公开(公告)号：US08273867B2

公开(公告)日：2012-09-25

申请号：US12278739

申请日：2007-02-09

Applicant: Steven F. Dowdy ,  Jehangir S. Wadia ,  Bryan Meade ,  Akiko Eguchi

Inventor： Steven F. Dowdy ,  Jehangir S. Wadia ,  Bryan Meade ,  Akiko Eguchi

IPC: C12N15/11 ,  C07H21/02

CPC classification number: C12N15/111 ,  C07K2319/10 ,  C07K2319/85 ,  C12N2310/14 ,  C12N2320/32

Abstract: The disclosure provides fusion polypeptides and constructs useful in delivering anionically charged nucleic acid molecules including diagnostics and therapeutics to a cell or subject. The fusion constructs include a protein transduction domain and a nucleic acid binding domain, or a protein transduction domain and a nucleic acid that is coated with one or more nucleic acid binding domains sufficient to neutralize an anionic charge on the nucleic acid. Also provided are methods of treating disease and disorders such as cell proliferative disorders.

Abstract translation: 本公开提供了融合多肽和构建体，其可用于将阴离子电荷核酸分子（包括诊断和治疗）递送至细胞或受试者。 融合构建体包括蛋白质转导结构域和核酸结合结构域，或蛋白质转导结构域和被一个或多个核酸结合域包被的核酸，所述核酸结合域足以中和核酸上的阴离子电荷。 还提供了治疗疾病和病症如细胞增殖性疾病的方法。

公开(公告)号：US20170114341A1

公开(公告)日：2017-04-27

申请号：US15315608

申请日：2015-06-08

Applicant: Curt W. BRADSHAW ,  Sukumar SAKAMURI ,  Laxman ELTEPU ,  Son LAM ,  Dingguo LIU ,  Bryan MEADE ,  Giuseppe Dello IACONO ,  Joseph STOCK ,  Bin LIU ,  Solstice Biologics, Ltd.

Inventor： Curt W. BRADSHAW ,  Sukumar SAKAMURI ,  Laxman ELTEPU ,  Son LAM ,  Dingguo LIU ,  Bryan MEADE ,  Giuseppe Dello IACONO ,  Joseph STOCK ,  Bin LIU

IPC: C12N15/113 ,  C07H19/20 ,  C07H21/02 ,  C07H19/10 ,  C12N15/85 ,  C07H21/04

CPC classification number: C12N15/113 ,  C07H19/10 ,  C07H19/20 ,  C07H21/02 ,  C07H21/04 ,  C12N15/85 ,  C12N2310/14 ,  C12N2310/31 ,  C12N2310/32 ,  C12N2310/346 ,  C12N2310/3513 ,  C12N2310/3515 ,  C12N2330/30

Abstract: The invention features a hybridized polynucleotide construct containing a passenger strand, a guide strand loadable into a RISC complex, and (i) a 3′-terminal or an internucleotide non-bioreversible group in the guide strand; or (ii) a 5′-terminal, a 3′-terminal, or an internucleotide non-bioreversible group in the passenger strand, and a 5′-terminal, a 3′-terminal, or an internucleotide disulfide bioreversible group in the guide strand or the passenger strand. The invention also features methods of delivering a polynucleotide to a cell using the hybridized polynucleotide construct. The invention further features methods of reducing the expression of a polypeptide in a cell using the hybridized polynucleotide construct.

公开(公告)号：US20090093026A1

公开(公告)日：2009-04-09

申请号：US12278739

申请日：2007-02-09

Applicant: Steven F. Dowdy ,  Jehangir S. Wadia ,  Bryan Meade ,  Akiko Eguchi

Inventor： Steven F. Dowdy ,  Jehangir S. Wadia ,  Bryan Meade ,  Akiko Eguchi

IPC: C12P21/02 ,  C07K14/00 ,  A61K38/16 ,  C07K14/03 ,  C12N15/09 ,  C12N5/06 ,  C07H21/00 ,  C12N15/63 ,  C12N5/10

CPC classification number: C12N15/111 ,  C07K2319/10 ,  C07K2319/85 ,  C12N2310/14 ,  C12N2320/32

Abstract: The disclosure provides fusion polypeptides and constructs useful in delivering anionically charged nucleic acid molecules including diagnostics and therapeutics to a cell or subject. The fusion constructs include a protein transduction domain and a nucleic acid binding domain, or a protein transduction domain and a nucleic acid that is coated with one or more nucleic acid binding domains sufficient to neutralize an anionic charge on the nucleic acid. Also provided are methods of treating disease and disorders such as cell proliferative disorders.

Abstract translation: 本公开提供了融合多肽和构建体，其可用于将阴离子电荷核酸分子（包括诊断和治疗）递送至细胞或受试者。 融合构建体包括蛋白质转导结构域和核酸结合结构域，或蛋白质转导结构域和被一个或多个核酸结合域包被的核酸，所述核酸结合域足以中和核酸上的阴离子电荷。 还提供了治疗疾病和病症如细胞增殖性疾病的方法。