公开(公告)号：US09313558B1

公开(公告)日：2016-04-12

申请号：US13239984

申请日：2011-09-22

申请人： Wlodek Mandecki ,  Ziye Qian ,  Richard Morris ,  Garry Thomas Jolley-Rogers ,  David Keith Yeates ,  Philip John Suter ,  James Reginald Croft ,  Edith Margaret Cawsey ,  Jeffrey Michael Webb

发明人： Wlodek Mandecki ,  Ziye Qian ,  Richard Morris ,  Garry Thomas Jolley-Rogers ,  David Keith Yeates ,  Philip John Suter ,  James Reginald Croft ,  Edith Margaret Cawsey ,  Jeffrey Michael Webb

IPC分类号： H04Q5/22 ,  G08B13/14

CPC分类号： H04Q5/22 ,  H04Q2213/13095

摘要： Provided herein, among other things, is an assembly comprising a pin or a piece of jewelry and a transponder affixed thereto. The transponder can be a very small, light-triggered transponder (“MTP”).

摘要翻译： 这里提供了一种组件，其包括销或一件首饰和附接到其上的应答器。 应答器可以是非常小的，轻触发的应答器（“MTP”）。

公开(公告)号：US6001571A

公开(公告)日：1999-12-14

申请号：US858363

申请日：1997-05-19

申请人： Wlodek Mandecki

发明人： Wlodek Mandecki

IPC分类号： C12Q1/68 ,  C40B70/00 ,  G01N15/14 ,  G01N33/543 ,  C12P19/34

CPC分类号： G01N33/54373 ,  C12Q1/6825 ,  C12Q1/6834 ,  C12Q1/6837 ,  G01N33/54313 ,  B01J2219/00567 ,  C40B70/00 ,  G01N15/14

摘要： Disclosed are materials and methods for performing multiplex assays for nucleic acids, in which a transponder is associated with the bead(s) forming the solid phase used in the assay, nucleic acid probes are bound to the surface of the particles, and data concerning the assay is encoded on the transponder. A dedicated read/write device is used to remotely encode or read the data.

公开(公告)号：US5981166A

公开(公告)日：1999-11-09

申请号：US842383

申请日：1997-04-23

申请人： Wlodek Mandecki

发明人： Wlodek Mandecki

IPC分类号： B01J19/00 ,  C40B70/00 ,  G01N33/551 ,  C12Q1/00 ,  C12Q1/18 ,  C12Q1/42 ,  G01N33/53

CPC分类号： G01N33/551 ,  B01J19/0046 ,  B01J2219/00567 ,  B01J2219/00702 ,  C40B70/00 ,  Y10S435/968

摘要： The invention provides a method to rapidly screen chemical compounds by delivering the compounds to the assay as a coating on transponders, rather than as powder or solution. The transponder's function is to store data that identify the compound. The data can be decoded in any moment of the assay, and the identity of the desired compound established.

摘要翻译： 本发明提供了一种通过将化合物作为转发器上的涂层而不是粉末或溶液将化合物递送到测定中来快速筛选化合物的方法。 应答器的功能是存储识别化合物的数据。 可以在测定的任何时刻对数据进行解码，并建立所需化合物的身份。

公开(公告)号：US20180085747A1

公开(公告)日：2018-03-29

申请号：US13239779

申请日：2011-09-22

申请人： Wlodek Mandecki ,  Maryann Gruda ,  Ziye Qian ,  Efrain Rodriguez

发明人： Wlodek Mandecki ,  Maryann Gruda ,  Ziye Qian ,  Efrain Rodriguez

IPC分类号： C12M3/00 ,  B29C65/44 ,  G06K19/00 ,  G06K7/00

CPC分类号： B01L3/508 ,  B01L3/5453 ,  B01L2300/0851 ,  G01N1/42 ,  G01N35/00732 ,  G01N2035/00772 ,  G01N2035/0406 ,  G08B21/18

摘要： Provided herewith, among other things, is an assembly comprising a test tube with an affixed very small, light-triggered transponder (“MTP”).

公开(公告)号：US06440700B1

公开(公告)日：2002-08-27

申请号：US09640340

申请日：2000-08-16

申请人： Wlodek Mandecki

发明人： Wlodek Mandecki

IPC分类号： C12P2106

CPC分类号： C40B40/02 ,  C12N15/1037 ,  C12N15/64 ,  C12N15/70 ,  C12P21/02

摘要： Disclosed are materials and methods for practicing combinatorial protein synthesis based on ribosomal frameshifting. The genes, encoding the proteins to be synthesized, are constructed by assembly of several double-stranded DNA fragments and are cloned into bacteria. The genes include interspersed frameshifting sequences. Proteins are made in the cell by translating nucleotide sequences in a combinatorial mode. The invention can be used for the selection of new, modified or improved proteins.

摘要翻译： 公开了基于核糖体移码进行组合蛋白质合成的材料和方法。 编码待合成蛋白质的基因是通过组装几条双链DNA片段构建的，并被克隆到细菌中。 基因包括散置的移码序列。 通过以组合模式翻译核苷酸序列在细胞中制备蛋白质。 本发明可用于选择新的，修饰的或改进的蛋白质。

公开(公告)号：US06376187B1

公开(公告)日：2002-04-23

申请号：US09614076

申请日：2000-07-11

申请人： Wlodek Mandecki

发明人： Wlodek Mandecki

IPC分类号： G01N33543

CPC分类号： C12Q1/6825 ,  B01J2219/00567 ,  C12Q1/6834 ,  C40B70/00 ,  G01N15/14 ,  G01N33/54313 ,  G01N33/54373 ,  Y10S435/81 ,  Y10S435/973 ,  Y10S435/975 ,  Y10S436/805 ,  Y10S436/806 ,  Y10S436/808 ,  Y10S436/824

摘要： Disclosed are materials and methods for detecting biomolecules in samples employing transponders associated with the bead(s) used as the solid phase in the assay, and information pertinent to the assay is encoded on the transponders memory elements. A dedicated read/write device is used remotely to encode or remotely to read the information. The invention can be used in direct or competitive ELISA-type assays, or in multiplex assays for the simultaneous assay of several analytes.

摘要翻译： 公开了用于在样品中检测生物分子的材料和方法，所述样品使用与测定中用作固相的珠相关联的转发器，并且与转移体记忆元件编码与测定相关的信息。 专用的读/写设备被远程用于编码或远程读取信息。 本发明可用于直接或竞争性ELISA型测定，或用于多种分析物的同时测定的多重测定中。

公开(公告)号：US5736332A

公开(公告)日：1998-04-07

申请号：US564860

申请日：1995-11-30

申请人： Wlodek Mandecki

发明人： Wlodek Mandecki

IPC分类号： G01N33/50 ,  B01J19/00 ,  C12N15/09 ,  C12Q1/68 ,  C40B40/06 ,  C40B70/00 ,  C07H21/04 ,  C12P19/34 ,  G01N33/00

CPC分类号： C12Q1/6874 ,  B01J19/0046 ,  C12Q1/6869 ,  B01J2219/005 ,  B01J2219/00567 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00722 ,  C40B40/06 ,  C40B70/00 ,  Y10S435/81 ,  Y10S435/973 ,  Y10T436/143333

摘要： A method is described for determining the sequence of nucleic acids. The method employs small solid phase particles having transponders, with a primary layer of an oligonucleotide of known sequence attached to the outer surface of the particle. A read/write scanner device is used to encode and decode data on the transponder. The stored data includes the sequence of the oligonucleotide immobilized on the transponder. The sequence of sample nucleic acids is determined by detecting annealing to an oligonucleotide bound to a particle, followed by decoding the transponder to determine the sequence of the oligonucleotide.

公开(公告)号：US20130123118A1

公开(公告)日：2013-05-16

申请号：US13643875

申请日：2011-04-29

申请人： Wlodek Mandecki ,  Ji Li ,  Zhuying Wang

发明人： Wlodek Mandecki ,  Ji Li ,  Zhuying Wang

IPC分类号： G01N21/64

CPC分类号： G01N21/6486 ,  G01N33/587 ,  Y10T428/265

摘要： Provided, among other things, is a multiplex assay comprising: conducting a fluorescence-developing assay on microtabs having at least one surface that shows plasmonic enhancement, wherein a plurality of the microtabs have unique probes affixed to their plasmonically enhanced surfaces; and measuring the fluorescence associated with the substrates and identifying the correlated probe by for the microtab. The microtabs can be, for example, MTPs that send a unique identifier, and the correlated probe can be identified by querying the MTPs for their identifier.

摘要翻译： 除其他之外，提供多重测定，其包括：对具有至少一个表现出等离子体激增的表面的微切片进行荧光显影分析，其中多个微切片具有固定于其等离子体增强表面的独特探针; 并测量与底物相关的荧光，并通过microtab鉴定相关探针。 微型计算机可以是例如发送唯一标识符的MTP，并且可以通过查询MTP的标识符来识别相关探测器。

公开(公告)号：US6046003A

公开(公告)日：2000-04-04

申请号：US13114

申请日：1998-01-26

申请人： Wlodek Mandecki

发明人： Wlodek Mandecki

IPC分类号： G01N33/50 ,  B01J19/00 ,  C12N15/09 ,  C12Q1/68 ,  C40B40/06 ,  C40B70/00 ,  G05B23/02 ,  C07H21/04 ,  G08C19/12

CPC分类号： C12Q1/6874 ,  B01J19/0046 ,  C12Q1/6869 ,  B01J2219/005 ,  B01J2219/00567 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00722 ,  C40B40/06 ,  C40B70/00 ,  Y10S435/81 ,  Y10S435/973 ,  Y10T436/143333

摘要： A method is described for determining the sequence of nucleic acids. The method employs small solid phase particles having transponders, with a primary layer of an oligonucleotide of known sequence attached to the outer surface of the particle. A read/write scanner device is used to encode and decode data on the transponder. The stored data includes the sequence of the oligonucleotide immobilized on the transponder. The sequence of sample nucleic acids is determined by detecting annealing to an oligonucleotide bound to a particle, followed by decoding the transponder to determine the sequence of the oligonucleotide.

公开(公告)号：US5641634A

公开(公告)日：1997-06-24

申请号：US565007

申请日：1995-11-30

申请人： Wlodek Mandecki

发明人： Wlodek Mandecki

IPC分类号： C12Q1/68 ,  C40B70/00 ,  G01N15/14 ,  G01N33/543 ,  G01N33/566 ,  G05B23/02 ,  G08C19/12

CPC分类号： C12Q1/6825 ,  C12Q1/6834 ,  G01N33/54313 ,  G01N33/54373 ,  B01J2219/00567 ,  C40B70/00 ,  G01N15/14 ,  Y10S435/81 ,  Y10S435/973 ,  Y10S435/975 ,  Y10S436/805 ,  Y10S436/806 ,  Y10S436/808 ,  Y10S436/824

摘要： Disclosed are materials and methods for detecting biomolecules in samples employing transponders having memory elements associated with particle(s) used as a solid phase in art assay, and information pertinent to the assay is encoded on the transponder memory elements. A dedicated read/write device is used remotely to encode or remotely to read the information encoded on the transponder memory elements. The invention can be used in direct or competitive ELISA-type assays, or in multiplex assays for the simultaneous assay of several analytes.

摘要翻译： 公开了用于在现有技术测定中使用具有与用作固相的颗粒相关的记忆元件的转发器的样品中检测生物分子的材料和方法，并且与应答器存储元件相关的信息被编码。 远程使用专用的读/写设备来编码或远程读取在应答器存储器元件上编码的信息。 本发明可用于直接或竞争性ELISA型测定，或用于多种分析物的同时测定的多重测定中。