公开(公告)号：US10190163B2

公开(公告)日：2019-01-29

申请号：US15055252

申请日：2016-02-26

Applicant: Fluidigm Corporation

Inventor： Carolyn G. Conant ,  Tze Howe Charn ,  Jason A. A. West ,  Xiaohui Wang

IPC: C12Q1/68 ,  C12Q1/6874 ,  C12Q1/6844 ,  B01L3/00

Abstract: Described herein are cell-based analytic methods, including a method of incorporating nucleic acid sequences into reaction products from a cell population, wherein the nucleic acid sequences are incorporated into the reaction products of each cell individually or in small groups of cells individually. Also described herein is a matrix-type microfluidic device that permits at least two reagents to be delivered separately to each cell or group of cells, as well as primer combinations useful in the method and device.

公开(公告)号：US09952126B2

公开(公告)日：2018-04-24

申请号：US13781313

申请日：2013-02-28

Applicant: Fluidigm Corporation

Inventor： Brian Fowler ,  Jake Kimball ,  Myo Thu Maung ,  Andrew May ,  Michael C Norris ,  Dominique Toppani ,  Marc A. Unger ,  Jing Wang ,  Jason A. A. West

IPC: C12P19/34 ,  G01N1/28 ,  C12Q1/68 ,  G01N1/34 ,  G01N15/14 ,  B01L3/00 ,  B01L7/00

CPC classification number: G01N1/28 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0668 ,  B01L2300/0864 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0487 ,  B01L2400/086 ,  B01L2400/088 ,  C12P19/34 ,  C12Q1/6813 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q1/6869 ,  G01N1/34 ,  G01N15/1484 ,  C12Q2565/629

Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases.

公开(公告)号：US20160251714A1

公开(公告)日：2016-09-01

申请号：US15055252

申请日：2016-02-26

Applicant: Fluidigm Corporation

Inventor： Carolyn G. Conant ,  Tze Howe Charn ,  Jason A. A. West ,  Xiaohui Wang

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2300/0609 ,  B01L2300/0893 ,  C12Q1/6844 ,  C12Q2525/179 ,  C12Q2563/179 ,  C12Q2565/629

Abstract: Described herein are cell-based analytic methods, including a method of incorporating nucleic acid sequences into reaction products from a cell population, wherein the nucleic acid sequences are incorporated into the reaction products of each cell individually or in small groups of cells individually. Also described herein is a matrix-type microfluidic device that permits at least two reagents to be delivered separately to each cell or group of cells, as well as primer combinations useful in the method and device.

Abstract translation: 本文描述的是基于细胞的分析方法，包括将核酸序列并入来自细胞群体的反应产物中的方法，其中所述核酸序列单独地或以小组细胞单独引入每个细胞的反应产物中。 本文还描述了允许至少两种试剂分别递送至每个细胞或细胞组的基质型微流体装置，以及在该方法和装置中有用的引物组合。