公开(公告)号：US06559441B2

公开(公告)日：2003-05-06

申请号：US10074210

申请日：2002-02-12

Applicant: David E. Clemmer

Inventor： David E. Clemmer

IPC: H01J4940

CPC classification number: G01N27/622 ,  H01J49/004 ,  H01J49/38 ,  H01J49/40

Abstract: An ion separation instrument includes an ion source coupled to at least a first ion mobility spectrometer having an ion outlet coupled to a mass spectrometer. In one embodiment, the ion source includes a molecule separation instrument operable to separate ions in time according to a molecular characteristic such as ion retention time. The resultant instrument is thus operable to provide molecular information separated in time as functions of retention time, ion mobility and ion mass/charge. In another embodiment, the ion separation instrument includes first and second ion mobility instruments disposed in a cascade arrangement between the ion source and mass spectrometer, wherein the two ion mobility instruments are operable to separate ions in time each according to different ion mobility functions. For example, the two ion mobility instruments may have different flight tube lengths, operate at different temperatures, operate in the presence of different electric fields and/or operate in the presence of different gases. The resultant instrument is thus operable to provide molecular information separated in time according to at least two different functions of ion mobility as well as ion mass/charge.

公开(公告)号：US08513591B2

公开(公告)日：2013-08-20

申请号：US12952109

申请日：2010-11-22

Applicant: David E. Clemmer ,  Ruwan T. Kurulugama ,  Fabiane M. Nachtigall ,  Zachary Henson ,  Samuel I. Merenbloom ,  Stephen J. Valentine

Inventor： David E. Clemmer ,  Ruwan T. Kurulugama ,  Fabiane M. Nachtigall ,  Zachary Henson ,  Samuel I. Merenbloom ,  Stephen J. Valentine

IPC: H01J49/42

CPC classification number: G01N27/622

Abstract: An ion mobility spectrometer instrument has a drift tube that is partitioned into a plurality of cascaded drift tube segments. A number of electric field activation sources may each be coupled to one or more of the plurality of drift tube segments. A control circuit is configured to control operation of the number of electric field activation sources in a manner that sequentially applies electric fields to the drift tube segments to allow only ions having a predefined ion mobility or range of ion mobilities to travel through the drift tube. The drift tube segments may define a linear drift tube or a closed drift tube with a continuous ion travel path. Techniques are disclosed for operating the ion mobility spectrometer to produce highly resolved ion mobility spectra.

Abstract translation: 离子迁移谱仪具有漂移管，其被分隔成多个级联漂移管段。 多个电场激活源可以各自耦合到多个漂移管段中的一个或多个。 控制电路被配置为以顺序地向漂移管段施加电场以允许具有预定离子迁移率或离子迁移率范围的离子行进穿过漂移管的方式来控制电场激活源的数量的操作。 漂移管段可以限定具有连续离子行进路径的线性漂移管或闭合漂移管。 公开了用于操作离子迁移谱仪以产生高度分辨的离子迁移率光谱的技术。

公开(公告)号：US20110198493A1

公开(公告)日：2011-08-18

申请号：US13038544

申请日：2011-03-02

Applicant: David E. Clemmer ,  Stormy L. Koeniger ,  Stephen J. Valentine

Inventor： David E. Clemmer ,  Stormy L. Koeniger ,  Stephen J. Valentine

IPC: H01J49/40

CPC classification number: G01N27/622

Abstract: An ion mobility spectrometer comprises a drift tube defining a drift tube inlet configured to receive ions and a drift tube outlet. The drift tube is configured to separate ions in time as a function of ion mobility. The drift tube defines a first ion activation region between the drift tube inlet and the drift tube outlet. The first ion activation region is configured to selectively induce structural changes in at least some of the ions.

Abstract translation: 离子迁移谱仪包括漂移管，其限定配置成接收离子的漂移管入口和漂移管出口。 漂移管被配置为随着离子迁移率的时间分离离子。 漂移管限定漂移管入口和漂移管出口之间的第一离子激活区。 第一离子激活区被配置为选择性地诱导至少一些离子的结构变化。

公开(公告)号：US06498342B1

公开(公告)日：2002-12-24

申请号：US09615102

申请日：2000-07-13

Applicant: David E. Clemmer

Inventor： David E. Clemmer

IPC: H01J4940

CPC classification number: G01N27/622 ,  H01J49/004 ,  H01J49/38 ,  H01J49/40

Abstract: An ion separation instrument includes an ion source coupled to at least a first ion mobility spectrometer having an ion outlet coupled to a mass spectrometer. In one embodiment, the ion source includes a molecule separation instrument operable to separate ions in time according to a molecular characteristic such as ion retention time. The resultant instrument is thus operable to provide molecular information separated in time as functions of retention time, ion mobility and ion mass/charge. In another embodiment, the ion separation instrument includes first and second ion mobility instruments disposed in a cascade arrangement between the ion source and mass spectrometer, wherein the two ion mobility instruments are operable to separate ions in time each according to different ion mobility functions. For example, the two ion mobility instruments may have different flight tube lengths, operate at different temperatures, operate in the presence of different electric fields and/or operate in the presence of different gases. The resultant instrument is thus operable to provide molecular information separated in time according to at least two different functions of ion mobility as well as ion mass/charge.

Abstract translation: 离子分离仪器包括耦合到具有耦合到质谱仪的离子出口的至少第一离子迁移谱仪的离子源。 在一个实施方案中，离子源包括可以根据诸如离子保留时间的分子特征在时间上分离离子的分子分离仪器。 所得到的仪器因此可操作以提供在时间上分离的分子信息作为保留时间，离子迁移率和离子质量/电荷的函数。 在另一个实施方案中，离子分离仪器包括以离子源和质谱仪之间的级联布置设置的第一和第二离子迁移性仪器，其中两种离子迁移仪器可操作以按照不同的离子迁移功能在时间上分离离子。 例如，两个离子迁移仪器可具有不同的飞行管长度，在不同温度下操作，在存在不同电场的情况下操作和/或在存在不同气体的情况下操作。 因此，所得仪器可操作地提供根据离子迁移率以及离子质量/电荷的至少两个不同功能在时间上分离的分子信息。

公开(公告)号：US06323482B1

公开(公告)日：2001-11-27

申请号：US09313492

申请日：1999-05-17

Applicant: David E. Clemmer ,  James P. Reilly

Inventor： David E. Clemmer ,  James P. Reilly

IPC: H01J4940

CPC classification number: G01N27/622 ,  H01J49/004 ,  H01J49/40 ,  H01J49/42

Abstract: An ion mobility and mass spectrometer instrument includes an ion source region coupled to an ion mobility spectrometer having an ion outlet coupled to a quadrupole mass filter. An output of the filter is coupled to a collision cell which has an ion outlet coupled to an ion acceleration region of a mass spectrometer such as a time of flight mass spectrometer. The instrument is particularly well suited for sequencing analysis wherein a sample is ionized and a resulting three-dimensional ion spectrum (ion intensity vs. ion mobility and ion mass) is observed. If the spectrum reveals that no ions overlap in mobility values, the collision cell is filled with a suitable buffer gas and the instrument is reactivated whereby a complete three-dimensional spectrum of parent and daughter ions results. If, however, the original spectrum reveals that two or more ions overlap in ion mobility values, the collision cell is filled with a buffer gas and the quadrupole mass filter is controlled to selectively filter out all but one of the ions having overlapping mobility values. The instrument is reactivated, and the quadrupole mass filter is selectively controlled, as many times as mobility overlap occurs to thereby provide complete three-dimensional spectra of parent and daughter ions resulting from fragmentation. Various configurations of mass filter, ion trap and collision cell positioning, relative to the ion mobility and mass spectrometer instruments, are contemplated.

Abstract translation: 离子迁移率和质谱仪器包括耦合到具有耦合到四极质量过滤器的离子出口的离子迁移谱仪的离子源区域。 滤波器的输出耦合到具有耦合到诸如飞行时间质谱仪的质谱仪的离子加速度区域的离子出口的碰撞池。 该仪器特别适用于样品离子化的测序分析，并且观察到所得的三维离子光谱（离子强度对离子迁移率和离子质量）。 如果光谱显示没有离子在迁移率值上重叠，则碰撞池填充有合适的缓冲气体，并且仪器被再活化，由此产生完整的母体和子离子的三维光谱。 然而，如果原始光谱显示两个或更多个离子在离子迁移率值中重叠，则碰撞单元填充有缓冲气体，并且控制四极质量过滤器以选择性地过滤掉具有重叠迁移率值的所有离子之外的所有离子。 仪器被重新激活，并且四极质量过滤器被选择性地控制，发生迁移重叠的次数多，从而提供由碎裂产生的母体和子体离子的完整的三维光谱。 考虑到相对于离子迁移率和质谱仪器的质量过滤器，离子阱和碰撞池定位的各种配置。