公开(公告)号：US20190264242A1

公开(公告)日：2019-08-29

申请号：US16137264

申请日：2018-09-20

Applicant: Genomatica, Inc.

Inventor： Anthony P. BURGARD ,  Robin E. OSTERHOUT ,  Jun SUN ,  Priti PHARKYA

IPC: C12P7/42 ,  C12P7/18 ,  C12P7/16 ,  C12P7/52 ,  C12N9/02 ,  C12P7/04

Abstract: A non-naturally occurring microbial organism includes a microbial organism having a reductive TCA or Wood-Ljungdahl pathway in which at least one exogenous nucleic acid encoding these pathway enzymes is expressed in a sufficient amount to enhance carbon flux through acetyl-CoA. A method for enhancing carbon flux through acetyl-CoA includes culturing theses non-naturally occurring microbial organisms under conditions and for a sufficient period of time to produce a product having acetyl-CoA as a building block. Another non-naturally occurring microbial organism includes at least one exogenous nucleic acid encoding an enzyme expressed in a sufficient amount to enhance the availability of reducing equivalents in the presence of carbon monoxide or hydrogen, thereby increasing the yield of redox-limited products via carbohydrate-based carbon feedstock. A method for enhancing the availability of reducing equivalents in the presence of carbon monoxide or hydrogen includes culturing this organism for a sufficient period of time to produce a product.

公开(公告)号：US20160355846A1

公开(公告)日：2016-12-08

申请号：US15148759

申请日：2016-05-06

Applicant: Genomatica, Inc.

Inventor： Stephen J. VAN DIEN ,  Anthony P. BURGARD ,  Robert HASELBECK ,  Catherine J. PUJOL-BAXLEY ,  Wei NIU ,  John D. TRAWICK ,  Harry YIM ,  Mark J. BURK ,  Robin E. OSTERHOUT ,  Jun SUN

IPC: C12P7/18 ,  C12N9/10 ,  C12N15/52 ,  C12N9/16 ,  C12N9/02 ,  C12N9/04 ,  C12N9/88 ,  C12N9/00

CPC classification number: C12P7/18 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/13 ,  C12N9/16 ,  C12N9/88 ,  C12N9/93 ,  C12N15/52

Abstract: The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO) pathway comprising at least one exogenous nucleic acid encoding a BDO pathway enzyme expressed in a sufficient amount to produce BDO and further optimized for expression of BDO. The invention additionally provides methods of using such microbial organisms to produce BDO.

Abstract translation: 本发明提供了包含1,4-丁二醇（BDO）途径的非天然存在的微生物生物，其包含至少一种编码足够量的BDO途径酶的外源核酸以产生BDO并进一步优化用于BDO的表达。 本发明还提供了使用这种微生物生产BDO的方法。

公开(公告)号：US20170044572A1

公开(公告)日：2017-02-16

申请号：US15078437

申请日：2016-03-23

Applicant: Genomatica, Inc.

Inventor： Mark J. BURK ,  Anthony P. BURGARD ,  Jun SUN ,  Robin E. OSTERHOUT ,  Priti PHARKYA

IPC: C12P5/02 ,  C12N15/52

CPC classification number: C12P5/02 ,  C07C1/24 ,  C07C2521/04 ,  C07C2521/12 ,  C07C2521/16 ,  C07C2523/10 ,  C07C2523/28 ,  C07C2523/31 ,  C07C2523/745 ,  C07C2527/167 ,  C07C2527/173 ,  C12N15/52 ,  C12P5/026 ,  C12P7/04 ,  Y02E50/343 ,  C07C11/167

Abstract: The invention provides non-naturally occurring microbial organisms having a butadiene pathway. The invention additionally provides methods of using such organisms to produce butadiene.

Abstract translation: 本发明提供具有丁二烯途径的非天然存在的微生物。 本发明另外提供了使用这些生物体来生产丁二烯的方法。

公开(公告)号：US20160319313A1

公开(公告)日：2016-11-03

申请号：US14954487

申请日：2015-11-30

Applicant: Genomatica, Inc.

Inventor： Jun SUN ,  Mark J. BURK ,  Anthony P. BURGARD ,  Robin E. OSTERHOUT ,  Wei NIU ,  John D. TRAWICK ,  Robert HASELBECK

IPC: C12P17/04 ,  C12N15/52 ,  C12P7/18

CPC classification number: C12P17/04 ,  C12N1/20 ,  C12N15/52 ,  C12P7/18

Abstract: The invention provides non-naturally occurring microbial organisms comprising 1,4-butanediol (14-BDO) and gamma-butyrolactone (GBL) pathways comprising at least one exogenous nucleic acid encoding a 14-BDO and GBL pathway enzyme expressed in a sufficient amount to produce 14-BDO and GBL. The invention additionally provides methods of using such microbial organisms to produce 14-BDO and GBL.

Abstract translation: 本发明提供包含1,4-丁二醇（14-BDO）和γ-丁内酯（GBL）途径的非天然存在的微生物生物，其包含至少一种编码14-BDO和GBL途径酶的外源核酸，其以足够的量表达， 生产14-BDO和GBL。 本发明另外提供了使用这种微生物生物来生产14-BDO和GBL的方法。