公开(公告)号：US20190250127A1

公开(公告)日：2019-08-15

申请号：US16336871

申请日：2017-09-26

Applicant: ARIZONA BOARD OF REGENTS on behalf of ARIZONA STATE UNIVERSITY

Inventor： Peiming ZHANG ,  Stuart LINDSAY

IPC: G01N27/447 ,  G01N33/487

CPC classification number: G01N27/44791 ,  C12Q1/6869 ,  G01N27/447 ,  G01N33/48721 ,  C12Q2521/101 ,  C12Q2563/119 ,  C12Q2563/149 ,  C12Q2565/607 ,  C12Q2565/631

Abstract: Apparatus and means by which a polysaccharide or other heterogeneous polymer is concatenated with a nucleic acid polymer that is captured by a primer on a polymerase tethered to a bead trapped by a nanopore. The translocation of the nanopore by the polysaccharide or other heterogeneous polymer is then controlled by the speed at which the polymerase releases newly synthesized nucleic acid or slows the motion of the nucleic acid as it is pulled on by an electrophoretic field.

公开(公告)号：US20190195884A1

公开(公告)日：2019-06-27

申请号：US16168081

申请日：2018-10-23

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Stuart LINDSAY ,  Peiming ZHANG ,  Yanan ZHAO

IPC: G01N33/68 ,  G01N33/487

CPC classification number: G01N33/6821 ,  G01N33/48721

Abstract: Embodiments of the present disclosure relate to amino acid, modified amino acid, peptide and protein identification and sequencing, by means of, for example, electronic detection of individual amino acids or small peptides.

公开(公告)号：US20150211059A1

公开(公告)日：2015-07-30

申请号：US14577715

申请日：2014-12-19

Applicant: ARIZONA BOARD OF REGENTS on behalf of ARIZONA STATE UNIVERSITY

Inventor： Kevin REINHART ,  Stuart LINDSAY ,  Peiming ZHANG

IPC: C12Q1/68 ,  G01N27/447

CPC classification number: C07D235/24 ,  C12Q1/6869 ,  G01N27/447 ,  G01N27/44791 ,  G01N33/48721 ,  C12Q2565/631

Abstract: The present invention is directed to systems, devices and methods for identifying biopolymers, such as strands of DNA, as they pass through a constriction such as a carbon nanotube nanopore. More particularly, the invention is directed to such systems, devices and methods in which a newly translocated portion of the iopolymer forms a temporary electrical circuit between the nanotube nanopore and a second electrode, which may also be a nanotube. Further, the invention is directed to such systems, devices and methods in which the constriction is provided with a transnaionilized unit which, together with a newly translocated portion of the biopolymer, forms a temporary electrical circuit that can be used to characterize the portion of the biopolymer.

Abstract translation: 本发明涉及用于识别生物聚合物的系统，装置和方法，例如当DNA通过诸如碳纳米管纳米孔的收缩体时，DNA的链。 更具体地说，本发明涉及这样的系统，装置和方法，其中聚合物的新易位部分在纳米管纳米孔和也可以是纳米管的第二电极之间形成临时电路。 此外，本发明涉及这样的系统，装置和方法，其中收缩部设置有横穿电泳单元，其与生物聚合物的新易位部分一起形成临时电路，其可用于表征部分 生物聚合物。

公开(公告)号：US20250001384A1

公开(公告)日：2025-01-02

申请号：US18732594

申请日：2024-06-03

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Quan QING ,  Ching-wei TSAO ,  Peiming ZHANG

IPC: B01J19/00 ,  C07K1/04

Abstract: The present disclosure provides an apparatus for synthesizing a biopolymer, a method for preparing an apparatus for synthesizing a biopolymer, and a method of synthesizing a biopolymer.

公开(公告)号：US20170168039A1

公开(公告)日：2017-06-15

申请号：US15375901

申请日：2016-12-12

Applicant: ARIZONA BOARD OF REGENTS on behalf of ARIZONA STATE UNIVERSITY

Inventor： Stuart LINDSAY ,  Peiming ZHANG ,  Yanan ZHAO

IPC: G01N33/487 ,  G01N27/447

CPC classification number: G01N33/48721

Abstract: A single molecule sensing or detecting device includes a first electrode and a second electrode separated from the first electrode by a gap. The first electrode and the second electrode have an opening formed therethrough. At least one of the first electrode and the second electrode is functionalized with a recognition molecule. The recognition molecule has an effective length L1 and is configured to selectively bind to a target molecule having an effective length L2. The size of the gap is configured to be greater than L2, but less than or equal to the sum of L1 and L2.

公开(公告)号：US20170067902A1

公开(公告)日：2017-03-09

申请号：US15119549

申请日：2015-02-18

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Peiming ZHANG ,  Stuart LINDSAY ,  Subhadip SENAPATI ,  Sudipta BISWAS

IPC: G01N33/58 ,  G01Q60/38 ,  C12Q1/68 ,  C07D495/04

CPC classification number: G01N33/587 ,  C07C247/04 ,  C07D495/04 ,  C12Q1/6816 ,  C12Q1/6834 ,  G01N33/58 ,  G01Q60/38 ,  C12Q2563/131

Abstract: Embodiments of the present disclosure provide bisbiotin ligands and related conjugates and methods. The bisbiotin ligands, combined with streptavidin, can be used in the separation, labelling, targeting, and immobilization of biomolecules.

Abstract translation: 本公开的实施方案提供双生物素配体和相关的缀合物和方法。 结合链霉抗生物素蛋白的双生物素配体可用于分离，标记，靶向和固定生物分子。

公开(公告)号：US20170038369A1

公开(公告)日：2017-02-09

申请号：US15303960

申请日：2015-04-16

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Stuart LINDSAY ,  Peiming ZHANG ,  Pei PANG

IPC: G01N33/543 ,  G01N33/487 ,  B01L3/00

CPC classification number: G01N33/5438 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2200/10 ,  B01L2300/0645 ,  B01L2300/0861 ,  B01L2300/0887 ,  G01N33/48721

Abstract: A sensing device is provided that includes a tunnel junction created by forming a hole in a layered tunnel junction (for example). A chemically, well-defined surface may be formed by coupling affinity reagents to the electrodes, which, by these means, the surface may be configured to be selective for a particular analyte.

Abstract translation: 提供了一种感测装置，其包括通过在分层隧道结（例如）中形成孔而产生的隧道结。 可以通过将亲和试剂偶联到电极来形成化学上明确定义的表面，通过这些方式，表面可被配置为对特定分析物具有选择性。

公开(公告)号：US20160097759A1

公开(公告)日：2016-04-07

申请号：US14851933

申请日：2015-09-11

Applicant: ARIZONA BOARD OF REGENTS on behalf of ARIZONA STATE UNIVERSITY

Inventor： Stuart LINDSAY ,  Shuai CHANG ,  Jin HE ,  Peiming ZHANG ,  Shuo HUANG

IPC: G01N33/487 ,  G01N27/327 ,  C12Q1/68

CPC classification number: G01N33/48721 ,  C12Q1/6869 ,  G01N27/3275 ,  Y10T436/143333

Abstract: The invention includes compositions, devices, and methods for analyzing a polymer and/or polymer unit. The polymer may be a homo or hetero-polymer such as DNA, RNA, a polysaccharide, or a peptide. The device includes electrodes that form a tunnel gap through which the polymer can pass. The electrodes are functionalized with a reagent attached thereto, and the reagent is capable of forming a transient bond to a polymer unit. When the transient bond forms between the reagent and the unit, a detectable signal is generated and used to analyze the polymer.

Abstract translation: 本发明包括用于分析聚合物和/或聚合物单元的组合物，装置和方法。 聚合物可以是同质或异聚合物，例如DNA，RNA，多糖或肽。 该装置包括形成隧道间隙的电极，聚合物可通过该间隙通过。 电极用附着于其上的试剂进行功能化，并且试剂能够形成与聚合物单元的暂时结合。 当试剂和单元之间形成瞬态键时，产生可检测的信号并用于分析聚合物。