公开(公告)号：US20200308612A1

公开(公告)日：2020-10-01

申请号：US16308890

申请日：2017-07-19

Applicant: Firmenich SA

Inventor： Michel SCHALK ,  Letizia ROCCI

IPC: C12P7/62 ,  C12P5/00 ,  C12N9/88 ,  C12N9/02 ,  C12N15/70 ,  C12P7/22 ,  C12P7/26

Abstract: Provided is a method of producing one or more sesquiterpene compounds comprising: contacting an acyclic FPP precursor with a polypeptide having terpene synthase activity, wherein the polypeptide comprises an amino acid sequence that has at least 55% sequence identity to SEQ ID NO: 1, to produce one or more terpenes selected from the group consisting of isovalencene, spirovetiva-1(10),7(11)-diene and valencene or derivatives thereof, or mixture of sesquiterpenes comprising one or more of isovalencene, spirovetiva-1(10),7(11)-diene and/or valencene; and optionally isolating the one or more terpenes or the mixture. Also described is a nucleic acid derived from Vetiveria zizanoides encoding a polypeptide having sesquiterpene synthase activity, a polypeptide that can be used to produce one or more sesquiterpenes or a mixture of sesquiterpenes comprising one or more of isovalencene, spirovetiva-1(10),7(11)-diene and/or valencene, and a non-human organism or cell comprising the nucleic acid or comprising an expression vector comprising the nucleic acid.

公开(公告)号：US20180208946A1

公开(公告)日：2018-07-26

申请号：US15877183

申请日：2018-01-22

Applicant: FIRMENICH SA

Inventor： Laurent DAVIET ,  Letizia ROCCI ,  Michel SCHALK

IPC: C12P5/00 ,  C12N9/02 ,  C12P7/02

CPC classification number: C12P5/007 ,  C12N9/0071 ,  C12N9/0079 ,  C12P7/02 ,  C12Y114/13 ,  C12Y114/14001

Abstract: This invention relates generally to methods and compositions for producing a sesquiterpene alcohol comprising contacting a sesquiterpene with a P450 polypeptide with monooxygenase activity.

公开(公告)号：US20160177288A1

公开(公告)日：2016-06-23

申请号：US15060060

申请日：2016-03-03

Applicant: FIRMENICH SA

Inventor： Michel SCHALK

IPC: C12N9/88

CPC classification number: C12N9/88 ,  C12P5/007 ,  C12P15/00 ,  C12Y402/03082

Abstract: The present invention provides a method of producing α-santalene by contacting at least one polypeptide with farnesyl phyrophosphate (fpp). In particular, the method may be carried out in vitro or in vivo to produce α-santalene, a very useful compound in the fields of perfumery and flavoring. The present invention also provides the amino acid sequence of a polypeptide useful in the method of the invention. A nucleic acid encoding the polypeptide of the invention and an expression vector containing the nucleic acid represent part of the present invention. A non-human host organism and a cell transformed to be used in the method of producing α santalene are also part of the present invention.

Abstract translation: 本发明提供了通过使至少一种多肽与法呢基磷酸（fpp）接触来生产α-檀香草的方法。 特别地，该方法可以在体外或体内进行以产生α-香豆素，在香料和香料领域中非常有用的化合物。 本发明还提供了可用于本发明方法的多肽的氨基酸序列。 编码本发明多肽的核酸和含有核酸的表达载体代表本发明的一部分。 非人宿主生物体和转化用于生产α-檀香烃的方法的细胞也是本发明的一部分。

公开(公告)号：US20150099283A1

公开(公告)日：2015-04-09

申请号：US14577475

申请日：2014-12-19

Applicant: FIRMENICH SA

Inventor： Michel SCHALK ,  Fabienne DEGUERRY

IPC: C12P15/00 ,  C12N9/88 ,  C12P5/00

CPC classification number: C12P15/00 ,  C12N9/88 ,  C12N15/8243 ,  C12P5/002 ,  C12Y402/0307

Abstract: A method of producing patchoulol and 7-epi-α-selinene by contacting at least one polypeptide with farnesyl phyrophosphate (fpp). The method may be carried out in vitro or in vivo to produce patchoulol and 7-epi-α-selinene, compounds which can be useful in the field of perfumery.

Abstract translation: 通过使至少一种多肽与法呢基磷酸（fpp）接触来生产帕替洛尔和7-表 - α-塞来林的方法。 该方法可以在体外或体内进行，以产生可用于香料领域的化合物，其可用于香草醇和7-表 - α-硒烯。