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    Large comb type branched polynucleotides
    12.
    发明授权
    Large comb type branched polynucleotides 失效
    大梳型支链多核苷酸

    公开(公告)号：US5710264A

    公开(公告)日：1998-01-20

    申请号：US478085

    申请日：1995-06-07

    申请人： Michael S. Urdea ,  Thomas Horn ,  Chu-An Chang ,  Brian Warner ,  Timothy J. Fultz

    发明人： Michael S. Urdea ,  Thomas Horn ,  Chu-An Chang ,  Brian Warner ,  Timothy J. Fultz

    IPC分类号： C07H21/00 ,  C12Q1/68 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C07H21/00 ,  C12Q1/682

    摘要： Large comb-type branched polynucleotides useful as amplifier molecules in nucleic acid hybridization assays are provided, the polynucleotides comprising: a polynucleotide backbone having at least about 15 multifunctional nucleotides, each of which defines a sidechain site and a first single-stranded oligonucleotide unit that is capable of binding specifically to a first single-stranded polynucleotide sequence of interest; and pendant polynucleotide sidechains extending from said multifunctional nucleotides each comprising iterations of a second single-stranded oligonucleotide unit that is capable of binding specifically to a second single-stranded polynucleotide sequence of interest, the total number of iterations in all sidechains being at least 20. Methods of making these polynucleotides are also provided.

    摘要翻译： 提供了用作核酸杂交测定中的放大分子的大梳状支化多核苷酸，所述多核苷酸包含：多核苷酸主链，其具有至少约15个多功能核苷酸，每个核苷酸限定侧链位点和第一单链寡核苷酸单元， 能够特异性结合目标的第一单链多核苷酸序列; 和从所述多功能核苷酸延伸的侧挂多核苷酸侧链，其每个包括能够特异性结合目标的第二单链多核苷酸序列的第二单链寡核苷酸单元的迭代，所有侧链的总迭代次数至少为20。 还提供制备这些多核苷酸的方法。

    Kits for nucleic acid sequence amplification methods
    19.
    发明授权
    Kits for nucleic acid sequence amplification methods 失效
    用于核酸序列扩增方法的试剂盒

    公开(公告)号：US5888779A

    公开(公告)日：1999-03-30

    申请号：US461654

    申请日：1995-06-05

    申请人： Daniel Louis Kacian ,  Timothy J. Fultz

    发明人： Daniel Louis Kacian ,  Timothy J. Fultz

    IPC分类号： C12Q1/68 ,  C12Q1/70 ,  C12P19/34

    CPC分类号： C12Q1/6855 ,  Y10S435/975

    摘要： Kits for synthesizing multiple copies of a target nucleic acid sequence autocatalytically under conditions of substantially constant temperature, ionic strength, and pH are provided in which multiple RNA copies of the target sequence autocatalytically generate additional copies. These methods are useful for generating copies of a nucleic acid target sequence for purposes which include assays to quantitate specific nucleic acid sequences in clinical, environmental, forensic and similar samples, cloning and generating probes.

    摘要翻译： 提供了在基本恒定温度，离子强度和pH条件下自动催化靶核酸序列的多个拷贝的试剂盒，其中靶序列的多个RNA拷贝自动催化产生额外的拷贝。 这些方法可用于产生核酸靶序列的拷贝，目的包括用于定量临床，环境，法医和相似样品中的特异性核酸序列的测定，克隆和产生探针。