公开(公告)号：US20170114341A1

公开(公告)日：2017-04-27

申请号：US15315608

申请日：2015-06-08

Applicant: Curt W. BRADSHAW ,  Sukumar SAKAMURI ,  Laxman ELTEPU ,  Son LAM ,  Dingguo LIU ,  Bryan MEADE ,  Giuseppe Dello IACONO ,  Joseph STOCK ,  Bin LIU ,  Solstice Biologics, Ltd.

Inventor： Curt W. BRADSHAW ,  Sukumar SAKAMURI ,  Laxman ELTEPU ,  Son LAM ,  Dingguo LIU ,  Bryan MEADE ,  Giuseppe Dello IACONO ,  Joseph STOCK ,  Bin LIU

IPC: C12N15/113 ,  C07H19/20 ,  C07H21/02 ,  C07H19/10 ,  C12N15/85 ,  C07H21/04

CPC classification number: C12N15/113 ,  C07H19/10 ,  C07H19/20 ,  C07H21/02 ,  C07H21/04 ,  C12N15/85 ,  C12N2310/14 ,  C12N2310/31 ,  C12N2310/32 ,  C12N2310/346 ,  C12N2310/3513 ,  C12N2310/3515 ,  C12N2330/30

Abstract: The invention features a hybridized polynucleotide construct containing a passenger strand, a guide strand loadable into a RISC complex, and (i) a 3′-terminal or an internucleotide non-bioreversible group in the guide strand; or (ii) a 5′-terminal, a 3′-terminal, or an internucleotide non-bioreversible group in the passenger strand, and a 5′-terminal, a 3′-terminal, or an internucleotide disulfide bioreversible group in the guide strand or the passenger strand. The invention also features methods of delivering a polynucleotide to a cell using the hybridized polynucleotide construct. The invention further features methods of reducing the expression of a polypeptide in a cell using the hybridized polynucleotide construct.