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20 条记录 (耗时 0.043 秒)

    Immunotargeting of Nonionic Surfactant Vesicles
    11.
    发明申请
    Immunotargeting of Nonionic Surfactant Vesicles 审中-公开
    非离子表面活性剂囊泡的免疫靶向

    公开(公告)号：US20070172520A1

    公开(公告)日：2007-07-26

    申请号：US11561639

    申请日：2006-11-20

    申请人： Michael VanAuker ,  Anna Plaas ,  Elizabeth Hood

    发明人： Michael VanAuker ,  Anna Plaas ,  Elizabeth Hood

    IPC分类号： A61K39/395 ,  A61K9/127

    CPC分类号： A61K39/00 ,  A61K9/1272 ,  A61K39/44 ,  A61K47/6913

    摘要： An immunoniosmes for targeted delivery of therapeutic agents to specific tissues in a host and methods of synthesis of those niosomes. An antibody molecule having specificity for a target antigen, such as a cell surface marker or other marker differentially expressed on a target cell, is covalently coupled to a functionalized membrane constituent. In a particular embodiment the functionalized membrane constituent is polyoxyethylene sorbitan monostearate functionalized with cyanuric chloride. The niosomes of this invention thus provide a composition that enhances internalization or retention of the bioactive agent of the niosome into the cytoplasm of the cells of the target tissue by providing a high degree of target specificity. Furthermore, the membrane vesicle enhances the life of the therapeutic agent by preventing its degradation in the extracellular environment, while exhibiting lower toxicity than can occur with some liposomes. The niosomes of the present invention are thus particularly useful as vehicles for the delivery of therapeutics to specific target cells.

    摘要翻译： 将治疗剂靶向递送到宿主中的特定组织的免疫组织和合成这些niosomes的方法。 对靶抗原（例如细胞表面标志物或靶细胞上差异表达的其他标记物）具有特异性的抗体分子与官能化的膜成分共价偶联。 在一个具体实施方案中，官能化膜组分是用氰尿酰氯官能化的聚氧乙烯脱水山梨醇单硬脂酸酯。 因此，本发明的niosomes提供了一种组合物，其通过提供高度的靶特异性来增强niosome的生物活性剂的内化或保留到靶组织的细胞的细胞质中。 此外，膜囊泡通过防止其在细胞外环境中的降解来增强治疗剂的寿命，同时表现出比一些脂质体可能发生的毒性更低的毒性。 因此，本发明的niosomes特别可用作将治疗剂递送到特定靶细胞的载体。

    Ultrasound enhancement of drug release across non-ionic surfactant membranes
    14.
    发明授权
    Ultrasound enhancement of drug release across non-ionic surfactant membranes 有权
    通过非离子表面活性剂膜超声增强药物释放

    公开(公告)号：US08435558B1

    公开(公告)日：2013-05-07

    申请号：US13185100

    申请日：2011-07-18

    申请人： Elizabeth Hood ,  Joel A. Strom ,  Michael VanAuker

    发明人： Elizabeth Hood ,  Joel A. Strom ,  Michael VanAuker

    IPC分类号： A61K9/127 ,  A61K9/48

    CPC分类号： A61K9/1272 ,  A61K9/0009 ,  A61K41/0028 ,  A61K49/0002 ,  A61K49/22

    摘要： A method of targeted drug delivery and imaging using nonionic surfactant vesicles (niosomes) in combination with ultrasound is presented. Niosomes have potential applications in targeted drug delivery and imaging because of their ability to encapsulate therapeutic agents and their enhanced uptake by physiological membranes. The niosomes may be administered to the subject via catheter. Ultrasound may be used to mediate delivery non-invasively by altering the niosome membrane structure. Niosomes composed of polyoxyethylene sorbitan monostearate (Tween 61), cholesterol, and dicetyl phosphate were synthesized via a thin film hydration technique and used for encapsulation studies. Carboxyfluorescein dye (CF) was used as a drug model to demonstrate delivery. The amount of dye in the niosomes, the concentration of the vesicles, and their mean particle size after each 5 minute incremental exposure to ultrasound was monitored. It was found that ultrasound at specific frequencies can reversibly permeabilize the lipid membrane of niosomes to allow the controlled release of a compound without destroying the niosome structure.

    摘要翻译： 提出了一种使用非离子表面活性剂囊泡（niosomes）与超声结合的靶向药物递送和成像方法。 Niosomes由于其能够包封治疗剂和增强生理膜吸收的能力，在靶向药物递送和成像中具有潜在应用。 可以通过导管向受试者施用niosomes。 超声波可用于通过改变niosome膜结构非侵入性地介导递送。 通过薄膜水合技术合成由聚氧乙烯脱水山梨醇单硬脂酸酯（吐温61），胆固醇和磷酸二鲸蜡酯组成的纳米粒子，用于封装研究。 使用羧基荧光素染料（CF）作为药物模型来证明递送。 监测niosomes中的染料量，囊泡浓度及每5分钟增加暴露于超声波后的平均粒径。 发现特定频率的超声波可以可逆地渗透niosomes的脂质膜，以允许化合物的受控释放而不破坏niosome结构。

    Commerical production of proteases in plants
    16.
    发明申请
    Commerical production of proteases in plants 审中-公开
    植物中商业化生产蛋白酶

    公开(公告)号：US20060015971A1

    公开(公告)日：2006-01-19

    申请号：US11226788

    申请日：2005-09-14

    申请人： John Howard ,  Elizabeth Hood

    发明人： John Howard ,  Elizabeth Hood

    IPC分类号： A01H1/00 ,  C12N15/82

    CPC分类号： C12N9/6427 ,  C12N15/8257

    摘要： Production of proteases in plants is set forth, whereby heterologous DNA encoding the protease is introduced into the plant and expression of the protein achieved. By such methods, expression is achieved in plants wherein the plant cell is not damaged, the protein can be recovered without contamination by other proteases, and can be expressed at levels such that commercial production of the enzyme is obtained. Expression levels can be at 0.1% of total soluble protein of the plant, or higher.

    摘要翻译： 阐述植物中蛋白酶的产生，由此将编码蛋白酶的异源DNA引入植物中并实现蛋白质的表达。 通过这样的方法，在植物细胞未受损害的植物中实现表达，可以在不受其它蛋白酶污染的情况下回收蛋白质，并且可以以获得酶的商业生产的水平来表达。 表达水平可以在植物总可溶性蛋白质的0.1％以上。

    Monocotyledonous seed expressing exo-1,4B-glucanase
    18.
    发明授权
    Monocotyledonous seed expressing exo-1,4B-glucanase 有权
    表达外切-14B-葡聚糖酶的单子叶种子

    公开(公告)号：US08558058B2

    公开(公告)日：2013-10-15

    申请号：US11219180

    申请日：2005-09-02

    申请人： Elizabeth Hood ,  John Howard

    发明人： Elizabeth Hood ,  John Howard

    IPC分类号： C12N15/82 ,  A01H5/00

    CPC分类号： C12N15/8242 ,  C12N15/8246 ,  C12N15/8257

    摘要： Expression of recombinant polysaccharide degrading enzymes in plants is described. In one embodiment, expression of the enzyme is preferentially directed to the seed of the plant. Expression may also be preferentially targeted to specific locations within the plant cell. Expression of cellulases in corn is shown. The result is the capacity to produce polysaccharide degrading enzymes in plants at commercially acceptable levels in a reliable manner. Methods of using same in production of ethanol is also described, including use of the plant-produced enzymes in the ethanol production process.

    摘要翻译： 描述了重组多糖降解酶在植物中的表达。 在一个实施方案中，酶的表达优先针对植物的种子。 表达也可以优先靶向植物细胞内的特定位置。 显示玉米纤维素酶的表达。 结果是以可靠的方式以商业上可接受的水平在植物中产生多糖降解酶的能力。 还描述了在乙醇生产中使用其的方法，包括在乙醇生产过程中使用植物生产的酶。

    Niosome-Hydrogel Drug Delivery
    20.
    发明申请
    Niosome-Hydrogel Drug Delivery 审中-公开
    Niosome水凝胶药物递送

    公开(公告)号：US20080050445A1

    公开(公告)日：2008-02-28

    申请号：US11737271

    申请日：2007-04-19

    申请人： Norma Alcantar ,  Kristina Dearborn ,  Michael VanAuker ,  Ryan Toomey ,  Elizabeth Hood

    发明人： Norma Alcantar ,  Kristina Dearborn ,  Michael VanAuker ,  Ryan Toomey ,  Elizabeth Hood

    IPC分类号： A61K9/50

    CPC分类号： A61K9/0014 ,  A61K9/0085 ,  A61K47/36

    摘要： Taught herein is a drug-delivery system that includes encapsulating a therapeutic drug in a nanoparticle vesicle that is then embedded into a hydrogel network. The system allows for enhanced, two-fold control over the release rate of the drug. This technology will be particularly advantageous in treating malignant cancer cells such as those found in the brain. The invention will allow for decreased side effects and increased survival time in patients. This invention opens the door to other technological applications that require controlled release of chemical substances.

    摘要翻译： 本文所述是药物递送系统，其包括将治疗药物包封在纳米颗粒囊泡中，然后将其嵌入水凝胶网络中。 该系统允许对药物的释放速率进行增强的双重控制。 该技术在治疗恶性癌细胞如脑中发现的这些细胞方面特别有利。 本发明将允许患者的副作用减少和生存时间增加。 本发明为需要化学物质控制释放的其他技术应用打开了大门。