公开(公告)号：US12129480B2

公开(公告)日：2024-10-29

申请号：US16956942

申请日：2018-12-21

Applicant: GENENTECH, INC.

Inventor： Chi Kin Domingos Ng ,  Yongping Guo Crawford ,  Amy Shen ,  Meixia Zhou ,  Bradley R. Snedecor ,  Shahram Misaghi ,  Albert Eric Gao

IPC: C12N15/90 ,  C07K16/00

CPC classification number: C12N15/907 ,  C07K16/00 ,  C12N15/90 ,  C07K2317/51 ,  C07K2317/515 ,  C12N2800/30 ,  C12N2800/90 ,  C12N2840/00

Abstract: The presently disclosed subject matter relates to targeted integration (TI) host cells suitable for the expression of recombinant proteins, as well as methods of producing and using said TI host cells.

公开(公告)号：US20240240175A1

公开(公告)日：2024-07-18

申请号：US18411697

申请日：2024-01-12

Applicant: GENENTECH, INC.

Inventor： Shahram Misaghi ,  Danming Tang ,  Brad Snedecor

IPC: C12N15/10

CPC classification number: C12N15/1082 ,  C12N15/1055 ,  C12N15/1072

Abstract: The presently disclosed subject matter relates to combinatorial vector cloning and transfection strategies for the generation of targeted integration host cells suitable for the expression of recombinant proteins, as well as targeted integration host cells generated by said strategies and compositions comprising said targeted integration host cells.

公开(公告)号：US20230374497A1

公开(公告)日：2023-11-23

申请号：US18176062

申请日：2023-02-28

Applicant: GENENTECH, INC. ,  HOFFMANN-LA ROCHE INC.

Inventor： Amy SHEN ,  Inn Huam Yuk ,  Peggy Wai No Ko ,  Shahram Misaghi ,  Simon Thomas Auslaender ,  Midori Greenwood-Goodwin ,  Michael Wilson Laird ,  Benedikt Alois Claudius Oswald

IPC: C12N15/10 ,  C12N15/11 ,  C12N9/22 ,  C12N15/90 ,  C12N5/00

CPC classification number: C12N15/1082 ,  C12N15/11 ,  C12N9/22 ,  C12N15/907 ,  C12N5/00 ,  C12N2310/20 ,  C12N2800/80

Abstract: The present disclosure relates to modified mammalian cells having reduced or eliminated expression of certain cellular proteins, CRISPR/Cas9 multiplex knockout strategies for making such cells, and methods of using such cells, e.g., in the context of cell-based therapy or as host cells in the production of a product of interest.

公开(公告)号：US11634836B2

公开(公告)日：2023-04-25

申请号：US17562950

申请日：2021-12-27

Applicant: GENENTECH, INC.

Inventor： Shahram Misaghi ,  Bradley R. Snedecor

IPC: C40B30/06 ,  C07K16/00 ,  C12N15/85 ,  C12N15/90 ,  C40B40/02

Abstract: The presently disclosed subject matter relates to “Randomized Configuration Targeted Integration” (also referred to herein as “Randomized Chain Targeted Integration”) (RCTI) strategies for the generation and identification of host cells capable of expressing recombinant proteins, e.g., monoclonal antibodies, as well as compositions derived from the same, e.g., bispecific antibodies, and other complex format proteins, e.g., membrane protein complexes and other difficult to express molecules.