METHOD FOR PRODUCING SCLAREOL
    3.
    发明申请
    METHOD FOR PRODUCING SCLAREOL 审中-公开
    生产SCLAREOL的方法

    公开(公告)号:US20140162332A1

    公开(公告)日:2014-06-12

    申请号:US14136938

    申请日:2013-12-20

    Applicant: FIRMENICH SA

    Inventor: Michel SCHALK

    CPC classification number: C12P7/02 C12N9/16 C12P7/18

    Abstract: The present invention provides a method of producing sclareol, the method comprising contacting a particular polypeptide having a sclareol synthase activity with labdenediol diphosphate (LPP). In particular, the method may be carried out in vitro or in vivo to produce sclareol, a very useful compound in the fields of perfumery and flavoring. The present invention also provides the amino acid sequence of the polypeptide used in the method. A nucleic acid derived from Salvia sclarea and encoding the polypeptide of the invention, an expression vector containing the nucleic acid, as well as a non-human organism or a cell transformed to harbor the same nucleic acid, are also part of the present invention.

    Abstract translation: 本发明提供了生产香紫苏醇的方法,该方法包括使具有香紫苏醇合成酶活性的特定多肽与二氢二苯酚二磷酸(LPP)接触。 特别地,该方法可以在体外或体内进行以产生香紫苏醇,香料和香料领域中非常有用的化合物。 本发明还提供了该方法中使用的多肽的氨基酸序列。 衍生自丹参的核酸并编码本发明的多肽,含有核酸的表达载体以及转化成含有相同核酸的非人生物体或细胞也是本发明的一部分。

    METHOD FOR PRODUCING PATCHOULOL AND 7-EPI-ALPHA-SELINENE

    公开(公告)号:US20170283841A1

    公开(公告)日:2017-10-05

    申请号:US15619658

    申请日:2017-06-12

    Applicant: FIRMENICH SA

    Abstract: The present invention provides a method of producing patchoulol and 7-epi-α-selinene, said method comprising contacting at least one polypeptide with farnesyl phyrophosphate (FPP). In particular, said method may be carried out in vitro or in vivo to produce patchoulol and 7-epi-α-selinene, compounds which can be useful in the field of perfumery. The present invention also provides the amino acid sequence of a polypeptide useful in the method of the invention. A nucleic acid encoding the polypeptide of the invention and an expression vector containing said nucleic acid are also part of the present invention. A non-human host organism or a cell transformed to be used in the method of producing patchoulol and 7-epi-α-selinene is also an object of the present invention.

    METHOD FOR PRODUCING BETA-SANTALENE
    7.
    发明申请

    公开(公告)号:US20180105837A1

    公开(公告)日:2018-04-19

    申请号:US15688956

    申请日:2017-08-29

    Applicant: Firmenich SA

    Inventor: Michel SCHALK

    CPC classification number: C12P5/002 C12N9/88 C12P5/007 C12Y402/03083

    Abstract: The present invention provides a method of producing β-santalene, said method comprising contacting at least one polypeptide with farnesyl pyrophosphate (FPP). In particular, said method may be carried out in vitro or in vivo to produce β-santalene, a very useful compound in the fields of perfumery and flavoring. The present invention also provides the amino acid sequence of a polypeptide useful in the method of the invention. A nucleic acid encoding the polypeptide of the invention and an expression vector containing said nucleic acid are also part of the present invention. A non-human host organism or a cell transformed to be used in the method of producing β-santalene is also an object of the present invention.

    METHOD FOR PRODUCING BETA-SANTALENE
    9.
    发明申请
    METHOD FOR PRODUCING BETA-SANTALENE 审中-公开
    生产苯乙烯的方法

    公开(公告)号:US20150176029A1

    公开(公告)日:2015-06-25

    申请号:US14530651

    申请日:2014-10-31

    Applicant: FIRMENICH SA

    Inventor: Michel SCHALK

    CPC classification number: C12P5/002 C12N9/88 C12P5/007 C12Y402/03083

    Abstract: The present invention provides a method of producing β-santalene, said method comprising contacting at least one polypeptide with farnesyl pyrophosphate (FPP). In particular, said method may be carried out in vitro or in vivo to produce β-santalene, a very useful compound in the fields of perfumery and flavoring. The present invention also provides the amino acid sequence of a polypeptide useful in the method of the invention. A nucleic acid encoding the polypeptide of the invention and an expression vector containing said nucleic acid are also part of the present invention. A non-human host organism or a cell transformed to be used in the method of producing β-santalene is also an object of the present invention.

    Abstract translation: 本发明提供了一种生产皂苷的方法,所述方法包括使至少一种多肽与焦亚磷酸法呢基(FPP)接触。 特别地,所述方法可以在体外或体内进行以产生在香料和香料领域中非常有用的化合物。 本发明还提供了可用于本发明方法的多肽的氨基酸序列。 编码本发明多肽的核酸和含有该核酸的表达载体也是本发明的一部分。 非人类宿主生物体或转化用于生产ant al烯的方法的细胞也是本发明的目的。

    NOVEL POLYPEPTIDES FOR PRODUCING ALBICANOL AND/OR DRIMENOL COMPOUNDS

    公开(公告)号:US20240376502A1

    公开(公告)日:2024-11-14

    申请号:US17760705

    申请日:2020-11-25

    Applicant: FIRMENICH SA

    Abstract: Described herein are plant-derived haloacid dehalogenase-like (HAD-like) polypeptides having cyclic terpene synthase (TPS) activity, in particular suitable for use in biochemical methods of producing drimane-type sesquiterpenes, encompassing drimenol and/or albicanol and related compounds, like phosphorylated drimane-type sesquiterpene alcohols, in particular phosphorylated drimenol and/or albicanol compounds and derivatives. Also described herein are the coding nucleotide sequences of the TPS activity, corresponding expression constructs, recombinant hosts, and methods of preparing such polypeptides and mutants and variants thereof. Also described herein is a method of using such polypeptides, the method including using the polypeptides in the production of odorants, flavours and fragrance ingredients.

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