公开(公告)号：US20140323595A1

公开(公告)日：2014-10-30

申请号：US14361500

申请日：2012-11-30

Applicant: Agency for Science, Technology and Research ,  Singapore Health Services Pte Ltd.

Inventor： Sai Kiang Lim ,  Kok Hian Tan

IPC: G01N33/68

CPC classification number: G01N33/689 ,  G01N33/5091 ,  G01N33/6872 ,  G01N33/6893 ,  G01N2333/47 ,  G01N2405/10 ,  G01N2800/32 ,  G01N2800/368 ,  G01N2800/52 ,  G01N2800/7028

Abstract: We describe a method of monitoring the state of a cell, tissue, organ or organism. The method comprises establishing, for a sample of microparticles from the cell, tissue, organ or organism, a ratio. The ratio is of a selected polypeptide in microparticles which comprise GM1 gangliosides, preferably which bind to Cholera Toxin B (CTB) (“GM1 ganglioside microparticle polypeptide”) to the selected polypeptide in microparticles which comprise exposed phosphotidylserine, preferably which bind to Annexin V (“Annexin V microparticle polypeptide”). The GM1 ganglioside microparticle polypeptide to Annexin V microparticle polypeptide ratio so established may be indicative of the state of the cell, tissue, organ or organism.

Abstract translation: 我们描述一种监测细胞，组织，器官或生物体状态的方法。 该方法包括为来自细胞，组织，器官或生物体的微粒样品建立一个比例。 该比例是包含GM1神经节苷脂的选择的多肽，其优选结合霍乱毒素B（CTB）（“GM1神经节苷脂微粒多肽”）到包含暴露的磷脂酰丝氨酸的微粒中所选择的多肽，优选结合膜联蛋白V（ “膜联蛋白V微粒多肽”）。 GM1神经节苷脂微粒多肽与膜联蛋白V微粒多肽的比例如此建立可以指示细胞，组织，器官或生物体的状态。

公开(公告)号：US20200054683A1

公开(公告)日：2020-02-20

申请号：US16661657

申请日：2019-10-23

Applicant: Agency for Science, Technology and Research (A*STAR)

Inventor： Sai Kiang Lim

IPC: A61K35/28 ,  A61K39/00

Abstract: We describe the use of exosome such as mesenchymal stem cell exosomes in a method of promoting, restoring or enhancing homeostasis in an individual suffering from graft versus host disease (GVHD) or epidermolysis bullosa (EB). The homeostasis may comprise immune homeostasis such as maintenance of an immune response. The method may comprise administering a therapeutically effective amount of exosome to the individual.

公开(公告)号：US10054599B2

公开(公告)日：2018-08-21

申请号：US14773677

申请日：2014-03-12

Applicant: Agency for Science, Technology and Research (A*STAR) ,  Singapore Health Service PTE LTD

Inventor： Sai Kiang Lim ,  Kok Hian Tan

IPC: G01N33/68 ,  A61K31/166 ,  A61K31/4422 ,  A61K31/4439 ,  A61K31/502 ,  A61K33/06

CPC classification number: G01N33/689 ,  A61K31/166 ,  A61K31/4422 ,  A61K31/4439 ,  A61K31/502 ,  A61K33/06 ,  G01N33/6893 ,  G01N2333/4727 ,  G01N2333/495 ,  G01N2333/5412 ,  G01N2333/58 ,  G01N2333/585 ,  G01N2333/70596 ,  G01N2333/71 ,  G01N2333/8132 ,  G01N2333/8146 ,  G01N2333/9129 ,  G01N2570/00 ,  G01N2800/368 ,  G01N2800/52 ,  G01N2800/60 ,  Y02A50/471

Abstract: We describe a method of detecting pre-eclampsia in a cell, tissue, organ or organism, the method comprising detecting a modulated level of expression, activity or amount of a pre-eclampsia biomarker polypeptide selected from the group consisting of PlGF, FLT1, BNP, ANP, CD9, PAI-1, TGF β, PCT, SI 00b, TIMP1, CD 105 and IL6 in or of a microparticle type (selected from a CTB binding microparticle and an Annexin V binding microparticle) from the cell, tissue, organ or organism, as compared to level of expression, activity or amount of the pre-eclampsia biomarker polypeptide in the same microparticle type in a cell, tissue, organ or organism not sufferin from pre-eclampsia.

公开(公告)号：US09423402B2

公开(公告)日：2016-08-23

申请号：US14361500

申请日：2012-11-30

Applicant: Agency for Science, Technology and Research ,  Singapore Health Services Pte Ltd.

Inventor： Sai Kiang Lim ,  Kok Hian Tan

IPC: C12Q1/00 ,  G01N33/68 ,  G01N33/50

CPC classification number: G01N33/689 ,  G01N33/5091 ,  G01N33/6872 ,  G01N33/6893 ,  G01N2333/47 ,  G01N2405/10 ,  G01N2800/32 ,  G01N2800/368 ,  G01N2800/52 ,  G01N2800/7028

Abstract: We describe a method of monitoring the state of a cell, tissue, organ or organism. The method comprises establishing, for a sample of microparticles from the cell, tissue, organ or organism, a ratio. The ratio is of a selected polypeptide in microparticles which comprise GM1 gangliosides, preferably which bind to Cholera Toxin B (CTB) (“GM1 ganglioside microparticle polypeptide”) to the selected polypeptide in microparticles which comprise exposed phosphotidylserine, preferably which bind to Annexin V (“Annexin V microparticle polypeptide”). The GM1 ganglioside microparticle polypeptide to Annexin V microparticle polypeptide ratio so established may be indicative of the state of the cell, tissue, organ or organism.

Abstract translation: 我们描述一种监测细胞，组织，器官或生物体状态的方法。 该方法包括为来自细胞，组织，器官或生物体的微粒样品建立一个比例。 该比例是包含GM1神经节苷脂的选择的多肽，其优选结合霍乱毒素B（CTB）（“GM1神经节苷脂微粒多肽”）到包含暴露的磷脂酰丝氨酸的微粒中所选择的多肽，优选结合膜联蛋白V（ “膜联蛋白V微粒多肽”）。 GM1神经节苷脂微粒多肽与膜联蛋白V微粒多肽的比例如此建立可以指示细胞，组织，器官或生物体的状态。