公开(公告)号：US07498413B2

公开(公告)日：2009-03-03

申请号：US11619645

申请日：2007-01-04

申请人： Mathias L. Muller ,  Carl R. Simmons ,  Nasser Yalpani

发明人： Mathias L. Muller ,  Carl R. Simmons ,  Nasser Yalpani

IPC分类号： C07K14/00 ,  C07K14/415 ,  C07K17/00

CPC分类号： C12N9/22 ,  C12N15/8282

摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include novel amino acid sequences, and variants and fragments thereof, for antipathogenic polypeptides that were isolated from maize. Nucleic acid molecules comprising nucleotide sequences that encode the antipathogenic polypeptides of the embodiments are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant a DNA construct comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the embodiments. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the embodiments in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the embodiments, or variant or fragment thereof, are also disclosed.

摘要翻译： 提供了用于保护植物免受病原体，特别是真菌病原体的组合物和方法。 组合物包括从玉米分离的抗病原体多肽的新型氨基酸序列及其变体和片段。 还提供了包含编码实施方案的抗病原多肽的核苷酸序列的核酸分子。 还提供使用本文公开的核苷酸序列在植物中诱导病原体抗性的方法。 该方法包括向植物中引入包含可操作地连接到编码实施方案的抗病原体多肽的核苷酸序列的启动子的DNA构建体。 还提供包含抗病原体多肽或包含实施方案的核酸与载体组合的转化微生物的组合物，以及使用这些组合物保护植物免受病原体的方法。 还公开了包含编码实施方案的抗病原体多肽的核苷酸序列或其变体或片段的转化植物，植物细胞，种子和微生物。

公开(公告)号：US20090055966A1

公开(公告)日：2009-02-26

申请号：US12197389

申请日：2008-08-25

申请人： Virginia C. Crane ,  Jeanne Sandahl ,  Carl R. Simmons

发明人： Virginia C. Crane ,  Jeanne Sandahl ,  Carl R. Simmons

IPC分类号： C12N15/82 ,  C07H21/04 ,  C12N15/64 ,  C12N5/04 ,  C12N5/10 ,  A01H5/00

CPC分类号： C12N15/8233 ,  C12N15/8234 ,  C12N15/8235

摘要： The present disclosure provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a tissue-preferred maize promoter. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequence disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the tissue-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.

摘要翻译： 本公开提供了用于调节植物中异源核苷酸序列表达的组合物和方法。 组合物包括用于组织优选的玉米启动子的新的核苷酸序列。 提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。 该方法包括将与本发明的组织优选启动子可操作地连接的核苷酸序列稳定地并入植物细胞的基因组中，并再生表达核苷酸序列的稳定转化的植物。

公开(公告)号：US07145060B2

公开(公告)日：2006-12-05

申请号：US10389432

申请日：2003-03-14

申请人： Mathias L. Muller ,  Thom True ,  Carl R. Simmons ,  Nasser Yalpani

发明人： Mathias L. Muller ,  Thom True ,  Carl R. Simmons ,  Nasser Yalpani

IPC分类号： A01H5/00 ,  C12N15/82 ,  C12N15/56

CPC分类号： C12Y302/01014 ,  C12N9/2442 ,  C12N15/8282 ,  C12N15/8286 ,  Y02A40/162

摘要： The present invention provides a nucleic acid encoding a chitinase and methods for it to enhance resistance of plants to fungal infections.

摘要翻译： 本发明提供编码几丁质酶的核酸及其增强植物对真菌感染的抗性的方法。

公开(公告)号：US06879916B2

公开(公告)日：2005-04-12

申请号：US10101080

申请日：2002-05-09

申请人： Ramgopal Nadimpalli ,  Carl R. Simmons

发明人： Ramgopal Nadimpalli ,  Carl R. Simmons

IPC分类号： G06F19/22 ,  G06F19/00 ,  C12Q1/68 ,  G01N33/48

CPC分类号： G06F19/22 ,  Y10S707/99931

摘要： The invention provides isolated prolifera nucleic acids and their encoded proteins. The present invention provides methods and compositions relating to altering prolifera levels in plants. The invention further provides recombinant expression cassettes, host cells, transgenic plants, and antibody compositions.

摘要翻译： 本发明提供了分离的增殖核酸及其编码的蛋白质。 本发明提供了关于改变植物中增殖物水平的方法和组合物。 本发明还提供了重组表达盒，宿主细胞，转基因植物和抗体组合物。

公开(公告)号：US06720480B2

公开(公告)日：2004-04-13

申请号：US10039836

申请日：2001-10-23

申请人： Virginia C. Crane ,  Carl R. Simmons

发明人： Virginia C. Crane ,  Carl R. Simmons

IPC分类号： A01H500

CPC分类号： A01H4/005 ,  C12N15/8237 ,  C12N15/8238 ,  C12N15/8239 ,  C12N15/8279

摘要： Methods and compositions for modulating development and defense response are provided. Nucleotide sequences encoding a maize proteinase inhibitor-like protein is provided. Nucleotide sequences comprising the proteinase inhibitor-like promoter are also provided. Methods of using the promoter to express nucleic acid sequences of interest are provided. The sequences can be used in expression cassettes for modulating development, developmental pathways, and the plant defense response. Plants, plant cells, tissues, and seed transformed with the proteinase inhibitor-like gene or its promoter are also provided.

摘要翻译： 提供了调节发育和防御反应的方法和组合物。 提供编码玉米蛋白酶抑制剂样蛋白质的核苷酸序列。 还提供了包含蛋白酶抑制剂样启动子的核苷酸序列。 提供了使用启动子来表达感兴趣的核酸序列的方法。 序列可用于表达盒中用于调节发育，发育途径和植物防御反应。 还提供了用蛋白酶抑制剂样基因或其启动子转化的植物，植物细胞，组织和种子。

公开(公告)号：US06433249B1

公开(公告)日：2002-08-13

申请号：US09431470

申请日：1999-11-01

申请人： Jon Duvick ,  Carl R. Simmons ,  Oswald R. Crasta ,  Otto Folkerts

发明人： Jon Duvick ,  Carl R. Simmons ,  Oswald R. Crasta ,  Otto Folkerts

IPC分类号： A01H100

CPC分类号： C12N15/8286 ,  C12N15/8282 ,  Y02A40/162

摘要： Methods for the enhancement of plant disease resistance are provided. The methods comprise transforming said plant with a gene encoding &bgr;-glucosidase and increasing the expression said gene above wild-type levels. Transformed plants, plant cells and seeds are provided. Disease resistant transformed plants, plant cells and seeds are also provided.

摘要翻译： 提供了提高植物抗病性的方法。 所述方法包括用编码β-葡糖苷酶的基因转化所述植物，并将所述基因的表达增加到野生型水平以上。 提供了转化的植物，植物细胞和种子。 还提供了抗病性转化的植物，植物细胞和种子。

公开(公告)号：US06211433B1

公开(公告)日：2001-04-03

申请号：US09350268

申请日：1999-07-06

申请人： Carl R. Simmons ,  Guo-Hua Miao

发明人： Carl R. Simmons ,  Guo-Hua Miao

IPC分类号： C12N1509

CPC分类号： C07K14/415 ,  C12N15/8279 ,  C12N15/8282

摘要： Compositions and methods for enhancing disease resistance in plants are provided. The method involves modulating the activity of the Mlo3 sequences in the plant. Mlo3 sequences are provided that can be manipulated to enhance pathogen resistance in modified plants. Transformed plants, plant cells, tissues, and seed are also provided having enhanced disease resistance.

摘要翻译： 提供了增强植物抗病性的组合物和方法。 该方法涉及调节植物中Mlo3序列的活性。 提供Mlo3序列，其可以被操纵以增强修饰植物中的病原体抗性。 还提供了具有增强的抗病性的转化植物，植物细胞，组织和种子。

公开(公告)号：US08962916B2

公开(公告)日：2015-02-24

申请号：US13329511

申请日：2011-12-19

申请人： Scott Diehn ,  Albert L. Lu ,  Carl R. Simmons

发明人： Scott Diehn ,  Albert L. Lu ,  Carl R. Simmons

IPC分类号： C12N15/82 ,  A01H5/00 ,  C12N15/00 ,  C12N5/04 ,  C12N5/14 ,  C07H21/04

CPC分类号： C12N15/8226 ,  C12N15/8216 ,  C12N15/8227 ,  C12N15/8286 ,  Y02A40/162

摘要： The present disclosure provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a promoter. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequence disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.

摘要翻译： 本公开提供了用于调节植物中异源核苷酸序列表达的组合物和方法。 组合物包括用于启动子的新型核苷酸序列。 提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。 该方法包括将与本发明的启动子可操作地连接的核苷酸序列稳定地并入植物细胞的基因组中，并再生表达核苷酸序列的稳定转化的植物。

公开(公告)号：US08395022B2

公开(公告)日：2013-03-12

申请号：US12847534

申请日：2010-07-30

申请人： Scott Diehn ,  Albert L. Lu ,  Carl R. Simmons

发明人： Scott Diehn ,  Albert L. Lu ,  Carl R. Simmons

IPC分类号： C12N15/82 ,  C12N15/113 ,  C12N5/10

CPC分类号： C12N15/8222

摘要： The present disclosure provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a promoter. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequence disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.

公开(公告)号：US20120124699A1

公开(公告)日：2012-05-17

申请号：US13358040

申请日：2012-01-25

申请人： James English ,  Carl R. Simmons ,  Nasser Yalpani

发明人： James English ,  Carl R. Simmons ,  Nasser Yalpani

IPC分类号： C12N15/82 ,  A01H5/10 ,  A01H5/00 ,  C07H21/04 ,  C12N5/10

CPC分类号： C12N15/8279 ,  C07K14/415 ,  C12N15/8222 ,  C12N15/8239 ,  C12N15/8282 ,  C12N15/8285 ,  C12N15/8286 ,  Y02A40/162 ,  Y02A40/164

摘要： Methods and compositions for modulating development and defense responses are provided. Nucleotide sequences encoding plant defense proteins are provided. The sequences can be used in expression cassettes for modulating development, developmental pathways, and defense responses. Transformed plants, plant cells, tissues, and seed are also provided.

摘要翻译： 提供了调节发展和防御反应的方法和组成。 提供编码植物防御蛋白的核苷酸序列。 序列可用于表达盒用于调节发育，发育途径和防御反应。 还提供了转化的植物，植物细胞，组织和种子。