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公开(公告)号：US07105332B2
公开(公告)日：2006-09-12
申请号：US11108389
申请日：2005-04-18
申请人： André Abad , Ronald D. Flannagan , Rafael Herrmann , Theodore W. Kahn , Albert L. Lu , Billy F. McCutchen , James K. Presnail , James F. H. Wong , Cao-Guo Yu
发明人： André Abad , Ronald D. Flannagan , Rafael Herrmann , Theodore W. Kahn , Albert L. Lu , Billy F. McCutchen , James K. Presnail , James F. H. Wong , Cao-Guo Yu
CPC分类号： C07K14/325 , C12N15/8286 , Y02A40/162
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding δ-endotoxins having pesticidal activity against insect pests. The invention further provides mutagenized nucleic acids that have been modified to encode pesticidal polypeptides such as endotoxins having improved pesticidal activity and/or altered pest specificity. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins that may be optimized as well as pesticidal compositions, expression cassettes, and transformed microorganisms and plants comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.
摘要翻译： 本发明提供从编码具有杀虫活性的昆虫害虫的δ-内毒素的苏云金芽孢杆菌菌株获得的核酸及其变体和片段。 本发明进一步提供已被修饰以编码杀虫多肽的诱变核酸，例如具有改善的杀虫活性和/或改变的有害生物特异性的内毒素。 本发明的具体实施方案提供编码可能被优化的杀虫蛋白质的分离的核酸，以及包含本发明的核酸的农药组合物，表达盒和转化的微生物和植物。 这些组合物可用于防治害虫，特别是植物害虫的方法。
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公开(公告)号：US08071736B2
公开(公告)日：2011-12-06
申请号：US12512425
申请日：2009-07-30
申请人： Andre R. Abad , Ronald D. Flannagan , Rafael Herrmann , Albert L. Lu , Billy F. McCutchen , James K. Presnail , Janet A. Rice , James F. Wong , Cao-Guo Yu
发明人： Andre R. Abad , Ronald D. Flannagan , Rafael Herrmann , Albert L. Lu , Billy F. McCutchen , James K. Presnail , Janet A. Rice , James F. Wong , Cao-Guo Yu
CPC分类号： C12N15/8286 , C12N9/641 , Y02A40/162
摘要： Compositions and methods for protecting a plant from an insect pest are provided. In particular, nucleic acid sequences encoding insect protoxins modified to comprise at least one proteolytic activation site that is sensitive to a plant protease or an insect gut protease are provided. Cleavage of the modified protoxin at the proteolytic activation site by a protease produces an active insect toxin. Methods of using the modified insect protoxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds.
摘要翻译： 提供了保护植物免受害虫害虫的组合物和方法。 特别地，提供了编码昆虫原毒素的核酸序列，其被修饰为包含对植物蛋白酶或昆虫肠道蛋白酶敏感的至少一个蛋白水解活化位点。 通过蛋白酶在蛋白水解活化位点切割修饰的原毒素会产生活性昆虫毒素。 提供了使用修饰的昆虫原毒素核酸序列及其编码的多肽保护植物免受害虫的方法。 本发明的具体实施方案还提供了改良的昆虫原毒素组合物和制剂，表达盒，以及转化的植物，植物细胞和种子。
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公开(公告)号：US07629449B2
公开(公告)日：2009-12-08
申请号：US11949912
申请日：2007-12-04
申请人： Andre R. Abad , Ronald D. Flannagan , Rafael Herrmann , Albert L. Lu , Billy F. McCutchen , James K. Presnail , Janet A. Rice , James F. Wong , Cao-Guo Yu
发明人： Andre R. Abad , Ronald D. Flannagan , Rafael Herrmann , Albert L. Lu , Billy F. McCutchen , James K. Presnail , Janet A. Rice , James F. Wong , Cao-Guo Yu
CPC分类号： C12N15/8286 , C12N9/641 , Y02A40/162
摘要： In particular aspects of the invention, nucleic acid molecules encoding novel proteases identified in the midgut of Diabrotica virgfera virgfera (i.e., western corn rootworm) are provided. The nucleotide sequences encode novel cysteine proteases that belong to the cathepsin L-like subfamily of proteases. Variants and fragments of the nucleotide sequences that encode polypeptide sequences that possess proteolytic activity are further disclosed. Methods of using the nucleic acid sequences that encode novel proteases to protect a plant from an insect pest are provided. Particular embodiments also provide vectors, expression cassettes and transformed host cells comprising a protease nucleic acid of the invention.
摘要翻译： 在本发明的特定方面，提供了编码在维生素Diabrotica virgfera（即西方玉米根虫）的中肠中鉴定的新型蛋白酶的核酸分子。 核苷酸序列编码属于蛋白酶组织蛋白酶L样亚类的新型半胱氨酸蛋白酶。 还公开了编码具有蛋白水解活性的多肽序列的核苷酸序列的变体和片段。 提供了使用编码新型蛋白酶的核酸序列来保护植物免受害虫的方法。 具体实施方案还提供载体，表达盒和包含本发明的蛋白酶核酸的转化宿主细胞。
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公开(公告)号：US20080200415A1
公开(公告)日：2008-08-21
申请号：US11940002
申请日：2007-11-14
申请人： Andre Abad , Ronald D. Flannagan , Rafael Herrmann , Albert L. Lu , Billy F. McCutchen , James K. Presnail , Janet A. Rice , James F. Wong , Cao-Guo Yu
发明人： Andre Abad , Ronald D. Flannagan , Rafael Herrmann , Albert L. Lu , Billy F. McCutchen , James K. Presnail , Janet A. Rice , James F. Wong , Cao-Guo Yu
IPC分类号： A61K31/7088 , C12N15/32 , A01P7/04 , A01H5/00
CPC分类号： C12N15/8286 , C12N9/641 , Y02A40/162
摘要： Compositions and methods for protecting a plant from an insect pest are provided. In particular, nucleic acid sequences encoding insect protoxins modified to comprise at least one proteolytic activation site that is sensitive to a plant protease or an insect gut protease are provided. Cleavage of the modified protoxin at the proteolytic activation site by a protease produces an active insect toxin. Methods of using the modified insect protoxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds.
摘要翻译： 提供了保护植物免受害虫害虫的组合物和方法。 特别地，提供了编码昆虫原毒素的核酸序列，其被修饰为包含对植物蛋白酶或昆虫肠道蛋白酶敏感的至少一个蛋白水解活化位点。 通过蛋白酶在蛋白水解活化位点切割修饰的原毒素会产生活性昆虫毒素。 提供了使用修饰的昆虫原毒素核酸序列及其编码的多肽保护植物免受害虫的方法。 本发明的具体实施方案还提供了改良的昆虫原毒素组合物和制剂，表达盒，以及转化的植物，植物细胞和种子。
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公开(公告)号：US20080124799A1
公开(公告)日：2008-05-29
申请号：US11949912
申请日：2007-12-04
申请人： Andre Abad , Ronald D. Flannagan , Rafael Herrmann , Albert L. Lu , Billy F. McCutchen , James K. Presnail , Janet A. Rice , James F. Wong , Cao-Guo Yu
发明人： Andre Abad , Ronald D. Flannagan , Rafael Herrmann , Albert L. Lu , Billy F. McCutchen , James K. Presnail , Janet A. Rice , James F. Wong , Cao-Guo Yu
CPC分类号： C12N15/8286 , C12N9/641 , Y02A40/162
摘要： Compositions and methods for protecting a plant from an insect pest are provided. In particular, nucleic acid sequences encoding insect protoxins modified to comprise at least one proteolytic activation site that is sensitive to a plant protease or an insect gut protease are provided. Cleavage of the modified protoxin at the proteolytic activation site by a protease produces an active insect toxin. Methods of using the modified insect protoxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds.
摘要翻译： 提供了保护植物免受害虫害虫的组合物和方法。 特别地，提供了编码昆虫原毒素的核酸序列，其被修饰为包含对植物蛋白酶或昆虫肠道蛋白酶敏感的至少一个蛋白水解活化位点。 通过蛋白酶在蛋白水解活化位点切割修饰的原毒素会产生活性昆虫毒素。 提供了使用修饰的昆虫原毒素核酸序列及其编码的多肽保护植物免受害虫的方法。 本发明的具体实施方案还提供了改良的昆虫原毒素组合物和制剂，表达盒，以及转化的植物，植物细胞和种子。
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6.发明授权Plant activation of Cry8Bb1 by insertion of a plant protease-sensitive site 有权通过插入植物蛋白酶敏感位点植物激活Cry8Bb1公开(公告)号：US07339092B2
公开(公告)日：2008-03-04
申请号：US11021115
申请日：2004-12-22
申请人： Andre Abad , Ronald D. Flannagan , Rafael Herrmann , Albert L. Lu , Billy F. McCutchen , James K. Presnail , Janet A. Rice , James F. Wong , Cao-Guo Yu
发明人： Andre Abad , Ronald D. Flannagan , Rafael Herrmann , Albert L. Lu , Billy F. McCutchen , James K. Presnail , Janet A. Rice , James F. Wong , Cao-Guo Yu
CPC分类号： C12N15/8286 , C12N9/641 , Y02A40/162
摘要： Compositions and methods for protecting a plant from an insect pest are provided. In particular, nucleic acid sequences encoding insect protoxins modified to comprise at least one proteolytic activation site that is sensitive to a plant protease or an insect gut protease are provided. Cleavage of the modified protoxin at the proteolytic activation site by a protease produces an active insect toxin. Methods of using the modified insect protoxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds.
摘要翻译： 提供了保护植物免受害虫害虫的组合物和方法。 特别地，提供了编码昆虫原毒素的核酸序列，其被修饰为包含对植物蛋白酶或昆虫肠道蛋白酶敏感的至少一个蛋白水解活化位点。 通过蛋白酶在蛋白水解活化位点切割修饰的原毒素会产生活性昆虫毒素。 提供了使用修饰的昆虫原毒素核酸序列及其编码的多肽保护植物免受害虫的方法。 本发明的具体实施方案还提供了改良的昆虫原毒素组合物和制剂，表达盒，以及转化的植物，植物细胞和种子。
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公开(公告)号：US07910807B2
公开(公告)日：2011-03-22
申请号：US11940002
申请日：2007-11-14
申请人： Andre R. Abad , Ronald D. Flannagan , Rafael Herrmann , Albert L. Lu , Billy F. McCutchen , James K. Presnail , Janet A. Rice , James F. Wong , Cao-Guo Yu
发明人： Andre R. Abad , Ronald D. Flannagan , Rafael Herrmann , Albert L. Lu , Billy F. McCutchen , James K. Presnail , Janet A. Rice , James F. Wong , Cao-Guo Yu
CPC分类号： C12N15/8286 , C12N9/641 , Y02A40/162
摘要： Compositions and methods for protecting a plant from an insect pest are provided. In particular, nucleic acid sequences encoding insect protoxins modified to comprise at least one proteolytic activation site that is sensitive to a plant protease or an insect gut protease are provided. Cleavage of the modified protoxin at the proteolytic activation site by a protease produces an active insect toxin. Methods of using the modified insect protoxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds.
摘要翻译： 提供了保护植物免受害虫害虫的组合物和方法。 特别地，提供了编码昆虫原毒素的核酸序列，其被修饰为包含对植物蛋白酶或昆虫肠道蛋白酶敏感的至少一个蛋白水解活化位点。 通过蛋白酶在蛋白水解活化位点切割修饰的原毒素会产生活性昆虫毒素。 提供了使用修饰的昆虫原毒素核酸序列及其编码的多肽保护植物免受害虫的方法。 本发明的具体实施方案还提供了改良的昆虫原毒素组合物和制剂，表达盒，以及转化的植物，植物细胞和种子。
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公开(公告)号：US07556936B2
公开(公告)日：2009-07-07
申请号：US11950509
申请日：2007-12-05
申请人： Andre R. Abad , Ronald D. Flannagan , Rafael Herrmann , Albert L. Lu , Billy F. McCutchen , James K. Presnail , Janet A. Rice , James F. Wong , Cao-Guo Yu
发明人： Andre R. Abad , Ronald D. Flannagan , Rafael Herrmann , Albert L. Lu , Billy F. McCutchen , James K. Presnail , Janet A. Rice , James F. Wong , Cao-Guo Yu
CPC分类号： C12N15/8286 , C12N9/641 , Y02A40/162
摘要： Compositions and methods for protecting a plant from an insect pest are provided. In particular, nucleic acid sequences encoding insect protoxins modified to comprise at least one proteolytic activation site that is sensitive to a plant protease or an insect gut protease are provided. Cleavage of the modified protoxin at the proteolytic activation site by a protease produces an active insect toxin. Methods of using the modified insect protoxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds.
摘要翻译： 提供了保护植物免受害虫害虫的组合物和方法。 特别地，提供了编码昆虫原毒素的核酸序列，其被修饰为包含对植物蛋白酶或昆虫肠道蛋白酶敏感的至少一个蛋白水解活化位点。 通过蛋白酶在蛋白水解活化位点切割修饰的原毒素会产生活性昆虫毒素。 提供了使用修饰的昆虫原毒素核酸序列及其编码的多肽保护植物免受害虫的方法。 本发明的具体实施方案进一步提供改良的昆虫原毒素组合物和制剂，表达盒，以及转化的植物，植物细胞和种子。
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公开(公告)号：US20090317891A1
公开(公告)日：2009-12-24
申请号：US12512425
申请日：2009-07-30
申请人： Andre Abad , Ronald D. Flannagan , Rafael Hermann , Albert L. Lu , Billy F. McCutchen , James K. Presnail , Janet A. Rice , James F. Wong , Cao-Guo Yu
发明人： Andre Abad , Ronald D. Flannagan , Rafael Hermann , Albert L. Lu , Billy F. McCutchen , James K. Presnail , Janet A. Rice , James F. Wong , Cao-Guo Yu
CPC分类号： C12N15/8286 , C12N9/641 , Y02A40/162
摘要： Compositions and methods for protecting a plant from an insect pest are provided. In particular, nucleic acid sequences encoding insect protoxins modified to comprise at least one proteolytic activation site that is sensitive to a plant protease or an insect gut protease are provided. Cleavage of the modified protoxin at the proteolytic activation site by a protease produces an active insect toxin. Methods of using the modified insect protoxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds.
摘要翻译： 提供了保护植物免受害虫害虫的组合物和方法。 特别地，提供了编码昆虫原毒素的核酸序列，其被修饰为包含对植物蛋白酶或昆虫肠道蛋白酶敏感的至少一个蛋白水解活化位点。 通过蛋白酶在蛋白水解活化位点切割修饰的原毒素会产生活性昆虫毒素。 提供了使用修饰的昆虫原毒素核酸序列及其编码的多肽保护植物免受害虫的方法。 本发明的具体实施方案还提供了改良的昆虫原毒素组合物和制剂，表达盒，以及转化的植物，植物细胞和种子。
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10.发明授权Isolated nucleic acid molecules encoding orally active androctonus amoreuxi pesticidal biopeptides 有权分离编码口服活性的芦荟杀虫生物肽的核酸分子公开(公告)号：US07414173B1
公开(公告)日：2008-08-19
申请号：US10617978
申请日：2003-07-11
IPC分类号： A01H5/00 , A01H5/10 , C12N15/82 , C12N15/12 , C07K14/435
CPC分类号： C12N15/8286 , C07K14/43522 , Y02A40/162
摘要： The present invention provides compositions and methods for isolated nucleic acid molecules encoding orally active Androctonus amoreuxi pesticidal polypeptides. Compositions include novel nucleotide sequences encoding Androctonus amoreuxi pesticidal polypeptides, and biologically active variants thereof. Further provided are methods for modulating the pesticide resistance of plants using the nucleotide sequences disclosed herein. One method comprises stably transforming into the genome of a plant cell a nucleotide sequence of the present invention operably linked to a heterologous promoter and regenerating a stably transformed plant that expresses the nucleotide sequence. An additional method comprises incorporating a nucleotide sequence of the present invention operably linked to a heterologous promoter into a microorganism and applying said microorganism to the environment of a plant.
摘要翻译： 本发明提供了编码口服活性黄芪杀虫多肽的分离的核酸分子的组合物和方法。 组合物包括编码Androctonus amoreuxi杀虫多肽的新型核苷酸序列及其生物活性变体。 还提供了使用本文公开的核苷酸序列调节植物的农药抗性的方法。 一种方法包括稳定地转化到植物细胞的基因组中，本发明的核苷酸序列与异源启动子可操作地连接并再生表达核苷酸序列的稳定转化的植物。 另外的方法包括将可操作地连接到异源启动子的本发明的核苷酸序列并入微生物并将所述微生物施用于植物的环境。
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