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公开(公告)号:US20170159058A1
公开(公告)日:2017-06-08
申请号:US15436623
申请日:2017-02-17
IPC分类号: C12N15/52 , C12N9/04 , C12N9/88 , C12P7/16 , C12N9/12 , C12N9/10 , C12N15/70 , C12N9/02 , C12N9/90
CPC分类号: C12N15/52 , C12N9/0006 , C12N9/0008 , C12N9/0073 , C12N9/1022 , C12N9/1205 , C12N9/1217 , C12N9/88 , C12N9/90 , C12N15/70 , C12P7/16 , C12P7/40 , C12P7/649 , C12Y101/01244 , C12Y102/01012 , C12Y114/13025 , C12Y202/01001 , C12Y207/01011 , C12Y207/0104 , C12Y207/02003 , C12Y401/02013 , C12Y401/02043 , C12Y402/01011 , C12Y501/03001 , C12Y503/01001 , C12Y503/01027 , Y02E50/10 , Y02E50/13
摘要: The present disclosure relates, in some aspects, to cell-free methods and systems for large-scale conversion of methane to isobutanol, comprising combining, in a bioreactor at elevated pressure, methane, oxygen, and cell lysates containing methane monooxygenase, methanol dehydrogenase, and enzymes that catalyze the conversion of formaldehyde to isobutanol, to form a cell-free reaction mixture, and incubating under suitable conditions the cell-free reaction to convert methane to isobutanol.
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公开(公告)号:US09611487B2
公开(公告)日:2017-04-04
申请号:US14137524
申请日:2013-12-20
CPC分类号: C12N15/52 , C12N9/0006 , C12N9/0008 , C12N9/0073 , C12N9/1022 , C12N9/1205 , C12N9/1217 , C12N9/88 , C12N9/90 , C12N15/70 , C12P7/16 , C12P7/40 , C12P7/649 , C12Y101/01244 , C12Y102/01012 , C12Y114/13025 , C12Y202/01001 , C12Y207/01011 , C12Y207/0104 , C12Y207/02003 , C12Y401/02013 , C12Y401/02043 , C12Y402/01011 , C12Y501/03001 , C12Y503/01001 , C12Y503/01027 , Y02E50/10 , Y02E50/13
摘要: The present disclosure relates, in some aspects, to cell-free methods and systems for large-scale conversion of methane to isobutanol, comprising combining, in a bioreactor at elevated pressure, methane, oxygen, and cell lysates containing methane monooxygenase, methanol dehydrogenase, and enzymes that catalyze the conversion of formaldehyde to isobutanol, to form a cell-free reaction mixture, and incubating under suitable conditions the cell-free reaction to convert methane to isobutanol.
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公开(公告)号:US20150275235A1
公开(公告)日:2015-10-01
申请号:US14434116
申请日:2013-10-15
申请人: DSM IP ASSETS B.V.
发明人: Maria Bernedina Elizabeth Jonkers , Paul Klaassen , Aloysius Wilhelmus Rudolphus Hubertus Teunissen , Rene Marcel De Jong
CPC分类号: C12P7/06 , C12N1/16 , C12N1/22 , C12N9/1022 , C12N9/1205 , C12N9/88 , C12N9/90 , C12N9/92 , C12P7/10 , C12Y202/01001 , C12Y202/01002 , C12Y207/01017 , C12Y404/01005 , C12Y501/03001 , C12Y503/01006 , Y02E50/16 , Y02E50/17
摘要: The invention relates to a cell capable of converting one or more pentose sugar and one or more hexose sugar into fermentation product constitutively expressing one or more heterologous or homologous polypeptide having the amino acid sequence set out in SEQ ID NO: 20, or a variant polypeptide thereof having at least 45% identity to SEQ ID NO 20. In an embodiment the heterologous polypeptide has glyoxalase activity.
摘要翻译: 本发明涉及一种能够将一种或多种戊糖和一种或多种己糖转化成组成型表达一种或多种具有SEQ ID NO:20所示氨基酸序列的异源或同源多肽的发酵产物的细胞,或变体多肽 其与SEQ ID NO 20具有至少45%的同一性。在一个实施方案中,异源多肽具有乙二醛酶活性。
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公开(公告)号:US09862975B2
公开(公告)日:2018-01-09
申请号:US14858571
申请日:2015-09-18
申请人: CARGILL INCORPORATED
发明人: Holly J. Jessen , Jian Yi , Joshua Lundorff , Hans Liao , Ana Negrete-Raymond , Pirkko Suominen , Aristos Aristidou
IPC分类号: C12N1/00 , C12N1/19 , C12N1/22 , C12P7/06 , C12N9/12 , C12N9/90 , C12P7/10 , C12N15/81 , C07K14/39 , C12N9/04 , C12N9/10 , C12N9/92 , C12P7/54
CPC分类号: C12P7/10 , C07K14/39 , C12N9/0006 , C12N9/1022 , C12N9/1205 , C12N9/90 , C12N9/92 , C12N15/815 , C12P7/06 , C12P7/54 , C12Y101/01 , C12Y101/01009 , C12Y101/0101 , C12Y202/01001 , C12Y202/01002 , C12Y207/01016 , C12Y207/01017 , C12Y501/03001 , C12Y501/03004 , C12Y503/01003 , C12Y503/01005 , C12Y503/01006 , Y02E50/16 , Y02E50/17
摘要: The present application discloses the identification of the novel K. marxianus xylose transporter genes KHT105 and RAG4, as well as the identification of a novel set of I. orientalis pentose phosphate pathway genes The present application further discloses a series of genetically modified yeast cells comprising various combinations of arabinose fermentation pathways, xylose fermentation pathways, pentose phosphate pathways, and/or xylose transporter genes, and methods of culturing these cells to produce ethanol in fermentation media containing xylose.
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5.
公开(公告)号:US09181563B2
公开(公告)日:2015-11-10
申请号:US14111415
申请日:2012-04-11
申请人: Holly J. Jessen , Jian Yi , Joshua Lundorff , Hans Liao , Ana Negrete-Raymond , Pirkko Suominen , Aristos Aristidou
发明人: Holly J. Jessen , Jian Yi , Joshua Lundorff , Hans Liao , Ana Negrete-Raymond , Pirkko Suominen , Aristos Aristidou
IPC分类号: C12N1/00 , C12N1/19 , C12N1/22 , C12P7/06 , C07K14/39 , C12N9/04 , C12N9/10 , C12N9/12 , C12N9/90 , C12N9/92 , C12P7/10 , C12N15/81
CPC分类号: C12P7/10 , C07K14/39 , C12N9/0006 , C12N9/1022 , C12N9/1205 , C12N9/90 , C12N9/92 , C12N15/815 , C12P7/06 , C12P7/54 , C12Y101/01 , C12Y101/01009 , C12Y101/0101 , C12Y202/01001 , C12Y202/01002 , C12Y207/01016 , C12Y207/01017 , C12Y501/03001 , C12Y501/03004 , C12Y503/01003 , C12Y503/01005 , C12Y503/01006 , Y02E50/16 , Y02E50/17
摘要: The present application discloses the identification of the novel K. marxianus xylose transporter genes KHT105 and RAG4, as well as the identification of a novel set of I. orientalis pentose phosphate pathway genes The present application further discloses a series of genetically modified yeast cells comprising various combinations of arabinose fermentation pathways, xylose fermentation pathways, pentose phosphate pathways, and/or xylose transporter genes, and methods of culturing these cells to produce ethanol in fermentation media containing xylose.
摘要翻译: 本申请公开了新型马克斯黄连木糖转运蛋白基因KHT105和RAG4的鉴定,以及一种新型东方山羊戊糖磷酸途径基因的鉴定本申请还公开了一系列基因修饰的酵母细胞,其包含各种 阿拉伯糖发酵途径的组合,木糖发酵途径,戊糖磷酸途径和/或木糖转运蛋白基因,以及培养这些细胞以在含有木糖的发酵培养基中产生乙醇的方法。
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6.
公开(公告)号:US20140038253A1
公开(公告)日:2014-02-06
申请号:US14111415
申请日:2012-04-11
申请人: Holly J. Jessen , Jian Yi , Joshua Lundorff , Hans Liao , Ana Negrete-Raymond , Pirkko Suominen , Aristos Aristidou
发明人: Holly J. Jessen , Jian Yi , Joshua Lundorff , Hans Liao , Ana Negrete-Raymond , Pirkko Suominen , Aristos Aristidou
CPC分类号: C12P7/10 , C07K14/39 , C12N9/0006 , C12N9/1022 , C12N9/1205 , C12N9/90 , C12N9/92 , C12N15/815 , C12P7/06 , C12P7/54 , C12Y101/01 , C12Y101/01009 , C12Y101/0101 , C12Y202/01001 , C12Y202/01002 , C12Y207/01016 , C12Y207/01017 , C12Y501/03001 , C12Y501/03004 , C12Y503/01003 , C12Y503/01005 , C12Y503/01006 , Y02E50/16 , Y02E50/17
摘要: The present application discloses the identification of the novel K. marxianus xylose transporter genes KHT105 and RAG4, as well as the identification of a novel set of I. orientalis pentose phosphate pathway genes The present application further discloses a series of genetically modified yeast cells comprising various combinations of arabinose fermentation pathways, xylose fermentation pathways, pentose phosphate pathways, and/or xylose transporter genes, and methods of culturing these cells to produce ethanol in fermentation media containing xylose.
摘要翻译: 本申请公开了新型马克斯黄连木糖转运蛋白基因KHT105和RAG4的鉴定,以及一种新型东方山羊戊糖磷酸途径基因的鉴定本申请还公开了一系列基因修饰的酵母细胞,其包含各种 阿拉伯糖发酵途径的组合,木糖发酵途径,戊糖磷酸途径和/或木糖转运蛋白基因,以及培养这些细胞以在含有木糖的发酵培养基中产生乙醇的方法。
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公开(公告)号:US20180201958A1
公开(公告)日:2018-07-19
申请号:US15744049
申请日:2016-07-21
CPC分类号: C12P7/16 , C12P7/625 , C12Y101/01044 , C12Y202/01001 , C12Y202/01002 , C12Y203/01008 , C12Y301/01031 , C12Y401/02009 , C12Y401/02013 , C12Y401/02022 , C12Y501/03001 , C12Y503/01001 , C12Y503/01006 , C12Y503/01009 , Y02E50/10
摘要: Provided is an engineered pathway that can function in a cell-free system, cellular system or a combination thereof to convert a sugar to a chemical or biofuel.
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8.
公开(公告)号:US20160002676A1
公开(公告)日:2016-01-07
申请号:US14858571
申请日:2015-09-18
申请人: Cargill Incorporated
发明人: Holly J. JESSEN , Jian YI , Joshua LUNDORFF , Hans LIAO , Ana NEGRETE-RAYMOND , Pirkko SUOMINEN , Aristos ARISTIDOU
CPC分类号: C12P7/10 , C07K14/39 , C12N9/0006 , C12N9/1022 , C12N9/1205 , C12N9/90 , C12N9/92 , C12N15/815 , C12P7/06 , C12P7/54 , C12Y101/01 , C12Y101/01009 , C12Y101/0101 , C12Y202/01001 , C12Y202/01002 , C12Y207/01016 , C12Y207/01017 , C12Y501/03001 , C12Y501/03004 , C12Y503/01003 , C12Y503/01005 , C12Y503/01006 , Y02E50/16 , Y02E50/17
摘要: The present application discloses the identification of the novel K. marxianus xylose transporter genes KHT105 and RAG4, as well as the identification of a novel set of I. orientalis pentose phosphate pathway genes The present application further discloses a series of genetically modified yeast cells comprising various combinations of arabinose fermentation pathways, xylose fermentation pathways, pentose phosphate pathways, and/or xylose transporter genes, and methods of culturing these cells to produce ethanol in fermentation media containing xylose.
摘要翻译: 本申请公开了新型马克斯黄连木糖转运蛋白基因KHT105和RAG4的鉴定,以及一种新型东方山羊戊糖磷酸途径基因的鉴定本申请还公开了一系列基因修饰的酵母细胞,其包含各种 阿拉伯糖发酵途径的组合,木糖发酵途径,戊糖磷酸途径和/或木糖转运蛋白基因,以及培养这些细胞以在含有木糖的发酵培养基中产生乙醇的方法。
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9.
公开(公告)号:US08911983B2
公开(公告)日:2014-12-16
申请号:US13711646
申请日:2012-12-12
发明人: Perry G G Caimi , Min Qi , Luan Tao , Paul V Viitanen , Jianjun Yang
CPC分类号: C12N15/74 , C12N9/1258 , C12N9/90 , C12N9/92 , C12P7/065 , C12P7/10 , C12Y207/07008 , C12Y501/03001 , C12Y503/01005 , C12Y503/01006 , Y02E50/16 , Y02E50/17
摘要: The endogenous pnp gene encoding polynucleotide phosphorylase in the Zymomonas genome was identified as a target for modification to provide improved xylose utilizing cells for ethanol production. The cells are in addition genetically modified to have increased expression of ribose-5-phosphate isomerase (RPI) activity, as compared to cells without this genetic modification, and are not limited in xylose isomerase activity in the absence of the pnp modification.
摘要翻译: 在酵母单位基因组中编码多核苷酸磷酸化酶的内源性pnp基因被鉴定为用于修饰以提供用于乙醇生产的细胞的改良木糖的靶标。 与没有这种遗传修饰的细胞相比,细胞另外进行遗传修饰以具有增加的核糖-5-磷酸异构酶(RPI)活性的表达,并且在没有pnp修饰的情况下不限制于木糖异构酶活性。
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公开(公告)号:US09594869B2
公开(公告)日:2017-03-14
申请号:US14001299
申请日:2012-03-01
申请人: Desmond Siu Men Lun
发明人: Desmond Siu Men Lun
IPC分类号: C12N1/21 , C12P7/64 , C12P5/02 , G06F19/12 , C12N9/02 , C12N9/10 , C12N9/90 , C12N1/20 , C10G3/00
CPC分类号: G06F19/12 , C10G3/50 , C12N1/20 , C12N9/001 , C12N9/1022 , C12N9/90 , C12P7/6409 , C12Y103/99003 , C12Y202/01002 , C12Y501/03001 , Y02P20/52 , Y02P30/20
摘要: This invention concerns methods of identifying genetic alterations with which a microbe can be used to produce fatty acids at a large amount for making biofuels. Also disclosed are microbes with such genetic alterations and uses thereof.
摘要翻译: 本发明涉及鉴定遗传变异的方法,通过该遗传改变微生物可用于大量生产用于制备生物燃料的脂肪酸。 还公开了具有这种遗传改变及其用途的微生物。
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