323/62 , C07D207/46 , C11D1/60 , A61K9/127 , C12N15/88
CPC分类号： C07D207/46 , A61K48/00 , C07C323/60 , C07C323/62
摘要： A labile disulfide-containing compound under physiological conditions, comprising: the disulfide-containing compound having a labile disulfide bond that is either a disulfide bond that is cleaved more rapidly than oxidized glutathione or a disulfide bond constructed from thiols in which one of the constituent thiols has a lower pKa than glutathione or a disulfide bond that is activated by intramolecular attack from a free thiol.
摘要翻译： 在生理条件下的不稳定二硫化物化合物，其包含：具有不稳定二硫键的二硫键化合物，其是比氧化型谷胱甘肽更快地裂解的二硫键或由其中一种组成硫醇组成的硫醇构成的二硫键 具有比谷胱甘肽低的pKa或通过游离硫醇的分子内攻击而活化的二硫键。
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公开(公告)号：US06673612B2
公开(公告)日：2004-01-06
申请号：US10081461
申请日：2002-02-21
IPC分类号： C12N1588
CPC分类号： A61K48/0025 , A61K9/1075
摘要： A complex is described that is deliverable to a cell comprising inserting a nucleic acid or other cargo into a reverse micelle. The reverse micelle has the property to compact the nucleic acid for easier delivery.
摘要翻译： 描述了可递送至细胞的复合物，包括将核酸或其他货物插入反胶束中。 反胶束具有压实核酸以便于递送的性质。
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公开(公告)号：US06429200B1
公开(公告)日：2002-08-06
申请号：US09354957
申请日：1999-07-16
IPC分类号： A61K317105
CPC分类号： A61K9/1075 , A61K48/0025
摘要： A complex is described for delivery to a cell comprising inserting a nucleic acid into a reverse micelle. The reverse micelle has the property to compact the nucleic acid for easier delivery. Other molecules are used to interact with the nucleic acid—micelle complex to further enhance delivery such as a surfactant having a disulfide bond.
摘要翻译： 描述了一种复合物用于递送至包含将核酸插入反胶束中的细胞。 反胶束具有压实核酸以便于递送的性质。 使用其他分子与核酸 - 胶束复合物相互作用以进一步增强递送，例如具有二硫键的表面活性剂。
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18.发明授权Process of transfecting a cell with a polynucleotide mixed with an amphipathic compound and a DNA-binding protein 失效用与两亲化合物和DNA结合蛋白质混合的多核苷酸转染细胞的方法公开(公告)号：US06180784B2
公开(公告)日：2001-01-30
申请号：US09020566
申请日：1998-01-17
IPC分类号： C07D25502
CPC分类号： C12N15/87
摘要： The present invention provides a process of transfecting a cell with a polynucleotide mixed with one or more amphipathic compounds and an effective amount of a DNA-binding protein. Exemplary and preferred DNA-binding proteins are H1, H2A, and H2B. Exemplary and preferred amphipathic compounds are cationic amphipathic compounds.
摘要翻译： 本发明提供了用与一种或多种两亲化合物和有效量的DNA结合蛋白混合的多核苷酸转染细胞的方法。 示例性和优选的DNA结合蛋白是H1，H2A和H2B。 示例性和优选的两亲性化合物是阳离子两亲性化合物。
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公开(公告)号：US07507722B1
公开(公告)日：2009-03-24
申请号：US09707000
申请日：2000-11-06
申请人： Jon A. Wolff , Vladimir G. Budker
发明人： Jon A. Wolff , Vladimir G. Budker
IPC分类号： A01K43/04 , A61K31/715
CPC分类号： A61K31/715 , C12N15/89
摘要： Disclosed is a process for transfecting genetic material into a mammalian cell to alter endogenous properties of the cell. The process comprises designing a polynucleotide for transfection. Then the polynucleotide is inserted into a mammalian blood vessel. Prior to insertion, subsequent to insertion, or concurrent with insertion the permeability of the vessel is increased using an exterior cuff thereby the genetic material is delivered to the parenchymal cell.
摘要翻译： 公开了将遗传物质转染入哺乳动物细胞以改变细胞的内源性质的方法。 该方法包括设计用于转染的多核苷酸。 然后将多核苷酸插入哺乳动物血管。 在插入之前，插入之后或插入同时，使用外部袖带增加血管的渗透性，从而将遗传物质递送至实质细胞。
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公开(公告)号：US07491538B2
公开(公告)日：2009-02-17
申请号：US11189321
申请日：2005-07-26
IPC分类号： C12N15/88
摘要： A process and compound wherein nucleic acids can be modified with a host of molecules and maintain their ability to be expressed. A modifying chemical attachment of polyions to polynucleotides can be used to facilitate the change of tertiary structure of the nucleic acid and in some cases condensation of nucleic acids into smaller, charged particles useful in delivering the nucleic acid to a cell.
摘要翻译： 一种方法和化合物，其中核酸可以用多种分子修饰并保持其表达能力。 可以将聚阴离子与多核苷酸的修饰化学连接用于促进核酸的三级结构的改变，并且在一些情况下，将核酸缩合成用于将核酸递送至细胞的较小带电粒子。
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