公开(公告)号：US06346414B1

公开(公告)日：2002-02-12

申请号：US08532657

申请日：1995-10-16

申请人： Eric Jacobs

发明人： Eric Jacobs

IPC分类号： C12N1579

CPC分类号： C12N15/90 ,  C12N9/22

摘要： A transposition assembly for the transfer of a DNA fragment of interest into the ribosomal nuclear DNA of an eukaryotic cell. An insertion means, an eukaryotic cell and a pharmaceutical composition comprising said transposition assembly, as well as a method for the in vitro transfer of said DNA fragment, are also disclosed.

摘要翻译： 用于将感兴趣的DNA片段转移到真核细胞的核糖体核DNA中的转座组件。 还公开了插入装置，真核细胞和包含所述转座组件的药物组合物，以及用于体外转移所述DNA片段的方法。

公开(公告)号：US06103519A

公开(公告)日：2000-08-15

申请号：US443054

申请日：1995-05-17

申请人： Martin Comberbach ,  Nigel Harford ,  Teresa Cabezon ,  Apolonia Rutgers ,  Pierre Voet ,  Eric Jacobs ,  Cornelis P. Hollenberg ,  Zbigniew A. Janowicz ,  Armin J. Merckelbach

发明人： Martin Comberbach ,  Nigel Harford ,  Teresa Cabezon ,  Apolonia Rutgers ,  Pierre Voet ,  Eric Jacobs ,  Cornelis P. Hollenberg ,  Zbigniew A. Janowicz ,  Armin J. Merckelbach

IPC分类号： A61K39/00 ,  A61K39/29 ,  A61P31/12 ,  C07K14/02 ,  C12N1/19 ,  C12N15/63

CPC分类号： C07K14/005 ,  A61K39/00 ,  C12N2730/10122

摘要： A composite particle comprising at least two polypeptides corresponding to all or part of a protein having the biological activity of one of the hepatitis B surface antigens, wherein the particle presents at least two antigenic determinants provided by the S-protein, preS2-protein or preS1-protein, said particle optionally further comprising host specific lipids. The invention also provides a modified hepatitis B surface antigen L protein which may be used alone or incorporated into such composite particles.A particular embodiment of the modified L protein (L*) comprises residues 12-52 followed by residues 133-145 followed by residues 175-400 of the L protein and a particular embodiment of a composite particle has the form (L*, S) where S is the S-protein of HBsAg.Improved hepatitis B vaccines may be prepared following expression of the above immunogens, especially in yeasts such as S. cerevisiae and Hansenula polymorpha.

摘要翻译： 包含至少两个对应于具有乙型肝炎表面抗原之一的生物活性的蛋白质全部或部分的多肽的复合颗粒，其中所述颗粒呈现由S-蛋白质，preS2-蛋白质或preS1提供的至少两种抗原决定簇 所述颗粒任选地还包含宿主特异性脂质。 本发明还提供了可以单独使用或并入这种复合颗粒中的改良的乙型肝炎表面抗原L蛋白。 修饰的L蛋白（L *）的一个具体实施方案包括残基12-52，其后是残基133-145，随后是L蛋白的残基175-400，复合颗粒的具体实施方案具有形式（L *，S） 其中S是HBsAg的S-蛋白。 改进的乙型肝炎疫苗可以在上述免疫原的表达后制备，特别是在酵母如酿酒酵母和多形汉逊酵母中。

公开(公告)号：US6028049A

公开(公告)日：2000-02-22

申请号：US448194

申请日：1995-05-23

申请人： Eric Jacobs ,  Michele Legrain ,  Veronique Mazarin ,  Bernadette Bouchon-Theisen ,  Anthony B. Schryvers ,  Marie-Aline Bloch

发明人： Eric Jacobs ,  Michele Legrain ,  Veronique Mazarin ,  Bernadette Bouchon-Theisen ,  Anthony B. Schryvers ,  Marie-Aline Bloch

IPC分类号： A61K39/095 ,  A61P31/04 ,  C07K14/22 ,  C07K14/705 ,  C07K16/12 ,  C12N1/21 ,  C12N15/09 ,  C12N15/31 ,  C12P21/02 ,  C12P21/08 ,  C12R1/19 ,  A61K38/00

CPC分类号： C07K14/22 ,  C07K14/70582 ,  C07K16/1217

摘要： The subject of the present invention is a DNA fragment which encodes a protein capable of being recognised by an antiserum against the transferrin receptor of the strain IM2394 or IM2169 of N. meningitidis as well as a process for producing the said protein by a recombinant route. By way of example, such a DNA fragment encodes the tbp1 subunit of the strain IM2394 or IM2169 or the tbp2 subunit of the strain IM2394 or IM2169.

摘要翻译： 本发明的主题是编码能够被来自脑膜炎奈瑟氏球菌的IM2394或IM2169的转铁蛋白受体的抗血清识别的蛋白质的DNA片段，以及通过重组途径制备所述蛋白质的方法。 举例来说，这样的DNA片段编码菌株IM2394或IM2169或菌株IM2394或IM2169的tbp2亚基的tbp1亚基。

公开(公告)号：US20100160227A1

公开(公告)日：2010-06-24

申请号：US12600460

申请日：2008-01-29

申请人： Nathalie Silvestre ,  Eric Jacobs

发明人： Nathalie Silvestre ,  Eric Jacobs

IPC分类号： A61K38/16 ,  C07K14/00 ,  C07H21/00 ,  C12N15/74 ,  C12N7/01 ,  C12N5/10 ,  A61K31/7088 ,  C12P21/00 ,  A61P43/00

CPC分类号： C12N15/85 ,  C07K14/005 ,  C07K2319/02 ,  C07K2319/03 ,  C12N2760/20122 ,  C12N2840/20

摘要： The present invention provides novel peptides of specified sequence and their use as a signal peptide or a membrane-anchoring peptide. It also relates to chimeric polypeptide comprising one or more such peptides and a polypeptide of interest as well as nucleic acid molecules, vectors, infections vital particles and host cells encoding such peptides and chimeric polypeptides. The present invention also relates to a pharmaceutical composition comprising such elements and a pharmaceutically acceptable vehicle. The present invention also provides a method for recumbently producing a polypeptide using such peptides, especially for directing expression of a polypeptide of interest extracellularly or anchored at the surface of the plasma membrane.

摘要翻译： 本发明提供具有特定序列的新型肽及其作为信号肽或膜 - 锚定肽的用途。 它还涉及包含一种或多种此类肽和感兴趣多肽以及核酸分子，载体，感染重要颗粒和编码这些肽和嵌合多肽的宿主细胞的嵌合多肽。 本发明还涉及包含这些元件和药学上可接受的载体的药物组合物。 本发明还提供了一种使用这些肽沉降产生多肽的方法，特别是用于引导细胞外或锚定在质膜表面的感兴趣多肽的表达。

公开(公告)号：US20070092469A1

公开(公告)日：2007-04-26

申请号：US11527334

申请日：2006-09-27

申请人： Eric Jacobs

发明人： Eric Jacobs

IPC分类号： A61K31/7024 ,  A61K31/7008 ,  A61K8/73

CPC分类号： A61Q19/08 ,  A61K8/60 ,  A61K8/676 ,  A61K31/7008 ,  A61K31/7024

摘要： A topical skin rejuvenation preparation to relieve wrinkles, increase the skin's natural production of hyaluronic acid, reverse the lack of suppleness, hydrate from within, erase spider veins, reduce varicose veins, lighten aging dark blotches (“liver spots”/Lentigos, Senile Lentigines), decrease acne, and reduce under eye puffiness includes Glucosamine (2-amino-2-deoxy-alpha-D-glucose), a hexosamine (6 carbon amino sugar), including its derivative and precursor compounds: Glucosamine Sulfate, Glucosamine Hydrochloride, Glucose-6-Phosphate, Acetyl Glucosamine, Fructose-6-phosphate, Glucosamine-6-Phosphate, to increase production of Hyaluronic acid and collagen from Glucosamine Sulfate, its precursors and derivatives and to increase skin muscle tone by Dimethylaminoethanol (DMAE) while over coming deficiency it creates in each cell's production of PhosphatidylCholine, whose deficiency damages cell membranes, as well as mitochondrial and lysosome membranes.

摘要翻译： 一种局部皮肤复原制剂，可缓解皱纹，增加皮肤天然产生的透明质酸，扭转肌肤柔软，水分从内，消除蛛网膜静脉曲张，减轻静脉曲张，减轻老化黑斑（“肝斑”/ Lentigos，Senile Lentigines ），减少痤疮，减少眼睛浮肿，包括葡糖胺（2-氨基-2-脱氧-α-D-葡萄糖），己糖胺（6碳氨基糖），包括其衍生物和前体化合物：硫酸葡萄糖胺，盐酸葡萄糖胺， 葡萄糖-6-磷酸，乙酰葡糖胺，果糖-6-磷酸，葡糖胺-6-磷酸，以增加硫酸葡萄糖胺，其前体和衍生物的透明质酸和胶原蛋白的产生，并通过二甲基氨基乙醇（DMAE）增加皮肤肌张力，同时超过 在每个细胞的磷脂酰胆碱生产中产生的缺陷，其缺陷损伤细胞膜，以及线粒体和溶酶体膜。