公开(公告)号：US20250027144A1

公开(公告)日：2025-01-23

申请号：US18415517

申请日：2024-01-17

Applicant: Element Biosciences, Inc.

Inventor： Sinan ARSLAN ,  Molly HE ,  Michael PREVITE

IPC: C12Q1/6869 ,  G01N15/1434 ,  G01N21/64

Abstract: Nucleic acid hybridization buffer formulations and uses thereof are described that yield improvements in hybridization specificity, rate, and efficiency. The buffer formulation composition includes a target nucleic acid; at least one polar, aprotic, organic solvent, and a pH buffer system, wherein the target nucleic acid is attached to the surface via hybridization to a surface bound nucleic acid tethered to the surface, and wherein the hybridization of the target nucleic acid and surface bound nucleic acid has a high stringency and annealing rate.

公开(公告)号：US20240382956A1

公开(公告)日：2024-11-21

申请号：US18680203

申请日：2024-05-31

Applicant: Element Biosciences, Inc.

Inventor： Minghao GUO ,  Leon Zilun ZHANG ,  Chunhong ZHOU ,  Matthew KELLINGER ,  Michael PREVITE

IPC: B01L3/00

Abstract: Flow cell devices, cartridges, and systems are described that provide reduced manufacturing complexity, lowered consumable costs, and flexible system throughput for nucleic acid sequencing and other chemical or biological analysis applications. The flow cell device can include a capillary flow cell device or a microfluidic flow cell device.

公开(公告)号：US20230323450A1

公开(公告)日：2023-10-12

申请号：US18181476

申请日：2023-03-09

Applicant: Element Biosciences, Inc.

Inventor： Sinan Arslan ,  Molly Min HE ,  Matthew KELLINGER ,  Jake LEVIEUX ,  Michael PREVITE ,  Junhua ZHAO ,  Su ZHANG ,  Tyler LOPEZ

IPC: C12Q1/6874 ,  G01N33/58 ,  G01N33/53

CPC classification number: C12Q1/6874 ,  G01N33/582 ,  G01N33/5308

Abstract: Multivalent binding compositions including a particle-nucleotide conjugate having a plurality of copies of a nucleotide attached to the particle are described. The multivalent binding compositions allow one to localize detectable signals to active regions of biochemical interaction, e.g., sites of protein-protein interaction, protein-nucleic acid interaction, nucleic acid hybridization, or enzymatic reaction, and can be used to identify sites of base incorporation in elongating nucleic acid chains during polymerase reactions and to provide improved base discrimination for sequencing and array based applications.

公开(公告)号：US20230235392A1

公开(公告)日：2023-07-27

申请号：US17999023

申请日：2021-05-19

Applicant: Element Biosciences, Inc.

Inventor： Sinan ARSLAN ,  Junhua ZHAO ,  Molly HE ,  William LIGHT ,  Matthew KELLINGER ,  Michael PREVITE

IPC: C12Q1/6874 ,  C12Q1/6855 ,  C12Q1/6876 ,  C12Q1/6806

CPC classification number: C12Q1/6874 ,  C12Q1/6855 ,  C12Q1/6876 ,  C12Q1/6806 ,  C12Q2600/16

Abstract: Provided herein are methods for generating circular nucleic acid molecules and circular nucleic acid libraries. The methods can be used to generate clonal populations of target nucleic acid molecules for downstream applications such as sequencing. Nucleic acid sequence methods, systems and kits are also provided for sequencing circular nucleic acid molecules.

公开(公告)号：US20230167434A1

公开(公告)日：2023-06-01

申请号：US18153268

申请日：2023-01-11

Applicant: Element Biosciences, Inc.

Inventor： Matthew KELLINGER ,  Sinan ARSLAN ,  Michael PREVITE ,  Junhua ZHAO

IPC: C12N15/10 ,  C12N9/12 ,  C12Q1/6869

CPC classification number: C12N15/1068 ,  C12N9/12 ,  C12N9/1241 ,  C12Q1/6869

Abstract: Provided herein are methods for generating circular nucleic acid molecules and circular nucleic acid libraries. The methods can be used to generate clonal populations of target nucleic acid molecules for downstream applications such as sequencing.

公开(公告)号：US20220251644A1

公开(公告)日：2022-08-11

申请号：US17723280

申请日：2022-04-18

Applicant: Element Biosciences, Inc.

Inventor： Steve Xiangling CHEN ,  Minghao GUO ,  Michael PREVITE ,  Chunhong ZHOU ,  Derek FULLER

IPC: C12Q1/6869 ,  G01N21/64 ,  G01N15/14 ,  C12N15/10 ,  C12Q1/6806

Abstract: Imaging systems and methods comprising imaging a first interior surface and a second interior surface of a flow cell are described. In some embodiments, the imaging systems may comprise: a) an objective lens; b) at least one image sensor; and c) at least one tube lens disposed in an optical path between the objective lens and the at least one image sensor; wherein the at least one tube lens is configured to correct imaging performance such that images of the first interior surface of the flow cell and the second interior surface of the flow cell have substantially the same optical resolution.

公开(公告)号：US20220186310A1

公开(公告)日：2022-06-16

申请号：US17511046

申请日：2021-10-26

Applicant: Element Biosciences, Inc.

Inventor： Sinan Arslan ,  Molly Min HE ,  Matthew KELLINGER ,  Jake LEVIEUX ,  Michael PREVITE ,  Junhua ZHAO ,  Su ZHANG ,  Tyler LOPEZ

IPC: C12Q1/6874 ,  G01N33/53 ,  G01N33/58

Abstract: Multivalent binding compositions including a particle-nucleotide conjugate having a plurality of copies of a nucleotide attached to the particle are described. The multivalent binding compositions allow one to localize detectable signals to active regions of biochemical interaction, e.g., sites of protein-protein interaction, protein-nucleic acid interaction, nucleic acid hybridization, or enzymatic reaction, and can be used to identify sites of base incorporation in elongating nucleic acid chains during polymerase reactions and to provide improved base discrimination for sequencing and array based applications.

公开(公告)号：US20220136047A1

公开(公告)日：2022-05-05

申请号：US17574416

申请日：2022-01-12

Applicant: Element Biosciences, Inc.

Inventor： Steve Xiangling CHEN ,  Minghao GUO ,  Michael PREVITE ,  Chunhong ZHOU ,  Derek FULLER

IPC: C12Q1/6869 ,  G01N21/64 ,  G01N15/14 ,  C12N15/10 ,  C12Q1/6806

Abstract: Fluorescence imaging system designs are described that provide larger fields-of-view, increased spatial resolution, improved modulation transfer and image quality, higher spatial sampling frequency, faster transitions between image capture when repositioning the sample plane to capture a series of images (e.g., of different fields-of-view), and improved imaging system duty cycle, and thus enable higher throughput image acquisition and analysis for genomics and other imaging applications.

公开(公告)号：US20210332430A1

公开(公告)日：2021-10-28

申请号：US17373655

申请日：2021-07-12

Applicant: Element Biosciences, Inc.

Inventor： Sinan ARSLAN ,  Molly HE ,  Michael PREVITE ,  Steve Xiangling CHEN ,  Minghao GUO ,  Chunhong ZHOU ,  Derek FULLER

IPC: C12Q1/6869 ,  G01N21/64 ,  G01N15/14

Abstract: Fluorescence imaging system designs are described that provide larger fields-of-view, increased spatial resolution, improved modulation transfer and image quality, higher spatial sampling frequency, faster transitions between image capture when repositioning the sample plane to capture a series of images (e.g., of different fields-of-view), and improved imaging system duty cycle, and thus enable higher throughput image acquisition and analysis for genomics and other imaging applications.

公开(公告)号：US20210223161A1

公开(公告)日：2021-07-22

申请号：US17016350

申请日：2020-09-09

Applicant: Element Biosciences, Inc.

Inventor： Steve Xiangling CHEN ,  Minghao GUO ,  Michael PREVITE ,  Chunhong ZHOU ,  Derek FULLER

IPC: G01N15/14

Abstract: Imaging systems and methods comprising imaging a first interior surface and a second interior surface of a flow cell are described. In some embodiments, the imaging systems may comprise: a) an objective lens; b) at least one image sensor; and c) at least one tube lens disposed in an optical path between the objective lens and the at least one image sensor; wherein the at least one tube lens is configured to correct imaging performance such that images of the first interior surface of the flow cell and the second interior surface of the flow cell have substantially the same optical resolution.