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1.发明授权Process of transfecting a cell with a polynucleotide mixed with an amphipathic compound and a DNA-binding protein 失效用与两亲化合物和DNA结合蛋白质混合的多核苷酸转染细胞的方法公开(公告)号：US06180784B2
公开(公告)日：2001-01-30
申请号：US09020566
申请日：1998-01-17
IPC分类号： C07D25502
CPC分类号： C12N15/87
摘要： The present invention provides a process of transfecting a cell with a polynucleotide mixed with one or more amphipathic compounds and an effective amount of a DNA-binding protein. Exemplary and preferred DNA-binding proteins are H1, H2A, and H2B. Exemplary and preferred amphipathic compounds are cationic amphipathic compounds.
摘要翻译： 本发明提供了用与一种或多种两亲化合物和有效量的DNA结合蛋白混合的多核苷酸转染细胞的方法。 示例性和优选的DNA结合蛋白是H1，H2A和H2B。 示例性和优选的两亲性化合物是阳离子两亲性化合物。
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公开(公告)号：US07473419B2
公开(公告)日：2009-01-06
申请号：US11268276
申请日：2005-11-07
CPC分类号： A61K48/0041 , A61K38/00 , A61K48/00 , A61K48/0008 , A61K48/0075 , A61K48/0083 , C12N15/87
摘要： Disclosed is a process for providing for expression of an exogenous nucleic acid in an extravascular parenchymal cell of a mammal. The nucleic acid is inserted into a vessel of a mammal and the permeability of the vessel is increased. Increasing permeability of the vessel allows delivery of the nucleic acid to an extravascular parenchymal cell.
摘要翻译： 公开了一种提供外源核酸在哺乳动物血管外实质细胞中表达的方法。 将核酸插入哺乳动物的容器中并增加血管的渗透性。 增加容器的渗透性允许将核酸递送至血管外实质细胞。
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3.发明授权Devices and processes for distribution of genetic material to mammalian limb 失效将遗传物质分配给哺乳动物肢体的装置和方法公开(公告)号：US07214369B2
公开(公告)日：2007-05-08
申请号：US10429478
申请日：2003-05-05
CPC分类号： A61K48/0075 , A61K38/1825 , A61K38/1866
摘要： A process is described for the delivery of a therapeutic polynucleotide to limb muscle tissue suffering from or potentially suffering from ischemia. The polynucleotide is inserted into a mammalian limb vessel such as an artery. Delivery efficiency and distribution is enhanced by combining injection of a solution containing the polynucleotide with the use of an externally applied cuff.
摘要翻译： 描述了将治疗性多核苷酸递送至患有或可能患有缺血的肢体肌肉组织的方法。 将多核苷酸插入哺乳动物肢体血管如动脉。 通过将含有多核苷酸的溶液的注入与使用外部应用的袖带组合来提高递送效率和分布。
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公开(公告)号：US07091041B2
公开(公告)日：2006-08-15
申请号：US10627247
申请日：2003-07-25
CPC分类号： A61K48/0025 , A61K9/1075
摘要： A complex is described that is deliverable to a cell comprising inserting a nucleic acid or other cargo into a reverse micelle. The reverse micelle has the property to compact the nucleic acid for easier delivery.
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公开(公告)号：US07015040B2
公开(公告)日：2006-03-21
申请号：US11008856
申请日：2004-12-10
IPC分类号： C12N15/88
CPC分类号： A61K48/0041 , A61K38/00 , A61K48/00 , A61K48/0008 , A61K48/0075 , A61K48/0083 , C12N15/87
摘要： Disclosed is a process for providing for expression of an exogenous nucleic acid in an extravascular parenchymal cell of a mammal. The nucleic acid is inserted into a vessel of a mammal and the permeability of the vessel is increased. Increasing permeability of the vessel allows delivery of the nucleic acid to an extravascular parenchymal cell.
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公开(公告)号：US06897068B2
公开(公告)日：2005-05-24
申请号：US10085378
申请日：2002-02-27
申请人： Sean D. Monahan , Jon A. Wolff , James E. Hagstrom , Vladimir G. Budker , DAvid B. Rozema , Paul M. Slattum
发明人： Sean D. Monahan , Jon A. Wolff , James E. Hagstrom , Vladimir G. Budker , DAvid B. Rozema , Paul M. Slattum
CPC分类号： A61K48/0008 , A61K48/0041 , A61K48/0083
摘要： Disclosed is a complex for providing nucleic acid expression in a cell. A polynucleotide and a polymer are mixed together to form the complex wherein the zeta potential of the complex is not positive. Then the complex is delivered to the cell wherein the nucleic acid is expressed.
摘要翻译： 公开了用于在细胞中提供核酸表达的复合物。 将多核苷酸和聚合物混合在一起形成复合物，其中络合物的ζ电位不是正的。 然后将复合物递送到其中表达核酸的细胞。
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公开(公告)号：US06881576B2
公开(公告)日：2005-04-19
申请号：US10095752
申请日：2002-03-11
CPC分类号： C12N15/88 , A61K47/58 , A61K47/59 , A61K47/60 , A61K47/645 , A61K48/00 , A61K48/0008 , A61K48/0025 , A61K48/0041 , C12N15/87
摘要： A polyampholyte is utilized in a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The complex can be formed with an appropriate amount of positive and/or negative charge such that the resulting complex can be delivered to the extravascular space and may be further delivered to a cell.
摘要翻译： 为了将核酸递送至细胞，聚阴离子用于缩合的多核苷酸复合物。 络合物可以用适量的正电荷和/或负电荷形成，使得所得复合物可以递送到血管外空间并且可以进一步递送至细胞。
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公开(公告)号：US06867196B1
公开(公告)日：2005-03-15
申请号：US09330909
申请日：1999-06-11
申请人： Jon A. Wolff , Hans Herweijer , Larry F. Whitesell , Matthew R. Wolff , Sean D. Monahan , Paul M. Slattum , James E. Hagstrom , Vladimir G. Budker , David B. Rozema
发明人： Jon A. Wolff , Hans Herweijer , Larry F. Whitesell , Matthew R. Wolff , Sean D. Monahan , Paul M. Slattum , James E. Hagstrom , Vladimir G. Budker , David B. Rozema
CPC分类号： A61K48/0008 , A61K48/0075
摘要： A process for delivering a nucleic acid to a cardiac tissue cell in a mammal is described, comprising introducing a composition consisting of a nucleic acid to a blood vessel, which subsequently delivers the nucleic acid to the cardiac tissue cell. The nucleic acid can be DNA or RNA or plasmid DNA or viral. This process is for purposes of gene therapy, and research.
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9.发明授权公开(公告)号：US06706922B2
公开(公告)日：2004-03-16
申请号：US09993216
申请日：2001-11-16
申请人： Jon A. Wolff , James E. Hagstrom , Vladimir G. Budker , Vladimir S. Trubetskoy , Paul M. Slattum , Lisa J. Hanson
发明人： Jon A. Wolff , James E. Hagstrom , Vladimir G. Budker , Vladimir S. Trubetskoy , Paul M. Slattum , Lisa J. Hanson
IPC分类号： C07C21163
CPC分类号： C12N15/87 , A61K47/59 , A61K47/60 , A61K47/645 , A61K48/00 , A61K48/0008 , A61K48/0025 , A61K48/0041
摘要： A method of forming polymers in the presence of nucleic acid using template polymerization. Also, a method of having the polymerization occur in heterophase systems. These methods can be used for the delivery of nucleic acids, for condensing the nucleic acid, for forming nucleic acid binding polymers, for forming supramolecular complexes containing nucleic acid and polymer, and for forming an interpolyelectrolyte complex.
摘要翻译： 使用模板聚合在核酸存在下形成聚合物的方法。 此外，在异相体系中发生聚合的方法。 这些方法可用于递送核酸，用于冷凝核酸，用于形成核酸结合聚合物，用于形成含有核酸和聚合物的超分子复合物，并用于形成内聚电解质络合物。
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公开(公告)号：US06627616B2
公开(公告)日：2003-09-30
申请号：US09447966
申请日：1999-11-23
申请人： Sean D. Monahan , Jon A. Wolff , Paul M. Slattum , James E. Hagstrom , Vladimir G. Budker , David B. Rozema
发明人： Sean D. Monahan , Jon A. Wolff , Paul M. Slattum , James E. Hagstrom , Vladimir G. Budker , David B. Rozema
IPC分类号： C12N1500
CPC分类号： C12N15/113 , A61K31/70 , A61K47/58 , A61K47/59 , A61K47/593 , A61K47/62 , A61K47/6455 , A61K48/00 , A61K48/0008 , A61K48/0041 , A61K48/0083 , C07H21/00 , C12N15/87 , C12N2320/32 , A61K2300/00
摘要： Disclosed is a process for transfecting genetic material into a mammalian cell to alter endogenous properties of the cell. The process comprises designing a polynucleotide for transfection. Then the polynucleotide is inserted into a mammalian vessel such as a tail vein or artery. Prior to insertion, subsequent to insertion, or concurrent with insertion the permeability of the vessel is increased thereby the genetic material is delivered to the parenchymal cell altering endogenous properties of the cell.
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