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22 条记录 (耗时 0.015 秒)

    Polycationic buffers and method for gel electrophoresis of nucleic acids
    11.
    发明授权
    Polycationic buffers and method for gel electrophoresis of nucleic acids 失效
    聚阳离子缓冲液和核酸凝胶电泳方法

    公开(公告)号：US4936963A

    公开(公告)日：1990-06-26

    申请号：US356590

    申请日：1989-05-23

    申请人： Wlodek Mandecki ,  Mark A. Hayden

    发明人： Wlodek Mandecki ,  Mark A. Hayden

    IPC分类号： G01N27/447

    CPC分类号： G01N27/44747

    摘要： The present invention provides for polycationic buffers for the gel electrophoresis of and methods for obtaining improved separation and resolution of nucleic acids. In particular, the invention provides for a buffer consisting essentially of histidine for the gel electrophoresis of oligonucleotides ranging from a few to about 70 nucleotides in length. Polycationic buffers may be used for both analytical and preparative purposes and result in a nearly two-fold improvement in both separation and resolution of the nucleic acid bands. Furthermore, the use of polycationic buffers reduces the electrophoresis time by a factor of about three due to the low conductivity of the buffers.

    摘要翻译： 本发明提供了用于凝胶电泳的聚阳离子缓冲液和获得改进的核酸分离和分离的方法。 特别地，本发明提供基本上由组氨酸组成的缓冲液，用于长度为几个至约70个核苷酸的寡核苷酸的凝胶电泳。 聚阳离子缓冲液可以用于分析和制备目的，并且导致核酸条带的分离和分离两者的改善近两倍。 此外，由于缓冲液的低电导率，使用聚阳离子缓冲液将电泳时间减少约3倍。

    Apparatus and method to deliver a microchip
    12.
    发明授权
    Apparatus and method to deliver a microchip 有权
    传送微芯片的装置和方法

    公开(公告)号：US08353917B2

    公开(公告)日：2013-01-15

    申请号：US12889007

    申请日：2010-09-23

    申请人： Wlodek Mandecki ,  Hanan Davidowitz ,  Maryann Gruda ,  Edward Kuspiel ,  Joseph Nolfo ,  Ziye (Jay) Qian ,  Gary H. Reitnauer

    发明人： Wlodek Mandecki ,  Hanan Davidowitz ,  Maryann Gruda ,  Edward Kuspiel ,  Joseph Nolfo ,  Ziye (Jay) Qian ,  Gary H. Reitnauer

    IPC分类号： A61B17/00

    CPC分类号： A61B17/3468 ,  A61B90/90 ,  A61B90/98 ,  A61D1/00

    摘要： Provided are methods and apparatus including an injector system adapted for injecting a microchip comprising: an inserter comprising a rod or tube having a proximal and distal end and having a point at the distal end that defines a skin-piercing side of the inserter; a means for engaging the microchip near the distal end of the inserter, but removed in the proximal direction from the distal tip of the inserter, which means prevents rotation of an engaged microchip in two or three axes of rotation; and means for releasing the microchip from the inserter once the inserter has been inserted into a perforatable substrate.

    摘要翻译： 提供了包括适于注射微芯片的注射器系统的方法和装置，包括：插入器，其包括具有近端和远端的杆或管，并且在远端具有限定插入器的穿刺侧的点; 用于在插入器的远端附近接合微芯片的装置，但是在近端方向上从插入器的远端去除，这意味着防止接合的微芯片在两个或三个旋转轴线中旋转; 以及一旦插入器已经插入到可穿孔的基底中，则从插入器释放微芯片的装置。

    Fluorescence methods of detecting a protein-elongation related complex and corollary sequence information
    13.
    发明授权
    Fluorescence methods of detecting a protein-elongation related complex and corollary sequence information 有权
    检测蛋白质延伸相关复合物的荧光方法和推论序列信息

    公开(公告)号：US08323886B2

    公开(公告)日：2012-12-04

    申请号：US10871260

    申请日：2004-06-18

    申请人： Wlodek Mandecki

    发明人： Wlodek Mandecki

    IPC分类号： C12Q1/68

    CPC分类号： G01N33/583 ,  G01N33/542

    摘要： Provided is an assay method for acquiring sequence information about a single ribonucleic acid, the method comprising: (A) contacting components including a ribosomal particle comprising a ribosome and the ribonucleic acid with a complex comprising moieties that are aa1-tRNA, EF-Tu and GTP or a functional analog, wherein at least one said moiety is labeled with a fluorescent molecule, wherein aa1-tRNA is a tRNA for first amino acid aa1; (B) providing, in conjunction with the contacted components, a ribosomal translation component mixture; (C) generating a fluorescent signal from a labeled moiety in correlation with the incorporation of aa1 into protein and the labeled moiety is thereby separated from the complex; and (D) detecting the signal to provide sequence information about the ribonucleic acid.

    摘要翻译： 提供了一种用于获取关于单个核糖核酸的序列信息的测定方法，所述方法包括：（A）将包含核糖体和核糖核酸的核糖体颗粒的组分与包含aa1-tRNA，EF-Tu和 GTP或功能类似物，其中至少一个所述部分用荧光分子标记，其中aa1-tRNA是第一氨基酸aa1的tRNA; （B）与所接触的组分结合提供核糖体翻译组分混合物; （C）与aa1掺入蛋白质相关的从标记部分产生荧光信号，从而将该标记部分与复合物分离; 和（D）检测信号以提供关于核糖核酸的序列信息。

    Multiplex assay for nucleic acids employing transponders
    14.
    发明授权
    Multiplex assay for nucleic acids employing transponders 失效
    使用转发器的核酸的多重测定

    公开(公告)号：US06361950B1

    公开(公告)日：2002-03-26

    申请号：US09541744

    申请日：2000-04-03

    申请人： Wlodek Mandecki

    发明人： Wlodek Mandecki

    IPC分类号： C12Q168

    CPC分类号： G01N33/54373 ,  B01J2219/00567 ,  C12Q1/6825 ,  C12Q1/6834 ,  C12Q1/6837 ,  C40B70/00 ,  G01N15/14 ,  G01N33/54313 ,  C12Q2565/607

    摘要： Disclosed are materials and methods for performing multiplex assays for nucleic acids, in which a transponder is associated with the bead(s) forming the solid phase used in the assay, nucleic acid probes are bound to the surface of the particles, and data concerning the assay is encoded on the transponder. A dedicated read/write device is used to remotely encode or read the data.

    摘要翻译： 公开了用于对核酸进行多重测定的材料和方法，其中应答器与形成测定中使用的固相的珠相关联，核酸探针结合到颗粒的表面，并且关于 测定法在转发器上编码。 专用的读/写设备用于远程编码或读取数据。

    Metal nanoparticle structures for enhancing fluorescence-based assays
    17.
    发明授权
    Metal nanoparticle structures for enhancing fluorescence-based assays 有权
    用于增强基于荧光的测定的金属纳米颗粒结构

    公开(公告)号：US08785352B2

    公开(公告)日：2014-07-22

    申请号：US13643875

    申请日：2011-04-29

    申请人： Wlodek Mandecki ,  Ji Li ,  Zhuying Wang

    发明人： Wlodek Mandecki ,  Ji Li ,  Zhuying Wang

    IPC分类号： C40B20/04 ,  G01N21/64 ,  C40B40/06 ,  C40B40/10

    CPC分类号： G01N21/6486 ,  G01N33/587 ,  Y10T428/265

    摘要： Provided, among other things, is a multiplex assay comprising: conducting a fluorescence-developing assay on microtabs having at least one surface that shows plasmonic enhancement, wherein a plurality of the microtabs have unique probes affixed to their plasmonically enhanced surfaces; and measuring the fluorescence associated with the substrates and identifying the correlated probe by for the microtab. The microtabs can be, for example, MTPs that send a unique identifier, and the correlated probe can be identified by querying the MTPs for their identifier.

    摘要翻译： 除其他之外，提供多重测定，其包括：对具有至少一个表现出等离子体激增的表面的微切片进行荧光显影分析，其中多个微切片具有固定于其等离子体增强表面的独特探针; 并测量与底物相关的荧光，并通过microtab鉴定相关探针。 微型计算机可以是例如发送唯一标识符的MTP，并且可以通过查询MTP的标识符来识别相关探测器。

    APPARATUS AND METHOD TO DELIVER A MICROCHIP
    18.
    发明申请
    APPARATUS AND METHOD TO DELIVER A MICROCHIP 有权
    提供微型计算机的装置和方法

    公开(公告)号：US20110077659A1

    公开(公告)日：2011-03-31

    申请号：US12889007

    申请日：2010-09-23

    申请人： Wlodek Mandecki ,  Hanan Davidowitz ,  Maryann Gruda ,  Edward Kuspiel ,  Joseph Nolfo ,  Ziye "Jay" Qian ,  Gary H. Reitnauer

    发明人： Wlodek Mandecki ,  Hanan Davidowitz ,  Maryann Gruda ,  Edward Kuspiel ,  Joseph Nolfo ,  Ziye "Jay" Qian ,  Gary H. Reitnauer

    IPC分类号： A61B17/00 ,  A61D1/00

    CPC分类号： A61B17/3468 ,  A61B90/90 ,  A61B90/98 ,  A61D1/00

    摘要： Provided are methods and apparatus including an injector system adapted for injecting a microchip comprising: an inserter comprising a rod or tube having a proximal and distal end and having a point at the distal end that defines a skin-piercing side of the inserter; a means for engaging the microchip near the distal end of the inserter, but removed in the proximal direction from the distal tip of the inserter, which means prevents rotation of an engaged microchip in two or three axes of rotation; and means for releasing the microchip from the inserter once the inserter has been inserted into a perforatable substrate.

    摘要翻译： 提供了包括适于注射微芯片的注射器系统的方法和装置，包括：插入器，其包括具有近端和远端的杆或管，并且在远端具有限定插入器的穿刺侧的点; 用于在插入器的远端附近接合微芯片的装置，但是在近端方向上从插入器的远端去除，这意味着防止接合的微芯片在两个或三个旋转轴线中旋转; 以及一旦插入器已经插入到可穿孔的基底中，则从插入器释放微芯片的装置。