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    Bacillus thuringiensis cry gene and protein
    12.
    发明授权
    Bacillus thuringiensis cry gene and protein 失效
    苏云金芽孢杆菌cry基因和蛋白质

    公开(公告)号：US07476781B2

    公开(公告)日：2009-01-13

    申请号：US11692973

    申请日：2007-03-29

    申请人： Andre R. Abad ,  Ronald D. Flannagan ,  Billy F. McCutchen ,  Cao Guo Yu

    发明人： Andre R. Abad ,  Ronald D. Flannagan ,  Billy F. McCutchen ,  Cao Guo Yu

    IPC分类号： A01H5/00 ,  A01H5/10 ,  C12N15/82

    CPC分类号： C07K14/325 ,  C12N15/8286 ,  Y02A40/162

    摘要： Compositions and methods for protecting a plant from an insect pest are provided. In particular, novel polynucleotides and the pesticidal polypeptides they encode are provided. Methods of using the novel polynucleotides and pesticidal polypeptides of the invention to protect a plant from an insect pest are further provided. Particular embodiments of the invention provide pesticidal compositions and formulations, DNA constructs, and transformed plants, plant cells, and seeds.

    摘要翻译： 提供了保护植物免受害虫害虫的组合物和方法。 特别地，提供了新型多核苷酸及其编码的杀虫多肽。 还提供了使用本发明的新型多核苷酸和杀虫多肽保护植物免受害虫的方法。 本发明的具体实施方案提供杀虫组合物和制剂，DNA构建体和转化的植物，植物细胞和种子。

    PLANT ACTIVATION OF INSECT TOXIN
    15.
    发明申请
    PLANT ACTIVATION OF INSECT TOXIN 有权
    植物激活的毒素

    公开(公告)号：US20090317891A1

    公开(公告)日：2009-12-24

    申请号：US12512425

    申请日：2009-07-30

    申请人： Andre Abad ,  Ronald D. Flannagan ,  Rafael Hermann ,  Albert L. Lu ,  Billy F. McCutchen ,  James K. Presnail ,  Janet A. Rice ,  James F. Wong ,  Cao-Guo Yu

    发明人： Andre Abad ,  Ronald D. Flannagan ,  Rafael Hermann ,  Albert L. Lu ,  Billy F. McCutchen ,  James K. Presnail ,  Janet A. Rice ,  James F. Wong ,  Cao-Guo Yu

    IPC分类号： C12N1/00 ,  C07H21/00 ,  C12N15/74

    CPC分类号： C12N15/8286 ,  C12N9/641 ,  Y02A40/162

    摘要： Compositions and methods for protecting a plant from an insect pest are provided. In particular, nucleic acid sequences encoding insect protoxins modified to comprise at least one proteolytic activation site that is sensitive to a plant protease or an insect gut protease are provided. Cleavage of the modified protoxin at the proteolytic activation site by a protease produces an active insect toxin. Methods of using the modified insect protoxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds.

    摘要翻译： 提供了保护植物免受害虫害虫的组合物和方法。 特别地，提供了编码昆虫原毒素的核酸序列，其被修饰为包含对植物蛋白酶或昆虫肠道蛋白酶敏感的至少一个蛋白水解活化位点。 通过蛋白酶在蛋白水解活化位点切割修饰的原毒素会产生活性昆虫毒素。 提供了使用修饰的昆虫原毒素核酸序列及其编码的多肽保护植物免受害虫的方法。 本发明的具体实施方案还提供了改良的昆虫原毒素组合物和制剂，表达盒，以及转化的植物，植物细胞和种子。

    Plant Activation of Insect Toxin
    16.
    发明申请
    Plant Activation of Insect Toxin 有权
    植物激活昆虫毒素

    公开(公告)号：US20080200415A1

    公开(公告)日：2008-08-21

    申请号：US11940002

    申请日：2007-11-14

    申请人： Andre Abad ,  Ronald D. Flannagan ,  Rafael Herrmann ,  Albert L. Lu ,  Billy F. McCutchen ,  James K. Presnail ,  Janet A. Rice ,  James F. Wong ,  Cao-Guo Yu

    发明人： Andre Abad ,  Ronald D. Flannagan ,  Rafael Herrmann ,  Albert L. Lu ,  Billy F. McCutchen ,  James K. Presnail ,  Janet A. Rice ,  James F. Wong ,  Cao-Guo Yu

    IPC分类号： A61K31/7088 ,  C12N15/32 ,  A01P7/04 ,  A01H5/00

    CPC分类号： C12N15/8286 ,  C12N9/641 ,  Y02A40/162

    摘要： Compositions and methods for protecting a plant from an insect pest are provided. In particular, nucleic acid sequences encoding insect protoxins modified to comprise at least one proteolytic activation site that is sensitive to a plant protease or an insect gut protease are provided. Cleavage of the modified protoxin at the proteolytic activation site by a protease produces an active insect toxin. Methods of using the modified insect protoxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds.

    摘要翻译： 提供了保护植物免受害虫害虫的组合物和方法。 特别地，提供了编码昆虫原毒素的核酸序列，其被修饰为包含对植物蛋白酶或昆虫肠道蛋白酶敏感的至少一个蛋白水解活化位点。 通过蛋白酶在蛋白水解活化位点切割修饰的原毒素会产生活性昆虫毒素。 提供了使用修饰的昆虫原毒素核酸序列及其编码的多肽保护植物免受害虫的方法。 本发明的具体实施方案还提供了改良的昆虫原毒素组合物和制剂，表达盒，以及转化的植物，植物细胞和种子。

    PLANT ACTIVATION OF INSECT TOXIN
    17.
    发明申请
    PLANT ACTIVATION OF INSECT TOXIN 失效
    植物激活的毒素

    公开(公告)号：US20080124799A1

    公开(公告)日：2008-05-29

    申请号：US11949912

    申请日：2007-12-04

    申请人： Andre Abad ,  Ronald D. Flannagan ,  Rafael Herrmann ,  Albert L. Lu ,  Billy F. McCutchen ,  James K. Presnail ,  Janet A. Rice ,  James F. Wong ,  Cao-Guo Yu

    发明人： Andre Abad ,  Ronald D. Flannagan ,  Rafael Herrmann ,  Albert L. Lu ,  Billy F. McCutchen ,  James K. Presnail ,  Janet A. Rice ,  James F. Wong ,  Cao-Guo Yu

    IPC分类号： C12N5/04 ,  C07H21/04 ,  C12N15/00

    CPC分类号： C12N15/8286 ,  C12N9/641 ,  Y02A40/162

    摘要： Compositions and methods for protecting a plant from an insect pest are provided. In particular, nucleic acid sequences encoding insect protoxins modified to comprise at least one proteolytic activation site that is sensitive to a plant protease or an insect gut protease are provided. Cleavage of the modified protoxin at the proteolytic activation site by a protease produces an active insect toxin. Methods of using the modified insect protoxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds.

    摘要翻译： 提供了保护植物免受害虫害虫的组合物和方法。 特别地，提供了编码昆虫原毒素的核酸序列，其被修饰为包含对植物蛋白酶或昆虫肠道蛋白酶敏感的至少一个蛋白水解活化位点。 通过蛋白酶在蛋白水解活化位点切割修饰的原毒素会产生活性昆虫毒素。 提供了使用修饰的昆虫原毒素核酸序列及其编码的多肽保护植物免受害虫的方法。 本发明的具体实施方案还提供了改良的昆虫原毒素组合物和制剂，表达盒，以及转化的植物，植物细胞和种子。

    Genes encoding novel Bacillus thuringiensis proteins with pesticidal activity against Coleopterans
    20.
    发明授权
    Genes encoding novel Bacillus thuringiensis proteins with pesticidal activity against Coleopterans 有权
    编码具有杀虫活性的新型苏云金芽孢杆菌蛋白的基因

    公开(公告)号：US07696412B2

    公开(公告)日：2010-04-13

    申请号：US10414637

    申请日：2003-04-16

    申请人： Andre R. Abad ,  Nicholas B. Duck ,  Xiang Feng ,  Ronald D. Flannagan ,  Theodore W. Kahn ,  Lynne E. Sims

    发明人： Andre R. Abad ,  Nicholas B. Duck ,  Xiang Feng ,  Ronald D. Flannagan ,  Theodore W. Kahn ,  Lynne E. Sims

    IPC分类号： A01H5/00 ,  A01H5/10 ,  C12N15/82 ,  C12N15/32

    CPC分类号： C07K14/325 ,  C12N15/8286 ,  Y02A40/162

    摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding δ-endotoxins having pesticidal activity against pests of the order Coleoptera. The invention further provides mutagenized nucleic acids that have been modified to encode endotoxins having improved pesticidal activity and/or altered pest specificity. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, expression cassettes comprising such nucleic acids, and transformed plants and seeds comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.

    摘要翻译： 本发明提供从编号δ-内毒素的苏云金芽孢杆菌菌株获得的核酸及其变体和片段，其具有针对鞘翅目害虫的杀虫活性。 本发明还提供已被修饰以编码具有改善的杀虫活性和/或改变的有害生物特异性的内毒素的诱变的核酸。 本发明的具体实施方案提供编码杀虫蛋白的分离的核酸，包含此类核酸的表达盒，以及包含本发明核酸的转化植物和种子。 这些组合物可用于防治害虫，特别是植物害虫的方法。