公开(公告)号：US09023632B2

公开(公告)日：2015-05-05

申请号：US14150206

申请日：2014-01-08

Applicant: BASF SE

Inventor： Hartwig Schröder ,  Stefan Haefner ,  Gregory Von Abendroth ,  Rajan Hollmann ,  Aline Raddatz ,  Hansgeorg Ernst ,  Hans Gurski

IPC: C12P7/46 ,  C12P7/44 ,  C12N1/32 ,  C12N9/10 ,  C12R1/01

CPC classification number: C12P7/46 ,  C12N1/32 ,  C12N9/1029 ,  C12R1/01 ,  C12Y203/01054

Abstract: The present invention relates to bacterial strains, capable of utilizing glycerol as a carbon source for the fermentative production of succinic acid, wherein said strains are genetically modified so that they comprise a deregulation of their endogenous pyruvate-formate-lyase enzyme activity, as well as to methods of producing organic acids, in particular succinic acid, by making use of such microorganism. The present invention also relates to the downstream processing of the produced organic acids by cation exchange chromatography.

Abstract translation: 本发明涉及能够利用甘油作为发酵生产琥珀酸的碳源的细菌菌株，其中所述菌株被遗传修饰，使得它们包含其内源性丙酮酸 - 甲酸裂解酶活性的失调，以及 涉及通过利用这种微生物生产有机酸，特别是琥珀酸的方法。 本发明还涉及通过阳离子交换层析对所生产的有机酸的下游加工。