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34 条记录 (耗时 0.019 秒)

    Alkaline pullulanase Y having .alpha.-amylase activity
    11.
    发明授权
    Alkaline pullulanase Y having .alpha.-amylase activity 失效
    ALKALINE PULLULANASE Y HAVING（ALPHA） - 酰胺酶活性

    公开(公告)号：US5147796A

    公开(公告)日：1992-09-15

    申请号：US825314

    申请日：1992-01-27

    申请人： Katsutoshi Ara ,  Katsuhisa Saeki ,  Kazuaki Igarashi ,  Susumu Ito

    发明人： Katsutoshi Ara ,  Katsuhisa Saeki ,  Kazuaki Igarashi ,  Susumu Ito

    IPC分类号： C12N9/44

    CPC分类号： C12N9/2417 ,  C12N9/2457 ,  C12Y302/01041 ,  Y10S435/832

    摘要： An isolated alkaline pullulanase Y having .alpha.-amylase activity; a microorganism producing the alkaline pullulanase Y; and a process for producing the alkaline pullulanase Y are disclosed. The alkaline pullulanase Y having .alpha.-amylase activity has its optimum pH at higher alkaline range than conventional alkaline pullulanases and exhibits excellent stability in a wide pH range. Further, the alkaline pullulanase Y has strong resistance to almost all detergent components such as surfactants, chelating agents, proteases for detergents, and the like. Thus the alkaline pullulanase Y can advantageously be used as a detergent component.

    摘要翻译： 具有α-淀粉酶活性的分离的碱性支链淀粉酶Y; 产生碱性支链淀粉酶Y的微生物; 并公开了制备碱性支链淀粉酶Y的方法。 具有α-淀粉酶活性的碱性支链淀粉酶Y具有比常规碱性支链淀粉酶更高的碱性范围的最佳pH，并且在宽pH范围内表现出优异的稳定性。 此外，碱性支链淀粉酶Y对几乎所有的洗涤剂组分如表面活性剂，螯合剂，用于洗涤剂的蛋白酶等都具有很强的抗性。 因此，碱性支链淀粉酶Y可以有利地用作洗涤剂组分。

    Alkaline pullulanase from Bacillus sp. FERM P-10887
    12.
    发明授权
    Alkaline pullulanase from Bacillus sp. FERM P-10887 失效
    来自BACILLUS SP的ALKALINE PULLULANASE FERM P-10887

    公开(公告)号：US5147795A

    公开(公告)日：1992-09-15

    申请号：US575434

    申请日：1990-08-30

    申请人： Katsutoshi Ara ,  Kazuaki Igarashi ,  Katsuhisa Saeki ,  Susumu Ito

    发明人： Katsutoshi Ara ,  Kazuaki Igarashi ,  Katsuhisa Saeki ,  Susumu Ito

    IPC分类号： C12N9/44

    CPC分类号： C12Y302/01041 ,  C12N9/2457 ,  Y10S435/832

    摘要： A novel alkaline pullulanase; a microorganism producing the alkaline pullulanase; and a process for producing the alkaline pullulanase are disclosed. The alkaline pullulanase of the present invention has a higher optimum pH range (pH 9.5-11) than conventional alkaline pullulanases and shows excellent stability in a wider pH range. It has also an optimum temperature of higher than 50.degree. C. and is thermally stable up to 40.degree. C. The alkaline pullulanase has also strong reistance to almost all detergent components such as surfactants, chelating agents, proteases for detergents, and the like. The alkaline pullulanase can advantageously be used as a detergent component. Also, it can be utilized for producing many kinds of oligosaccharides and also together with .alpha.-amylase for producing various monosaccharides.

    Recombinant microorganism that expresses a secY gene with deletion of sporulation-associated genes and method of producing thereof
    14.
    发明授权
    Recombinant microorganism that expresses a secY gene with deletion of sporulation-associated genes and method of producing thereof 有权
    表达具有孢子形成相关基因缺失的secY基因的重组微生物及其制备方法

    公开(公告)号：US08389264B2

    公开(公告)日：2013-03-05

    申请号：US12530135

    申请日：2008-04-08

    申请人： Shenghao Liu ,  Keiji Endo ,  Katsutoshi Ara

    发明人： Shenghao Liu ,  Keiji Endo ,  Katsutoshi Ara

    IPC分类号： C12N1/21

    CPC分类号： C12P21/02

    摘要： A recombinant microorganism having improved productivity for a protein or a polypeptide, and a method for producing a protein or a polypeptide using the recombinant microorganism, are provided.A recombinant microorganism obtained by transfecting a gene for encoding a desired protein or polypeptide into a microorganism strain which is obtained by genetically constructing to overexpress secY gene of Bacillus subtilis or a gene corresponding to the secY gene, and deleting or inactivating one or more genes selected from sporulation-associated genes and genes corresponding to the sporulation-associated genes from the genome.

    摘要翻译： 提供了具有提高的蛋白质或多肽的生产力的重组微生物，以及使用该重组微生物生产蛋白质或多肽的方法。 将通过将所需蛋白质或多肽编码的基因转染到通过遗传构建以过表达枯草芽孢杆菌的secY基因或相应于所述secY基因的基因而获得的微生物菌株获得的重组微生物，以及删除或灭活所选择的一种或多种基因 来自与来自基因组的孢子形成相关基因相对应的孢子形成相关基因和基因。

    Modified Microorganism
    15.
    发明申请
    Modified Microorganism 有权
    改良微生物

    公开(公告)号：US20100093032A1

    公开(公告)日：2010-04-15

    申请号：US12530710

    申请日：2008-04-01

    申请人： Katsutoshi Ara ,  Hiroshi Kakeshita ,  Kouji Nakamura

    发明人： Katsutoshi Ara ,  Hiroshi Kakeshita ,  Kouji Nakamura

    IPC分类号： C12P21/06 ,  C12N1/00

    CPC分类号： C07K14/32 ,  C12P21/02

    摘要： To provide a microorganism with enhanced secretory production of a protein or polypeptide and a method of producing the protein or polypeptide using the microorganism. A modified microorganism that has been genetically modified to delete 60 to 80 carboxyl-terminal amino acids of SecA.

    摘要翻译： 提供具有增强的蛋白质或多肽的分泌生产的微生物和使用该微生物产生蛋白质或多肽的方法。 已被遗传修饰以修复SecA的60至80个羧基末端氨基酸的经修饰的微生物。

    DNA fragment containing gene for alkaline pullulanase
    16.
    发明授权
    DNA fragment containing gene for alkaline pullulanase 失效
    含有支链淀粉酶基因的DNA片段

    公开(公告)号：US06509183B1

    公开(公告)日：2003-01-21

    申请号：US08307589

    申请日：1994-09-28

    申请人： Katsutoshi Ara ,  Kazuaki Igarashi ,  Katsuhisa Saeki ,  Shuji Kawai ,  Susumu Ito

    发明人： Katsutoshi Ara ,  Kazuaki Igarashi ,  Katsuhisa Saeki ,  Shuji Kawai ,  Susumu Ito

    IPC分类号： C12N944

    CPC分类号： C12N9/2457 ,  C12N9/2451 ,  C12Y302/01041

    摘要： A DNA fragment coding for alkaline pullulanase, which contains about 6.3 Kb base pairs and has a restriction map shown in FIG. 2, a recombinant vector containing the DNA fragment, and a microorganism carrying the vector. Also disclosed is a method of preparing alkaline pullulanase which comprises culturing a transformant microorganism which has been transformed with a recombinant vector containing a DNA fragment coding for alkaline pullulanase, having a restriction enzyme map shown in FIG. 2 and having about 6.3 Kb base pairs. According to the method of the invention, alkaline pullulanase which is useful as a component of detergents can be mass-produced in a low cost.

    摘要翻译： 编码碱性支链淀粉酶的DNA片段，其含有约6.3Kb碱基对并且具有图1所示的限制性图谱。 2，含有DNA片段的重组载体和携带载体的微生物。还公开了制备碱性支链淀粉酶的方法，其包括培养已经用含有编码碱性支链淀粉酶的DNA片段的重组载体转化的转化体微生物，其具有 图1所示的限制酶图谱。 并且具有约6.3Kb碱基对。根据本发明的方法，可以以低成本批量生产用作洗涤剂组分的碱性支链淀粉酶。

    Detergent composition containing an alkaline pullulanase from bacillus ferm BP-3048
    18.
    发明授权
    Detergent composition containing an alkaline pullulanase from bacillus ferm BP-3048 失效
    含有来自芽孢杆菌BP-3048的碱性支链淀粉酶的洗涤剂组合物

    公开(公告)号：US5316691A

    公开(公告)日：1994-05-31

    申请号：US960262

    申请日：1992-10-13

    申请人： Taeko Sone ,  Masaki Tosaka ,  Katsuhisa Saeki ,  Katsutoshi Ara ,  Katsuhiko Deguchi ,  Kazuaki Igarashi

    发明人： Taeko Sone ,  Masaki Tosaka ,  Katsuhisa Saeki ,  Katsutoshi Ara ,  Katsuhiko Deguchi ,  Kazuaki Igarashi

    IPC分类号： C11D3/386 ,  C12N9/44 ,  C11D1/00 ,  C11D3/00 ,  C11D17/00

    CPC分类号： C11D3/38636 ,  C12N9/2451 ,  C12N9/2457 ,  C12Y302/01041 ,  Y10S435/832

    摘要： A detergent composition containing an alkaline pullulanase, a surfactant, alkaline agents and/or inorganic electrolytes, divalent metal ion scavengers and bleaching agents is disclosed. The alkaline pullulanase has an optimum pH range of 8.5-10.0 on pullulan, an optimum temperature of about 50.degree. C. and is not deactivated by surfactants. Further, the pullulanase has a strong resistance to almost all detergent components such as chelating agents, proteases, etc. The pullulanase is isolated from Bacillus sp. KSM-AP 1378 deposited as FERM BP-3048. The composition specifically contains 0.1-10 wt. % alkaline pullulanase B, 0.5-60 wt. % surfactant, 0-90 wt. % alkaline agents and/or inorganic electrolytes, 0-50 wt. % divalent metal ion scavengers, and 0-85 wt. % bleaching agents.

    摘要翻译： 公开了含有碱性支链淀粉酶，表面活性剂，碱剂和/或无机电解质，二价金属离子清除剂和漂白剂的洗涤剂组合物。 碱性支链淀粉酶在支链淀粉上的最适pH范围为8.5-10.0，最适温度为约50℃，不被表面活性剂失活。 此外，支链淀粉酶对几乎所有的洗涤剂组分如螯合剂，蛋白酶等都具有很强的抗性。支链淀粉酶从芽孢杆菌中分离出来。 KSM-AP 1378保存为FERM BP-3048。 该组合物具体含有0.1-10重量％ ％碱性支链淀粉酶B，0.5-60wt。 ％表面活性剂，0-90重量％ ％碱性试剂和/或无机电解质，0-50wt。 ％的二价金属离子清除剂，和0-85wt。 ％漂白剂。

    Recombinant microorganism expressing a secY gene and method of use thereof
    20.
    发明授权
    Recombinant microorganism expressing a secY gene and method of use thereof 有权
    表达SecY基因的重组微生物及其使用方法

    公开(公告)号：US08460893B2

    公开(公告)日：2013-06-11

    申请号：US12279271

    申请日：2007-02-15

    申请人： Keiji Endo ,  Shenghao Liu ,  Katsutoshi Ara

    发明人： Keiji Endo ,  Shenghao Liu ,  Katsutoshi Ara

    IPC分类号： C12Q1/34 ,  C12N1/21 ,  C12P21/00 ,  C12N15/00 ,  C12N9/42

    CPC分类号： C12N9/2437

    摘要： The object of the invention is to provide a microorganism which enhances productivity of cellulase and is useful for industrial production of cellulase, and to provide a method for producing cellulase by use of the microorganism. The present invention provides a recombinant microorganism produced by transferring a gene encoding cellulase to a parental microorganism which has been genetically modified so as to overexpress a Bacillus subtilis secY gene or a gene corresponding thereto.

    摘要翻译： 本发明的目的是提供一种提高纤维素酶生产率并可用于纤维素酶的工业生产的微生物，并提供使用微生物生产纤维素酶的方法。 本发明提供了一种通过将编码纤维素酶的基因转移到已被遗传修饰以使其过表达枯草芽孢杆菌SecY基因或相应基因的亲本微生物而产生的重组微生物。