STRAIN FOR PRODUCING LONG-CHAIN DICARBOXYLIC ACIDS AND FERMENTATION METHOD THEREFOR

    公开(公告)号:US20230203431A1

    公开(公告)日:2023-06-29

    申请号:US17769459

    申请日:2020-01-16

    IPC分类号: C12N1/16 C12P7/44

    摘要: Provided are a Candida tropicalis strain Am2525, with the preservation number thereof being CCTCC NO: M 2019419, and a method for producing long-chain dicarboxylic acids by means of fermenting the strain. The method for producing the long-chain dicarboxylic acids comprises preparing a seed solution by means of the Candida tropicalis strain Am2525 and producing the long-chain dicarboxylic acids via fermentation of the seed solution. Compared with the parent, the Candida tropicalis strain Am2525 has an enhanced resistance to the toxicity of a substrate decane, improves the productivity of long-chain dicarboxylic acids, reduces the cost of production, subsequent separation and purification are simple, and the fermentation production process is easy to implement on a large scale.

    EXPRESSION OF KLEBSIELLA OXYTOCA POLYPEPTIDES INVOLVED IN LYSINE DECARBOXYLATION, AND METHODS AND APPLICATIONS THEREOF

    公开(公告)号:US20200157152A1

    公开(公告)日:2020-05-21

    申请号:US16738663

    申请日:2020-01-09

    摘要: One aspect provided herein relates to lysine decarboxylase polypeptides comprising mutants of SEQ ID NO: 2 (i.e., mutants of Klebsiella oxytoca (K. oxytoca) Ldc) and/or fragments thereof. In certain embodiments, the mutants or fragments thereof may have at least about 95% sequence identity with SEQ ID NO: 2. Another aspect provided herein relates to a DNA polynucleotide comprising one or more lysine decarboxylase nucleotide sequences of mutants of SEQ ID NO: 1 (i.e., mutants of K. oxytoca ldc), fragments thereof, or fragments of SEQ ID NO: 1 (i.e., fragments of K. oxytoca ldc). In certain embodiments, the DNA polynucleotide as disclosed herein may encode one or more lysine decarboxylase polypeptides provided herein. In certain embodiments, the DNA polynucleotide as disclosed herein may encode SEQ ID NO: 2, mutants, and/or fragments thereof. In certain embodiments, the lysine decarboxylase nucleotide sequences provided herein may have at least 95% sequence identity with SEQ ID NO: 1 or SEQ ID NO: 3. Another aspect provided herein relates to expression vectors comprising the DNA polynucleotides described herein used for production of a lysine-derived product. Other aspects provided herein include transformants, mutant host cells, methods for the production of lysine decarboxylases, and methods for the production of a lysine-derived product.

    POLYAMIDE SEA-ISLAND FIBER, PREPARATION METHOD THEREFOR, AND USE THEREOF

    公开(公告)号:US20220389623A1

    公开(公告)日:2022-12-08

    申请号:US17769779

    申请日:2020-01-15

    发明人: Chaoxu SUN Xiucai LIU

    摘要: The present invention relates to the technical field of polyamide materials, and specifically relates to a polyamide sea-island fiber and a process for producing the same and the use thereof. In the polyamide sea-island fiber, the island component is a polyamide resin selected from one of polyamide 56, polyamide 510, polyamide 511, polyamide 512, polyamide 513, polyamide 514, polyamide 515 and polyamide 516, preferably polyamide 56 or polyamide 510; the sea component is one of polyethylene, low-density polyethylene, polystyrene, water-soluble polyesters, polyesters and polyurethanes, preferably polyethylene, low-density polyethylene or water-soluble polyester. The polyamide sea-island fiber of the present invention has better mechanical properties, better softness, good dyeing properties, high grade of dyeing grey scale, high dye uptake, high dyeing depth and high color fastness.

    POLYAMIDE 56 FIBRE WITH LOW BOILING WATER SHRINKAGE RATE, PREPARATION METHOD THEREFOR, AND APPLICATION THEREOF

    公开(公告)号:US20220282404A1

    公开(公告)日:2022-09-08

    申请号:US17637093

    申请日:2020-01-15

    发明人: Chaoxu SUN Xiucai LIU

    摘要: The present invention relates to the technical field of polyamide materials, and provides a polyamide 56 pre-oriented yarn and a polyamide 56 draw textured yarn with a low shrinkage in boiling water, a process for producing the same and a use thereof. The raw materials for producing the polyamide 56 fiber comprise 1,5-pentane diamine and adipic acid, or a polyamide 56 resin obtained by polymerizing 1,5-pentane diamine monomer and adipic acid monomer; wherein the polyamide 56 fiber has a shrinkage in boiling water of 9% or less, wherein the polyamide 56 fiber comprises a polyamide 56 pre-oriented yarn with a low shrinkage in boiling water and a polyamide 56 draw textured yarn with a low shrinkage in boiling water. The polyamide 56 fiber has good mechanical properties, dyeing uniformity and dimensional stability. The process for producing the polyamide 56 fiber does not require modification to existing equipments for texturing polyamide fibers and thus reduces production costs.

    DIRECTED EVOLUTION OF CYP52A12 GENE AND ITS USE IN DICARBOXYLIC ACID PRODUCTION

    公开(公告)号:US20210340506A1

    公开(公告)日:2021-11-04

    申请号:US17373341

    申请日:2021-07-12

    IPC分类号: C12N9/02 C12N15/52

    摘要: The invention is directed to a method of preparing a long chain dicarboxylic acid producing strain by using directed evolution and homologous recombination, a strain obtained by this method that is capable of producing a long chain dicarboxylic acid under an acidic condition and the use of the strain. In particular, the invention is directed to a method of preparing a long chain dicarboxylic acid producing strain by using directed evolution of CYP52A12 gene and homologous recombination, a strain obtained by this method that is capable of producing a long chain dicarboxylic acid under an acidic condition and the use of the strain.