公开(公告)号：US20190002925A1

公开(公告)日：2019-01-03

申请号：US15737809

申请日：2016-06-30

Applicant: Firmenich SA

Inventor： Michel SCHALK ,  Letizia ROCCI

IPC: C12P5/00 ,  C12N9/90 ,  C12N9/88 ,  C12N15/70

CPC classification number: C12P5/002 ,  C12N9/88 ,  C12N9/90 ,  C12N15/63 ,  C12N15/70 ,  C12N15/74 ,  C12N15/79 ,  C12P5/007 ,  C12Y402/03 ,  C12Y505/01012 ,  C12Y505/01014

Abstract: Provided herein are methods of producing (+)-manool comprising: contacting geranylgeranyl diphosphate with an copalyl diphosphate (CPP) synthase to form a (9S, 10S)-copalyl diphosphate wherein the CPP synthase comprises an amino acid sequence having at least 90%, 95%, 98%, 99% and 100% sequence identity to a polypeptide selected from the group consisting of SEQ ID NO: 1 and SEQ ID NO:2; and contacting the CPP with a sclareol synthase enzyme to form (+)-manool.

公开(公告)号：US20160215301A1

公开(公告)日：2016-07-28

申请号：US15023640

申请日：2014-09-19

Applicant: FIRMENICH SA

Inventor： Laurent DAVIET ,  Letizia ROCCI ,  Michel SCHALK

IPC: C12P5/00 ,  C12N9/02 ,  C12P7/02

CPC classification number: C12P5/007 ,  C12N9/0071 ,  C12N9/0079 ,  C12P7/02 ,  C12Y114/13 ,  C12Y114/14001

Abstract: Methods and compositions for producing a sesquiterpene alcohol comprising contacting a sesquiterpene with a p450 polypeptide with monooxygenase activity.

Abstract translation: 包括用于制备倍半萜醇的方法和组合物，包括使倍半萜烯与单加氧酶活性的p450多肽接触。

公开(公告)号：US20200308612A1

公开(公告)日：2020-10-01

申请号：US16308890

申请日：2017-07-19

Applicant: Firmenich SA

Inventor： Michel SCHALK ,  Letizia ROCCI

IPC: C12P7/62 ,  C12P5/00 ,  C12N9/88 ,  C12N9/02 ,  C12N15/70 ,  C12P7/22 ,  C12P7/26

Abstract: Provided is a method of producing one or more sesquiterpene compounds comprising: contacting an acyclic FPP precursor with a polypeptide having terpene synthase activity, wherein the polypeptide comprises an amino acid sequence that has at least 55% sequence identity to SEQ ID NO: 1, to produce one or more terpenes selected from the group consisting of isovalencene, spirovetiva-1(10),7(11)-diene and valencene or derivatives thereof, or mixture of sesquiterpenes comprising one or more of isovalencene, spirovetiva-1(10),7(11)-diene and/or valencene; and optionally isolating the one or more terpenes or the mixture. Also described is a nucleic acid derived from Vetiveria zizanoides encoding a polypeptide having sesquiterpene synthase activity, a polypeptide that can be used to produce one or more sesquiterpenes or a mixture of sesquiterpenes comprising one or more of isovalencene, spirovetiva-1(10),7(11)-diene and/or valencene, and a non-human organism or cell comprising the nucleic acid or comprising an expression vector comprising the nucleic acid.

公开(公告)号：US20180208946A1

公开(公告)日：2018-07-26

申请号：US15877183

申请日：2018-01-22

Applicant: FIRMENICH SA

Inventor： Laurent DAVIET ,  Letizia ROCCI ,  Michel SCHALK

IPC: C12P5/00 ,  C12N9/02 ,  C12P7/02

CPC classification number: C12P5/007 ,  C12N9/0071 ,  C12N9/0079 ,  C12P7/02 ,  C12Y114/13 ,  C12Y114/14001

Abstract: This invention relates generally to methods and compositions for producing a sesquiterpene alcohol comprising contacting a sesquiterpene with a P450 polypeptide with monooxygenase activity.