公开(公告)号：US20190002925A1

公开(公告)日：2019-01-03

申请号：US15737809

申请日：2016-06-30

申请人： Firmenich SA

发明人： Michel SCHALK ,  Letizia ROCCI

IPC分类号： C12P5/00 ,  C12N9/90 ,  C12N9/88 ,  C12N15/70

CPC分类号： C12P5/002 ,  C12N9/88 ,  C12N9/90 ,  C12N15/63 ,  C12N15/70 ,  C12N15/74 ,  C12N15/79 ,  C12P5/007 ,  C12Y402/03 ,  C12Y505/01012 ,  C12Y505/01014

摘要： Provided herein are methods of producing (+)-manool comprising: contacting geranylgeranyl diphosphate with an copalyl diphosphate (CPP) synthase to form a (9S, 10S)-copalyl diphosphate wherein the CPP synthase comprises an amino acid sequence having at least 90%, 95%, 98%, 99% and 100% sequence identity to a polypeptide selected from the group consisting of SEQ ID NO: 1 and SEQ ID NO:2; and contacting the CPP with a sclareol synthase enzyme to form (+)-manool.

公开(公告)号：US20140162332A1

公开(公告)日：2014-06-12

申请号：US14136938

申请日：2013-12-20

申请人： FIRMENICH SA

发明人： Michel SCHALK

IPC分类号： C12P7/02

CPC分类号： C12P7/02 ,  C12N9/16 ,  C12P7/18

摘要： The present invention provides a method of producing sclareol, the method comprising contacting a particular polypeptide having a sclareol synthase activity with labdenediol diphosphate (LPP). In particular, the method may be carried out in vitro or in vivo to produce sclareol, a very useful compound in the fields of perfumery and flavoring. The present invention also provides the amino acid sequence of the polypeptide used in the method. A nucleic acid derived from Salvia sclarea and encoding the polypeptide of the invention, an expression vector containing the nucleic acid, as well as a non-human organism or a cell transformed to harbor the same nucleic acid, are also part of the present invention.

摘要翻译： 本发明提供了生产香紫苏醇的方法，该方法包括使具有香紫苏醇合成酶活性的特定多肽与二氢二苯酚二磷酸（LPP）接触。 特别地，该方法可以在体外或体内进行以产生香紫苏醇，香料和香料领域中非常有用的化合物。 本发明还提供了该方法中使用的多肽的氨基酸序列。 衍生自丹参的核酸并编码本发明的多肽，含有核酸的表达载体以及转化成含有相同核酸的非人生物体或细胞也是本发明的一部分。

公开(公告)号：US20170283841A1

公开(公告)日：2017-10-05

申请号：US15619658

申请日：2017-06-12

申请人： FIRMENICH SA

发明人： Michel SCHALK ,  Fabienne DEGUERRY

IPC分类号： C12P15/00 ,  C12P5/00 ,  C12N9/88 ,  C12N15/82

CPC分类号： C12P15/00 ,  C12N9/88 ,  C12N15/8243 ,  C12P5/002 ,  C12Y402/0307

摘要： The present invention provides a method of producing patchoulol and 7-epi-α-selinene, said method comprising contacting at least one polypeptide with farnesyl phyrophosphate (FPP). In particular, said method may be carried out in vitro or in vivo to produce patchoulol and 7-epi-α-selinene, compounds which can be useful in the field of perfumery. The present invention also provides the amino acid sequence of a polypeptide useful in the method of the invention. A nucleic acid encoding the polypeptide of the invention and an expression vector containing said nucleic acid are also part of the present invention. A non-human host organism or a cell transformed to be used in the method of producing patchoulol and 7-epi-α-selinene is also an object of the present invention.

公开(公告)号：US20160215301A1

公开(公告)日：2016-07-28

申请号：US15023640

申请日：2014-09-19

申请人： FIRMENICH SA

发明人： Laurent DAVIET ,  Letizia ROCCI ,  Michel SCHALK

IPC分类号： C12P5/00 ,  C12N9/02 ,  C12P7/02

CPC分类号： C12P5/007 ,  C12N9/0071 ,  C12N9/0079 ,  C12P7/02 ,  C12Y114/13 ,  C12Y114/14001

摘要： Methods and compositions for producing a sesquiterpene alcohol comprising contacting a sesquiterpene with a p450 polypeptide with monooxygenase activity.

摘要翻译： 包括用于制备倍半萜醇的方法和组合物，包括使倍半萜烯与单加氧酶活性的p450多肽接触。

公开(公告)号：US20180251797A1

公开(公告)日：2018-09-06

申请号：US15330814

申请日：2015-05-06

申请人： Firmenich SA

发明人： Yu-Hua ZHANG ,  Michel SCHALK ,  Olivier HAEFLIGER ,  Xiu-Feng HE ,  Fabienne DEGUERRY

IPC分类号： C12P7/02 ,  C12N9/16 ,  C12N9/02

CPC分类号： C12P7/02 ,  C12N9/0004 ,  C12N9/16 ,  C12P7/04 ,  C12Y301/07007

摘要： The present invention relates to a method of producing drimenol and/or drimenol derivatives by contacting at least one polypeptide with farnesyl diphosphate. The method may be performed in vitro or in vivo. The present invention also provides amino acid sequences of polypeptides useful in the method of the invention and nucleic acid encoding the polypeptides of the invention. The method further provides host cells or organisms genetically modified to express the polypeptides of the invention and useful to produce drimenol and/or drimenol derivatives.

公开(公告)号：US20180105837A1

公开(公告)日：2018-04-19

申请号：US15688956

申请日：2017-08-29

申请人： Firmenich SA

发明人： Michel SCHALK

IPC分类号： C12P5/00 ,  C12N9/88

CPC分类号： C12P5/002 ,  C12N9/88 ,  C12P5/007 ,  C12Y402/03083

摘要： The present invention provides a method of producing β-santalene, said method comprising contacting at least one polypeptide with farnesyl pyrophosphate (FPP). In particular, said method may be carried out in vitro or in vivo to produce β-santalene, a very useful compound in the fields of perfumery and flavoring. The present invention also provides the amino acid sequence of a polypeptide useful in the method of the invention. A nucleic acid encoding the polypeptide of the invention and an expression vector containing said nucleic acid are also part of the present invention. A non-human host organism or a cell transformed to be used in the method of producing β-santalene is also an object of the present invention.

公开(公告)号：US20180094281A1

公开(公告)日：2018-04-05

申请号：US15564671

申请日：2016-04-11

申请人： Firmenich SA

发明人： Michel SCHALK ,  Fabienne DEGUERRY ,  Pan LI ,  Xiufeng HE ,  Qi WANG

IPC分类号： C12P5/00 ,  C12N9/88

CPC分类号： C12P5/007 ,  C12N9/88 ,  C12Y402/03039 ,  C12Y402/03079

摘要： Provided herein is an isolated polypeptide from Juniperus virginiana, Platycladus orientalis ‘Beverleyensis’ or Platycladus orientalis comprising a (+)-cedrol or a (−)-thujopsene synthase. Further provided herein is an isolated nucleic acid molecule from Juniperus virginiana, Platycladus orientalis ‘Beverleyensis’ or Platycladus orientalis encoding a (+)-cedrol or (−)-thujopsene synthase. Further provided herein are methods of producing (+)-cedrol or (−)-thujopsene.

公开(公告)号：US20150176029A1

公开(公告)日：2015-06-25

申请号：US14530651

申请日：2014-10-31

申请人： FIRMENICH SA

发明人： Michel SCHALK

IPC分类号： C12P5/00 ,  C12N9/88

CPC分类号： C12P5/002 ,  C12N9/88 ,  C12P5/007 ,  C12Y402/03083

摘要： The present invention provides a method of producing β-santalene, said method comprising contacting at least one polypeptide with farnesyl pyrophosphate (FPP). In particular, said method may be carried out in vitro or in vivo to produce β-santalene, a very useful compound in the fields of perfumery and flavoring. The present invention also provides the amino acid sequence of a polypeptide useful in the method of the invention. A nucleic acid encoding the polypeptide of the invention and an expression vector containing said nucleic acid are also part of the present invention. A non-human host organism or a cell transformed to be used in the method of producing β-santalene is also an object of the present invention.

摘要翻译： 本发明提供了一种生产皂苷的方法，所述方法包括使至少一种多肽与焦亚磷酸法呢基（FPP）接触。 特别地，所述方法可以在体外或体内进行以产生在香料和香料领域中非常有用的化合物。 本发明还提供了可用于本发明方法的多肽的氨基酸序列。 编码本发明多肽的核酸和含有该核酸的表达载体也是本发明的一部分。 非人类宿主生物体或转化用于生产ant al烯的方法的细胞也是本发明的目的。

公开(公告)号：US20200308612A1

公开(公告)日：2020-10-01

申请号：US16308890

申请日：2017-07-19

申请人： Firmenich SA

发明人： Michel SCHALK ,  Letizia ROCCI

IPC分类号： C12P7/62 ,  C12P5/00 ,  C12N9/88 ,  C12N9/02 ,  C12N15/70 ,  C12P7/22 ,  C12P7/26

摘要： Provided is a method of producing one or more sesquiterpene compounds comprising: contacting an acyclic FPP precursor with a polypeptide having terpene synthase activity, wherein the polypeptide comprises an amino acid sequence that has at least 55% sequence identity to SEQ ID NO: 1, to produce one or more terpenes selected from the group consisting of isovalencene, spirovetiva-1(10),7(11)-diene and valencene or derivatives thereof, or mixture of sesquiterpenes comprising one or more of isovalencene, spirovetiva-1(10),7(11)-diene and/or valencene; and optionally isolating the one or more terpenes or the mixture. Also described is a nucleic acid derived from Vetiveria zizanoides encoding a polypeptide having sesquiterpene synthase activity, a polypeptide that can be used to produce one or more sesquiterpenes or a mixture of sesquiterpenes comprising one or more of isovalencene, spirovetiva-1(10),7(11)-diene and/or valencene, and a non-human organism or cell comprising the nucleic acid or comprising an expression vector comprising the nucleic acid.

公开(公告)号：US20180208946A1

公开(公告)日：2018-07-26

申请号：US15877183

申请日：2018-01-22

申请人： FIRMENICH SA

发明人： Laurent DAVIET ,  Letizia ROCCI ,  Michel SCHALK

IPC分类号： C12P5/00 ,  C12N9/02 ,  C12P7/02

CPC分类号： C12P5/007 ,  C12N9/0071 ,  C12N9/0079 ,  C12P7/02 ,  C12Y114/13 ,  C12Y114/14001

摘要： This invention relates generally to methods and compositions for producing a sesquiterpene alcohol comprising contacting a sesquiterpene with a P450 polypeptide with monooxygenase activity.