公开(公告)号：US20150099283A1

公开(公告)日：2015-04-09

申请号：US14577475

申请日：2014-12-19

Applicant: FIRMENICH SA

Inventor： Michel SCHALK ,  Fabienne DEGUERRY

IPC: C12P15/00 ,  C12N9/88 ,  C12P5/00

CPC classification number: C12P15/00 ,  C12N9/88 ,  C12N15/8243 ,  C12P5/002 ,  C12Y402/0307

Abstract: A method of producing patchoulol and 7-epi-α-selinene by contacting at least one polypeptide with farnesyl phyrophosphate (fpp). The method may be carried out in vitro or in vivo to produce patchoulol and 7-epi-α-selinene, compounds which can be useful in the field of perfumery.

Abstract translation: 通过使至少一种多肽与法呢基磷酸（fpp）接触来生产帕替洛尔和7-表 - α-塞来林的方法。 该方法可以在体外或体内进行，以产生可用于香料领域的化合物，其可用于香草醇和7-表 - α-硒烯。

公开(公告)号：US20170283841A1

公开(公告)日：2017-10-05

申请号：US15619658

申请日：2017-06-12

Applicant: FIRMENICH SA

Inventor： Michel SCHALK ,  Fabienne DEGUERRY

IPC: C12P15/00 ,  C12P5/00 ,  C12N9/88 ,  C12N15/82

CPC classification number: C12P15/00 ,  C12N9/88 ,  C12N15/8243 ,  C12P5/002 ,  C12Y402/0307

Abstract: The present invention provides a method of producing patchoulol and 7-epi-α-selinene, said method comprising contacting at least one polypeptide with farnesyl phyrophosphate (FPP). In particular, said method may be carried out in vitro or in vivo to produce patchoulol and 7-epi-α-selinene, compounds which can be useful in the field of perfumery. The present invention also provides the amino acid sequence of a polypeptide useful in the method of the invention. A nucleic acid encoding the polypeptide of the invention and an expression vector containing said nucleic acid are also part of the present invention. A non-human host organism or a cell transformed to be used in the method of producing patchoulol and 7-epi-α-selinene is also an object of the present invention.

公开(公告)号：US20180251797A1

公开(公告)日：2018-09-06

申请号：US15330814

申请日：2015-05-06

Applicant: Firmenich SA

Inventor： Yu-Hua ZHANG ,  Michel SCHALK ,  Olivier HAEFLIGER ,  Xiu-Feng HE ,  Fabienne DEGUERRY

IPC: C12P7/02 ,  C12N9/16 ,  C12N9/02

CPC classification number: C12P7/02 ,  C12N9/0004 ,  C12N9/16 ,  C12P7/04 ,  C12Y301/07007

Abstract: The present invention relates to a method of producing drimenol and/or drimenol derivatives by contacting at least one polypeptide with farnesyl diphosphate. The method may be performed in vitro or in vivo. The present invention also provides amino acid sequences of polypeptides useful in the method of the invention and nucleic acid encoding the polypeptides of the invention. The method further provides host cells or organisms genetically modified to express the polypeptides of the invention and useful to produce drimenol and/or drimenol derivatives.

公开(公告)号：US20180094281A1

公开(公告)日：2018-04-05

申请号：US15564671

申请日：2016-04-11

Applicant: Firmenich SA

Inventor： Michel SCHALK ,  Fabienne DEGUERRY ,  Pan LI ,  Xiufeng HE ,  Qi WANG

IPC: C12P5/00 ,  C12N9/88

CPC classification number: C12P5/007 ,  C12N9/88 ,  C12Y402/03039 ,  C12Y402/03079

Abstract: Provided herein is an isolated polypeptide from Juniperus virginiana, Platycladus orientalis ‘Beverleyensis’ or Platycladus orientalis comprising a (+)-cedrol or a (−)-thujopsene synthase. Further provided herein is an isolated nucleic acid molecule from Juniperus virginiana, Platycladus orientalis ‘Beverleyensis’ or Platycladus orientalis encoding a (+)-cedrol or (−)-thujopsene synthase. Further provided herein are methods of producing (+)-cedrol or (−)-thujopsene.