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    Membrane-translocating peptides
    11.
    发明授权
    Membrane-translocating peptides 有权
    膜易位肽

    公开(公告)号：US08557744B2

    公开(公告)日：2013-10-15

    申请号：US11996496

    申请日：2006-07-24

    申请人： David Coomber ,  Kevin Fitzgerald ,  Duncan McGregor ,  Chris Ullman ,  Tomas Leanderson

    发明人： David Coomber ,  Kevin Fitzgerald ,  Duncan McGregor ,  Chris Ullman ,  Tomas Leanderson

    IPC分类号： C40B30/04 ,  C12Q1/70

    CPC分类号： C12N15/1075

    摘要： A method is selects membrane-translocating peptides (MTPs) from a peptide display library that are capable of crossing or penetrating a lipid membrane. A plurality of nucleic acid constructs that encode displayed peptides are expressed, resulting in the formation of a plurality of nucleic acid-peptide complexes, each complex comprising at least one displayed peptide associated with the corresponding nucleic acid construct encoding the displayed peptide; the complexes are exposed to a population of membrane-encapsulated compartments, allowing a translocating reaction to occur; complexes that remain unassociated with the membrane are removed; optionally complexes that are associated with the membrane are removed; and internalized nucleic acid-peptide complexes are recovered. The membrane-encapsulated compartments may be artificial vesicles such as liposomes, or populations of one or more cell types.

    摘要翻译： 一种方法是从能够穿透或穿透脂质膜的肽展示文库中选择膜 - 位移肽（MTP）。 表达编码显示的肽的多个核酸构建体，导致形成多个核酸 - 肽复合物，每个复合物包含与编码所显示肽的相应核酸构建体相关联的至少一个显示的肽; 复合物暴露于膜包封的隔室群，允许发生易位反应; 与膜保持不相关的复合物被去除; 去除与膜相关的任选复合物; 并回收内化的核酸 - 肽复合物。 膜包封的隔室可以是人造囊泡，例如脂质体或一种或多种细胞类型的群体。

    Compostions and methods for enhancing oligonucleotide delivery across and into epithelial tissues
    14.
    发明申请
    Compostions and methods for enhancing oligonucleotide delivery across and into epithelial tissues 审中-公开
    用于增强穿过和进入上皮组织的寡核苷酸递送的组合物和方法

    公开(公告)号：US20100222417A1

    公开(公告)日：2010-09-02

    申请号：US12591629

    申请日：2009-11-25

    申请人： Tracy Zimmermann ,  Kevin Fitzgerald ,  Muthiah Manoharan

    发明人： Tracy Zimmermann ,  Kevin Fitzgerald ,  Muthiah Manoharan

    IPC分类号： A61K31/7088 ,  A61K31/56

    CPC分类号： A61K31/56 ,  A61K31/7088

    摘要： The present invention relates to the delivery of oligonucleotide across and into epithelial tissues by conjugating the oligonucleotide with a lipophile and/or coadministering with a penetration enhancer. In particular, the present invention provides a composition comprising a conjugated siRNA, which are advantageous for the in vivo delivery of nucleic acids across and into epithelial tissue. Additionally, the present invention provides methods of improving delivery of oligonucleotides across the epithelial tissues with the aid of a mechanical enhancer.

    摘要翻译： 本发明涉及通过将寡核苷酸与亲脂体共轭和/或与渗透增强剂共同引发而将寡核苷酸递送到上皮组织中并进入上皮组织。 特别地，本发明提供了包含缀合siRNA的组合物，其有利于体内递送穿过和进入上皮组织的核酸。 另外，本发明提供了借助于机械增强剂来改善跨越上皮组织的寡核苷酸递送的方法。