公开(公告)号：US20180223297A1

公开(公告)日：2018-08-09

申请号：US15943106

申请日：2018-04-02

Applicant: Dow AgroSciences LLC ,  Sangamo Therapeutics, Inc.

Inventor： Noel Cogan ,  John Forster ,  Matthew Hayden ,  Tim Sawbridge ,  German Spangenberg ,  Steven R. Webb ,  Manju Gupta ,  W. Michael Ainley ,  Matthew J. Henry ,  Jeffrey C. Miller ,  Dmitry Y. Guschin

IPC: C12N15/82 ,  C07K14/47 ,  C12N9/22 ,  C12N15/90 ,  C12N15/66 ,  C12N15/10

Abstract: A method of gene editing or gene stacking within a FAD2 loci by cleaving, in a site directed manner, a location in a FAD2 gene in a cell, to generate a break in the FAD2 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.

公开(公告)号：US11198883B2

公开(公告)日：2021-12-14

申请号：US16674831

申请日：2019-11-05

Applicant: Dow AgroSciences LLC ,  Sangamo Therapeutics, Inc.

Inventor： W. Michael Ainley ,  Dmitry Y. Guschin ,  Matthew Hayden ,  Daniel Isenegger ,  John Mason ,  Jeffrey C. Miller ,  Joseph F. Petolino ,  Yidong Ran ,  Tim Sawbridge ,  German Spangenberg ,  Steven R. Webb

IPC: C12N15/82 ,  C12N15/90

Abstract: Disclosed herein are methods and compositions for parallel or sequential transgene stacking in plants to produce plants with selected phenotypes.

公开(公告)号：US10287595B2

公开(公告)日：2019-05-14

申请号：US15292774

申请日：2016-10-13

Applicant: Dow AgroSciences LLC ,  Sangamo Therapeutics, Inc.

Inventor： W. Michael Ainley ,  Steven R. Webb ,  Jayakumar P. Samuel ,  Dmitry Y. Guschin ,  Jeffrey C. Miller ,  Lei Zhang

IPC: C12N15/82 ,  C07K14/00 ,  C12N15/66 ,  C12N9/22 ,  C12N15/10 ,  C12N15/90 ,  C07K14/47

Abstract: Methods and compositions for gene disruption, gene editing or gene stacking within a FAD2 loci by cleaving, in a site directed manner, a location in a FAD2 gene in a soybean cell, to generate a break in the FAD2 gene and then optionally integrating into the break a nucleic acid molecule of interest is disclosed.

公开(公告)号：US10174331B2

公开(公告)日：2019-01-08

申请号：US13889162

申请日：2013-05-07

Applicant: Sangamo BioSciences, Inc. ,  Dow AgroSciences LLC

Inventor： Gregory J. Cost ,  Fyodor Urnov ,  W. Michael Ainley ,  Joseph F. Petolino ,  Jayakumar Pon Samuel ,  Steven R. Webb ,  Lakshmi Sastry-Dent

IPC: C12N15/82 ,  C12N15/87 ,  C12N15/85 ,  C12N15/10

Abstract: Disclosed herein are methods and compositions for homology-independent targeted insertion of donor molecules into the genome of a cell.

公开(公告)号：US09909131B2

公开(公告)日：2018-03-06

申请号：US14531748

申请日：2014-11-03

Applicant: Dow AgroSciences LLC

Inventor： Lakshmi Sastry-Dent ,  Zehui Cao ,  Shreedharan Sriram ,  Steven R. Webb ,  Debra L. Camper ,  W. Michael Ainley

IPC: C12N15/82 ,  A01H5/10

CPC classification number: C12N15/8213 ,  A01H5/10 ,  C12N15/8243 ,  C12N15/8273 ,  C12N15/8274 ,  C12N15/8286

Abstract: As disclosed herein, optimal native genomic loci of soybean plants have been identified that represent best sites for targeted insertion of exogenous sequences.

公开(公告)号：US09765404B2

公开(公告)日：2017-09-19

申请号：US14475968

申请日：2014-09-03

Applicant: Dow AgroSciences LLC

Inventor： Lakshmi Sastry-Dent ,  W. Michael Ainley ,  Jayakumar P. Samuel ,  Zehui Cao ,  Liu Y. Shen ,  Cristie M. Dewes

IPC: C12P19/34 ,  C12Q1/68

CPC classification number: C12Q1/6895

Abstract: The present disclosure provides methods for detecting and identifying plant events that contain donor sequences inserted precisely into targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method comprises the steps of amplifying a genomic DNA with a first round of PCR to produce a first amplicon from donor sequences inserted in the reverse orientation, amplifying the first amplicon with a second round of PCR and detecting the presence of the second amplicon, wherein the production of the first and second amplicon indicates the presence of the site specific integration event.

公开(公告)号：US10526610B2

公开(公告)日：2020-01-07

申请号：US15882609

申请日：2018-01-29

Applicant: Dow AgroSciences LLC ,  Sangamo Therapeutics, Inc.

Inventor： Noel Cogan ,  John Forster ,  Matthew Hayden ,  Tim Sawbridge ,  German Spangenberg ,  Steven R. Webb ,  Manju Gupta ,  W. Michael Ainley ,  Matthew J. Henry ,  Jeffrey C. Miller ,  Dmitry Y. Guschin

IPC: C12N15/79 ,  C12N15/82 ,  C12N9/22 ,  C12N15/63

Abstract: A method of gene editing or gene stacking within a FAD3 loci by cleaving, in a site directed manner, a location in a FAD3 gene in a cell, to generate a break in the FAD3 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.

公开(公告)号：US20190316141A1

公开(公告)日：2019-10-17

申请号：US16392217

申请日：2019-04-23

Applicant: Dow AgroSciences LLC ,  Sangamo Therapeutics, Inc.

Inventor： W. Michael Ainley ,  Steven R. Webb ,  Jayakumar P. Samuel ,  Dmitry Y. Guschin ,  Jeffrey C. Miller ,  Lei Zhang

IPC: C12N15/82 ,  C07K14/47 ,  C12N15/10 ,  C12N9/22 ,  C12N15/90 ,  C12N15/66

Abstract: Methods and compositions for gene disruption, gene editing or gene stacking within a FAD2 loci by cleaving, in a site directed manner, a location in a FAD2 gene in a soybean cell, to generate a break in the FAD2 gene and then optionally integrating into the break a nucleic acid molecule of interest is disclosed.

公开(公告)号：US20180163217A1

公开(公告)日：2018-06-14

申请号：US15882609

申请日：2018-01-29

Applicant: Dow AgroSciences LLC ,  Sangamo Therapeutics, Inc.

Inventor： Noel Cogan ,  John Forster ,  Matthew Hayden ,  Tim Sawbridge ,  German Spangenberg ,  Steven R. Webb ,  Manju Gupta ,  W. Michael Ainley ,  Matthew J. Henry ,  Jeffrey C. Miller ,  Dmitry Y. Guschin

IPC: C12N15/79 ,  C12N9/22 ,  C12N15/82 ,  C12N15/63

CPC classification number: C12N15/79 ,  C12N9/22 ,  C12N15/63 ,  C12N15/8241 ,  C12N15/8247

Abstract: A method of gene editing or gene stacking within a FAD3 loci by cleaving, in a site directed manner, a location in a FAD3 gene in a cell, to generate a break in the FAD3 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.

公开(公告)号：US20140304853A1

公开(公告)日：2014-10-09

申请号：US14243553

申请日：2014-04-02

Applicant: Sangamo BioSciences, Inc. ,  Dow AgroSciences LLC

Inventor： W. Michael Ainley ,  Dmitry Y. Guschin ,  Matthew Hayden ,  Daniel Isenegger ,  John Mason ,  Jeffrey C. Miller ,  Joseph F. Petolino ,  Yidong Ran ,  Tim Sawbridge ,  German Spangenberg ,  Steven R. Webb

IPC: C12N15/82

CPC classification number: C12N15/8213 ,  C12N15/8274 ,  C12N15/8278 ,  C12N15/90 ,  C12N2800/80

Abstract: Disclosed herein are methods and compositions for parallel or sequential transgene stacking in plants to produce plants with selected phenotypes.

Abstract translation: 本文公开了用于在植物中平行或顺序转基因堆叠以产生具有选定表型的植物的方法和组合物。