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公开(公告)号：US11667886B2
公开(公告)日：2023-06-06
申请号：US16621572
申请日：2018-06-04
Applicant: DSM IP Assets B.V.
Inventor： Ioannis Papapetridis , Jacobus Thomas Pronk
CPC classification number: C12N1/16 , C12N1/185 , C12N15/52 , C12P7/10 , C12P2203/00 , C12R2001/865 , C12Y101/01008 , C12Y202/01001 , C12Y202/01002 , C12Y207/01019 , C12Y401/01039 , C12Y501/03001 , C12Y503/01006
Abstract: The present invention describes a recombinant yeast cell functionally expressing one or more heterologous nucleic acid sequences encoding for ribulose-1,5-phosphate carboxylase/oxygenase (EC4.1.1.39; Rubisco), and optionally one or more molecular chaperones for Rubisco, and one or more phosphoribulokinase (EC2.7.1.19; PRK), wherein one or more genes of the non-oxidative branch of the pentose phosphate pathway are overexpressed and/or wherein said yeast cell comprises a deletion or disruption of a glycerol-3-phosphate dehydrogenase (GPD) gene.
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公开(公告)号：US10883110B2
公开(公告)日：2021-01-05
申请号：US16893065
申请日：2020-06-04
Applicant: DSM IP Assets B.V.
Inventor： Jacobus Thomas Pronk , Antonius Jeroen Adriaan Van Maris , Victor Gabriel Guadalupe Medina
Abstract: The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD+-dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10) activity. The invention further relates to the use of a cell according to the invention in the preparation of ethanol.
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公开(公告)号：US10533181B2
公开(公告)日：2020-01-14
申请号：US16409197
申请日：2019-05-10
Applicant: DSM IP Assets B.V.
Inventor： Jacobus Thomas Pronk , Antonius Jeroen Adriaan Van Maris , Victor Gabriel Guadalupe Medina
Abstract: The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD+-dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10) activity. The invention further relates to the use of a cell according to the invention in the preparation of ethanol.
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公开(公告)号：US11214810B2
公开(公告)日：2022-01-04
申请号：US16833342
申请日：2020-03-27
Applicant: DSM IP Assets B.V.
Inventor： Jacobus Thomas Pronk , Antonius Jeroen Adriaan Van Maris , Victor Gabriel Guadalupe Medina
Abstract: The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD+-dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10) activity. The invention further relates to the use of a cell according to the invention in the preparation of ethanol.
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Patent Agency Ranking
公开(公告)号：US11198847B2
公开(公告)日：2021-12-14
申请号：US16966741
申请日：2019-01-31
Applicant: DSM IP ASSETS B.V.
Inventor： Antonius Jeroen Adriaan Van Maris , Jacobus Thomas Pronk , Maarten Dirk Verhoeven , Ioannis Papapetridis
Abstract: The invention relates to a method for preparing a yeast which is capable of simultaneously fermenting a pentose and a hexose sugar, the method comprising providing a yeast which comprises: one or more heterologous genes encoding an enzyme of a pentose metabolic pathway, a disruption of a gene encoding a ribulose-phosphate 3-epimerase and a disruption of a gene encoding a glucose-6-phosphate isomerase, and one or more overexpressed endogenous genes encoding an enzyme of the pentose phosphate pathway; and subjecting the yeast to evolutionary engineering on a medium comprising a hexose sugar and at least one pentose sugar, selecting for a yeast with improved growth rate when grown on a media comprising a hexose and at least one pentose sugar, so as to obtain an evolved yeast.
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公开(公告)号：US11186850B2
公开(公告)日：2021-11-30
申请号：US16858163
申请日：2020-04-24
Applicant: DSM IP ASSETS B.V.
Inventor： Ioannis Papapetridis , Antonius Jeroen Adriaan Van Maris , Jacobus Thomas Pronk , Paulus Petrus De Waal , Niels Lauret , Maarten Dirk Verhoeven , Jozef Petrus Johannes Schmitz
Abstract: The present invention describes a recombinant yeast cell functionally expressing one or more heterologous nucleic acid sequences encoding for ribulose-1,5-phosphate carboxylase/oxygenase (EC4.1.1.39; Rubisco), and optionally one or more molecular chaperones for Rubisco, and one or more phosphoribulokinase (EC2.7.1.19; PRK), wherein the phosphoribulokinase is under control of a promoter (the “PRK promoter”) that enables higher expression under anaerobic conditions than under aerobic conditions.
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公开(公告)号：US20190264217A1
公开(公告)日：2019-08-29
申请号：US16409197
申请日：2019-05-10
Applicant: DSM IP Assets B.V.
Inventor： Jacobus Thomas Pronk , Antonius Jeroen Adriaan Van Maris , Victor Gabriel Guadalupe Medina
Abstract: The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD+-dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10) activity. The invention further relates to the use of a cell according to the invention in the preparation of ethanol.
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公开(公告)号：US10913928B2
公开(公告)日：2021-02-09
申请号：US16471367
申请日：2017-12-18
Applicant: DSM IP Assets B.V.
Abstract: This invention relates to a recombinant cell, preferably a recombinant yeast cell comprising: a) a gene coding for an enzyme having glycerol-3-phosphate dehydrogenase activity, wherein said enzyme has a cofactor dependency for at least NADP+ and/or for NADPH; b) a gene encoding an enzyme having at least NAD+ dependent acetylating acetaldehyde dehydrogenase activity (EC 1.2.1.10); and c) a mutation or disruption in at least one gene selected from the group of GPD1 and GPD2. Said cell is suitable for ethanol production, has a reduced glycerol production at high ethanol yield.
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公开(公告)号：US09359613B2
公开(公告)日：2016-06-07
申请号：US14189989
申请日：2014-02-25
Applicant: DSM IP Assets B.V.
Inventor： Jacobus Thomas Pronk , Antonius Jeroen Adriaan Van Maris , Victor Gabriel Guadalupe Medina
Abstract: The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD+-dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10) activity. The invention further relates to the use of a cell according to the invention in the preparation of ethanol.
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