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11.发明申请公开(公告)号：US20200010863A1
公开(公告)日：2020-01-09
申请号：US16503301
申请日：2019-07-03
发明人： Wenbo LIU , Min XU , Chen YANG , Howard CHOU , Xiucai LIU
摘要： The invention relates to a long-chain dibasic acid with low content of monobasic acid impurity and a production method thereof, in particular to the preparation of a long-chain dibasic acid producing strain by means of directed evolution and homologous recombination, and to the production of a long-chain dibasic acid with low content of monobasic acid impurity by fermentation of said strain. The invention relates to a mutated CYP52A12 gene, homologous gene or variant thereof, which, relative to GenBank Accession Number AY230498 and taking the first base upstream of the start codon ATG as −1, comprises a mutation. The invention relates to a strain comprising said mutated CYP52A12 gene, homologous gene or variant thereof wherein when the strain is fermented to produce a long-chain dibasic acid, the content of monobasic acid impurity in the fermentation product is significantly reduced.
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12.发明申请公开(公告)号：US20200010859A1
公开(公告)日：2020-01-09
申请号：US16503309
申请日：2019-07-03
发明人： Wenbo LIU , Min XU , Chen YANG , Howard CHOU , Xiucai LIU
摘要： The present invention relates to a long-chain dibasic acid with low content of long-chain dibasic acid impurity of shorter carbon chain, to the preparation of a long-chain dibasic acid producing strain by directed evolution of POX gene and homologous recombination, and to the production of a long-chain dibasic acid with low content of long-chain dibasic acid impurity of shorter carbon chain by using the strain. The present invention also relates to a strain containing a mutated promoter, wherein, when a long-chain dibasic acid is produced by fermentation of this strain, the content of the acid impurity of shorter carbon chain in the fermentation product is significantly reduced.
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13.发明申请公开(公告)号：US20200010855A1
公开(公告)日：2020-01-09
申请号：US16503304
申请日：2019-07-03
发明人： Wenbo LIU , Min XU , Chen YANG , Howard CHOU , Xiucai LIU
摘要： The present invention relates to a long-chain dibasic acid with low content of hydroxyl acid impurity and a production method thereof, in particular to a method for producing a long-chain dibasic acid with low content of hydroxyl acid impurity by fermenting a long-chain dibasic acid producing strain prepared by homologous recombination method. The present invention relates to a recombinant long-chain dibasic acid producing microorganism, having increased alcohol dehydrogenase activity and optionally decreased acetyl-CoA oxidase activity. The present invention also relates to a method of producing a long-chain dibasic acid by the recombinant long-chain dibasic acid producing microorganism and use thereof.
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公开(公告)号：US20230051716A1
公开(公告)日：2023-02-16
申请号：US17758892
申请日：2020-01-15
发明人： Chaoxu SUN , Xiucai LIU
摘要： The present application discloses a polyamide 5X fiber, wherein raw materials for producing the polyamide 5X fiber comprise at least 1,5-pentane diamine and a long carbon chain diacid; or comprises a polyamide 5X obtained by the polymerization of 1,5-pentane diamine and a long carbon chain diacid as monomers. The 1,5-pentane diamine or the long carbon chain diacid are produced from bio-based raw materials by a fermentation process or an enzymatic conversion process; and the long carbon chain diacid is at least one of C6-20 aliphatic long carbon chain diacids. The long carbon chain diacid is at least one of C6-20 aliphatic long carbon chain diacids. The polyamide 5X fiber includes pre-oriented yarns, drawn textured yarns, fully drawn yarns, staple fibers, industrial yarns, continuous bulked filaments and monofilaments, preferably pre-oriented yarns, drawn textured yarns and/or fully drawn yarns. The raw materials used for producing the polyamide 5X resin are prepared by biological processes, and are green materials. The polyamide 5X fiber has good mechanical properties, dimensional stability, softness, quick-drying performance and dyeing properties.
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公开(公告)号：US20220389620A1
公开(公告)日：2022-12-08
申请号：US17774937
申请日：2020-01-15
发明人： Chaoxu SUN , Wanzhong CHEN , Shuyuan CHEN , Zhiguo SHANG , Xiucai LIU
IPC分类号： D01F6/60 , D01F1/10 , D01D5/08 , D01D5/088 , D01D5/16 , D01D10/02 , C08G69/28 , C08G69/32 , C08K5/098 , C08K3/16
摘要： Disclosed in the present invention is a polyamide 5X industrial yarn. The polyamide 5X industrial yarn has a heat-resistant break strength retention rate of 90% or more after being treated at 180° C. for 4 hrs; a heat-resistant break strength retention rate of 90% or more after being treated at 230° C. for 30 mins; and a dry heat shrinkage of 8.0% or less. The polyamide 5X industrial yarn is widely used in the fields of sewing threads, tire cords, air bag yarns, release cloth, krama, canvas, safety belts, ropes, fishing nets, industrial filter cloth, conveyor belts, parachutes, tents, bags and suitcases.
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公开(公告)号：US20220380816A1
公开(公告)日：2022-12-01
申请号：US17814972
申请日：2022-07-26
发明人： Wenbo LIU , Min XU , Chen YANG , Howard CHOU , Xiucai LIU
摘要： The present invention relates to a long-chain dibasic acid with low content of long-chain dibasic acid impurity of shorter carbon chain, to the preparation of a long-chain dibasic acid producing strain by directed evolution of POX gene and homologous recombination, and to the production of a long-chain dibasic acid with low content of long-chain dibasic acid impurity of shorter carbon chain by using the strain. The present invention also relates to a strain containing a mutated promoter, wherein, when a long-chain dibasic acid is produced by fermentation of this strain, the content of the acid impurity of shorter carbon chain in the fermentation product is significantly reduced.
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公开(公告)号：US20210180032A9
公开(公告)日：2021-06-17
申请号：US16229084
申请日：2018-12-21
发明人： Wenbo LIU , Min XU , Howard CHOU , Xiucai LIU
摘要： The invention relates to the directed evolution of CYP52A12 gene and the use thereof for the production of a dicarboxylic acid. In particular, it relates to a method of preparing a long chain dicarboxylic acid producing strain by using directed evolution and homologous recombination, a strain obtained by the method that is capable of producing a long chain dicarboxylic acid under an acidic condition and the use thereof. In particular, the invention relates to a method of preparing a long chain dicarboxylic acid producing strain by directed evolution of CYP52A12 gene and homologous recombination, a strain obtained by the method that is capable of producing a long chain dicarboxylic acid under an acidic condition and the use thereof. By directed evolution of CYP52A12 gene, one strain which has a base mutation at the promoter region of said gene and is capable of producing a long chain dicarboxylic acid under an acidic condition in a shortened fermentation time is screened out in the invention.
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公开(公告)号：US20210164003A1
公开(公告)日：2021-06-03
申请号：US16768336
申请日：2017-11-29
发明人： Howard CHOU , Ling CHEN , Xiucai LIU
摘要： Provided microorganisms genetically modified to overexpress an imine/enamine deaminase to enhance the production of lysine and lysine derivatives by the microorganism. Also provided a method of generating such microorganism, and methods of producing lysine and lysine derivatives using the genetically modified microorganisms.
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公开(公告)号：US20210024446A1
公开(公告)日：2021-01-28
申请号：US17039165
申请日：2020-09-30
发明人： Bingbing QIN , Yufeng YANG , Xiucai LIU
摘要： The present disclosure provides method for isolating a long chain dicarboxylic acid such as a substantially pure or pure long chain dicarboxylic acid from a fermentation broth containing microbial cells.
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20.发明申请公开(公告)号：US20200010862A1
公开(公告)日：2020-01-09
申请号：US16503268
申请日：2019-07-03
发明人： Wenbo LIU , Min XU , Chen YANG , Howard CHOU , Xiucai LIU
摘要： The invention relates to a long-chain dibasic acid with low content of fatty acid impurity and a method of producing it, in particular to the preparation of a long-chain dibasic acid producing strain by using directed evolution and homologous recombination, and to the fermentation production of a long-chain dibasic acid with low content of fatty acid impurity by using said strain. The invention relates to an isolated mutated CPR-b gene, homologous gene or variant thereof, relative to the GenBank Accession Number AY823228, taking the first base upstream of the start codon ATG as −1, comprising a base mutation −322G>A, and taking the first base downstream of the stop codon TAG as 1, comprising mutations 3TUTR.19C>T and 3′UTR.76_77insT. The invention also relates to a strain containing said mutated CPR-b gene, homologous gene or variant thereof, wherein, when the strain is used to produce a long-chain dibasic acid by fermentation, the content of fatty acid impurity in the fermentation product is significantly decreased.
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