公开(公告)号：US5914451A

公开(公告)日：1999-06-22

申请号：US56073

申请日：1998-04-06

Applicant: Brian Martinell ,  Lori A. Julson ,  Maud A. W. Hinchee ,  Dannette Connor-Ward ,  Dennis McCabe ,  Carl Emler

Inventor： Brian Martinell ,  Lori A. Julson ,  Maud A. W. Hinchee ,  Dannette Connor-Ward ,  Dennis McCabe ,  Carl Emler

IPC: C12N15/09 ,  A01H1/04 ,  A01H4/00 ,  C12N5/10 ,  C12N15/82 ,  A01H5/00 ,  C12N5/04

CPC classification number: A01H4/001 ,  A01H1/04 ,  C12N15/8201 ,  C12N15/8207 ,  C12N15/8209 ,  C12N15/8275

Abstract: A transformation protocol is described for the particle mediated transformation of soybean. This protocol is based on gene delivery into the growing meristem of a soybean embryo. Prior transformation protocols based on meristematic gene delivery in soybean did not depend on selection due to difficulties in using selection agents in differentiated tissue in soybean. It has been found that a post bombardment culture with glyphosate selection can dramatically increase the efficiency of such a transformation protocol.

Abstract translation: 描述了用于大豆的颗粒介导的转化的转化方案。 该方案基于将基因递送到大豆胚胎生长的分生组织中。 在大豆中基于分生组织基因递送的先前转化方案不依赖于选择，因为在大豆分化组织中使用选择剂的困难。 已经发现，具有草甘膦选择的后轰击培养可以显着提高这种转化方案的效率。

公开(公告)号：US4530834A

公开(公告)日：1985-07-23

申请号：US419637

申请日：1982-09-17

Applicant: Dennis McCabe ,  Richard S. Soper

Inventor： Dennis McCabe ,  Richard S. Soper

IPC: C12N1/14 ,  A01N63/04 ,  A01N65/00 ,  C12N1/04 ,  C12N1/20 ,  C12R1/645 ,  A01N63/00 ,  C12N1/00 ,  C12N3/00

CPC classification number: C12N1/04 ,  A01N63/04 ,  Y10S435/911

Abstract: Entomopathogenic fungi useful in the control of insects is prepared by culturing mycelia in a suitable medium, harvesting the growing mycelia, treating the mycelia with a protective agent, drying the treated mycelia and grinding and storing the dried product.

Abstract translation: 用于控制昆虫的昆虫病原真菌通过在合适的培养基中培养菌丝体，收获生长的菌丝体，用保护剂处理菌丝体，干燥经处理的菌丝体并研磨和储存干燥的产物来制备昆虫病原真菌。