公开(公告)号：US20170362565A1

公开(公告)日：2017-12-21

申请号：US15585078

申请日：2017-05-02

申请人： Genomatica, Inc.

发明人： Priti PHARKYA ,  Anthony P. BURGARD ,  Stephen J. VAN DIEN ,  Robin E. OSTERHOUT ,  Mark J. BURK ,  John D. TRAWICK ,  Michael P. KUCHINSKAS ,  Brian STEER

IPC分类号： C12N1/20 ,  C12P7/18 ,  C12N15/63 ,  C12N15/52 ,  C12N9/18 ,  C07C31/20 ,  C08G63/181 ,  C08G63/16 ,  C08G63/06 ,  C08G18/32 ,  C08G18/28 ,  C07C59/01 ,  C12P7/42 ,  C08G69/02

CPC分类号： C12N1/20 ,  C07C31/207 ,  C07C59/01 ,  C08G18/284 ,  C08G18/3206 ,  C08G63/06 ,  C08G63/16 ,  C08G63/181 ,  C08G69/02 ,  C12N9/18 ,  C12N15/52 ,  C12N15/63 ,  C12P7/18 ,  C12P7/42 ,  C12Y301/01 ,  Y02P20/52

摘要： The invention provides non-naturally occurring microbial organisms having a 4-hydroxybutyrate pathway and being capable of producing 4-hydroxybutyrate, wherein the microbial organism comprises one or more genetic modifications. The invention additionally provides methods of producing 4-hydroxybutyrate or related products using the microbial organisms.

公开(公告)号：US20170247709A1

公开(公告)日：2017-08-31

申请号：US15290895

申请日：2016-10-11

申请人： Genomatica, Inc.

发明人： Anthony P. BURGARD ,  Mark J. BURK ,  Robin E. OSTERHOUT ,  Priti PHARKYA ,  Jingyi LI

IPC分类号： C12N15/52 ,  C12P7/18 ,  C12N1/20 ,  C12N1/16 ,  C12N1/14

CPC分类号： C12N15/52 ,  C08G63/16 ,  C12N1/14 ,  C12N1/16 ,  C12N1/20 ,  C12P7/18 ,  C12P19/32

摘要： Provided herein are non-naturally occurring eukaryotic organisms that can be engineered to produce and increase the availability of cytosolic acetyl-CoA. Also provided herein are non-naturally occurring eukaryotic organisms having a 1,3-butanediol (1,3-BDO) pathway. and methods of using such organisms to produce 1,3-BDO.

公开(公告)号：US20170191085A1

公开(公告)日：2017-07-06

申请号：US15325396

申请日：2015-07-02

申请人： Genomatica, Inc.

发明人： Priti PHARKYA ,  Anthony P. BURGARD ,  Mark J. BURK

IPC分类号： C12P5/02 ,  C12N9/02 ,  C12N9/12 ,  C12N9/04 ,  C08F36/14 ,  C12P3/00 ,  C12P7/40 ,  C01B3/00 ,  C08F36/06 ,  C12N15/52 ,  C12N9/10

CPC分类号： C12P5/026 ,  C01B3/00 ,  C08F36/06 ,  C08F36/14 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/0067 ,  C12N9/10 ,  C12N9/12 ,  C12N15/52 ,  C12P3/00 ,  C12P7/40 ,  C12Y101/02007 ,  C12Y101/03013 ,  C12Y102/99003 ,  Y02E50/343 ,  Y02E60/324

摘要： The invention provides non-naturally occurring microbial organisms containing butadiene or 2,4-pentadienoate pathways comprising at least one exogenous nucleic acid encoding a butadiene or 2,4-pentadienoate pathway enzyme expressed in a sufficient amount to produce butadiene or 2,4-pentadienoate. The organism can further contain a hydrogen synthesis pathway. The invention additionally provides methods of using such microbial organisms to produce butadiene or 2,4-pentadienoate by culturing a non-naturally occurring microbial organism containing butadiene or 2,4-pentadienoate pathways as described herein under conditions and for a sufficient period of time to produce butadiene or 2,4-pentadienoate. Hydrogen can be produced together with the production of butadiene or 2,4-pentadienoate.

公开(公告)号：US20170130234A1

公开(公告)日：2017-05-11

申请号：US15184940

申请日：2016-06-16

申请人： Genomatica, Inc.

发明人： Anthony P. BURGARD ,  Priti PHARKYA ,  Robin E. OSTERHOUT

IPC分类号： C12N15/52 ,  C12P7/62 ,  C12P17/10

CPC分类号： C12N15/52 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/80 ,  C12N9/88 ,  C12P7/44 ,  C12P7/46 ,  C12P7/62 ,  C12P13/001 ,  C12P13/005 ,  C12P13/02 ,  C12P17/10 ,  C12Y101/01035 ,  C12Y102/0101 ,  C12Y103/01031 ,  C12Y206/01 ,  C12Y402/01

摘要： The invention provides a non-naturally occurring microbial organism having an adipate, 6-aminocaproic acid or caprolactam pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective adipate, 6-aminocaproic acid or caprolactam pathway. The invention additionally provides a method for producing adipate, 6-aminocaproic acid or caprolactam. The method can include culturing an adipate, 6-aminocaproic acid or caprolactam producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding an adipate, 6-aminocaproic acid or caprolactam pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce adipate, 6-aminocaproic acid or caprolactam.

公开(公告)号：US20170044572A1

公开(公告)日：2017-02-16

申请号：US15078437

申请日：2016-03-23

申请人： Genomatica, Inc.

发明人： Mark J. BURK ,  Anthony P. BURGARD ,  Jun SUN ,  Robin E. OSTERHOUT ,  Priti PHARKYA

IPC分类号： C12P5/02 ,  C12N15/52

CPC分类号： C12P5/02 ,  C07C1/24 ,  C07C2521/04 ,  C07C2521/12 ,  C07C2521/16 ,  C07C2523/10 ,  C07C2523/28 ,  C07C2523/31 ,  C07C2523/745 ,  C07C2527/167 ,  C07C2527/173 ,  C12N15/52 ,  C12P5/026 ,  C12P7/04 ,  Y02E50/343 ,  C07C11/167

摘要： The invention provides non-naturally occurring microbial organisms having a butadiene pathway. The invention additionally provides methods of using such organisms to produce butadiene.

摘要翻译： 本发明提供具有丁二烯途径的非天然存在的微生物。 本发明另外提供了使用这些生物体来生产丁二烯的方法。

公开(公告)号：US20160326553A1

公开(公告)日：2016-11-10

申请号：US15108259

申请日：2014-12-23

申请人： GENOMATICA, INC.

发明人： Anthony P. BURGARD ,  Robin E. OSTERHOUT ,  Stephen J. VAN DIEN ,  Priti PHARKYA ,  Tae Hoon YANG ,  Jungik CHOI

IPC分类号： C12P7/18 ,  C12N9/02 ,  C12N15/52 ,  C12N9/52 ,  C12N9/88 ,  C12N9/16 ,  C12N9/12

CPC分类号： C12P7/18 ,  C07K14/245 ,  C12N1/20 ,  C12N9/0036 ,  C12N9/0057 ,  C12N9/1205 ,  C12N9/16 ,  C12N9/52 ,  C12N9/88 ,  C12N9/93 ,  C12N15/52 ,  C12P7/42 ,  C12Y106/01002 ,  C12Y110/03 ,  C12Y207/0104 ,  C12Y301/00 ,  C12Y301/02 ,  C12Y301/02002 ,  C12Y304/21092 ,  C12Y401/01031 ,  C12Y401/01032

摘要： The invention is directed to a non-naturally occurring microbial organism comprising a first attenuation of a succinyl-CoA synthetase or transferase and at least a second attenuation of a succinyl-CoA converting enzyme or a gene encoding a succinate producing enzyme within a multi-step pathway having a net conversion of succinyl-CoA to succinate.

摘要翻译： 本发明涉及一种非天然存在的微生物生物体，其包含琥珀酰-CoA合成酶或转移酶的第一次衰减，以及琥珀酰-CoA转化酶或编码多步骤中产琥珀酸酶的基因的至少第二次衰减 途径具有琥珀酰-CoA向琥珀酸盐的净转化。

公开(公告)号：US20190300918A1

公开(公告)日：2019-10-03

申请号：US15579118

申请日：2016-06-22

申请人： Genomatica, Inc.

发明人： Robin E. OSTERHOUT ,  Priti PHARKYA

IPC分类号： C12P17/10 ,  C12N15/52 ,  C12P13/00 ,  C12P7/40 ,  C12P7/44 ,  C12P7/18 ,  C12N9/04 ,  C12N9/02 ,  C12P17/08 ,  C12N9/06 ,  C12N9/10 ,  C12N9/16 ,  C12N9/00

摘要： Provided herein are non-naturally occurring microbial organisms having biosynthetic pathways for production of target products and one or more genetic modifications that reduce a byproduct of the biosynthetic pathway. Compositions of target products from such cells and methods of using such cells are provided.

公开(公告)号：US20180155742A1

公开(公告)日：2018-06-07

申请号：US15645880

申请日：2017-07-10

申请人： Genomatica, Inc.

发明人： Mark J. BURK ,  Anthony P. BURGARD ,  Jun SUN ,  Robin E. OSTERHOUT ,  Priti PHARKYA

IPC分类号： C12P5/02 ,  C07C11/16 ,  C08F136/06 ,  C12N15/52

CPC分类号： C12P5/026 ,  C07C11/16 ,  C08F136/06 ,  C12N15/52

摘要： The invention provides non-naturally occurring microbial organisms having a butadiene pathway. The invention additionally provides methods of using such organisms to produce butadiene.

公开(公告)号：US20180135087A1

公开(公告)日：2018-05-17

申请号：US15634726

申请日：2017-06-27

申请人： GENOMATICA, INC.

发明人： Anthony P. BURGARD ,  Robin E. OSTERHOUT ,  Priti PHARKYA ,  Mark J. BURK

IPC分类号： C12P17/08 ,  C12N15/52 ,  C12N5/00 ,  C08G63/08 ,  C08G18/42 ,  C07D313/04 ,  C12N15/70 ,  C12N15/63 ,  C12P17/02

CPC分类号： C12P17/08 ,  C07D313/04 ,  C08G18/4277 ,  C08G63/08 ,  C12N5/0018 ,  C12N15/52 ,  C12N15/63 ,  C12N15/70 ,  C12P17/02

摘要： The invention provides non-naturally occurring microbial organisms containing caprolactone pathways having at least one exogenous nucleic acid encoding a butadiene pathway enzyme expressed in a sufficient amount to produce caprolactone. The invention additionally provides methods of using such microbial organisms to produce caprolactone by culturing a non-naturally occurring microbial organism containing caprolactone pathways as described herein under conditions and for a sufficient period of time to produce caprolactone.

公开(公告)号：US20160376600A1

公开(公告)日：2016-12-29

申请号：US15038922

申请日：2014-11-25

申请人： GENOMATICA, INC.

发明人： Robin E. OSTERHOUT ,  Anthony P. BURGARD ,  Priti PHARKYA ,  Stefan ANDRAE

IPC分类号： C12N15/52 ,  C12N9/04 ,  C12N9/90 ,  C12N9/10 ,  C12P7/04 ,  C12N9/88

CPC分类号： C12N15/52 ,  C12N9/0006 ,  C12N9/1022 ,  C12N9/88 ,  C12N9/90 ,  C12P7/04 ,  C12Y101/01244 ,  C12Y202/01003 ,  C12Y401/02043 ,  C12Y503/01027

摘要： The invention provides non-naturally occurring microbial organisms having a formaldehyde fixation pathway, a formate assimilation pathway, and/or a methanol metabolic pathway in combination with a fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway, wherein the microbial organisms selectively produce a fatty alcohol, fatty aldehyde or fatty acid of a specified length or isopropanol. The microbial organisms provided advantageously enhance the production of substrates and/or pathway intermediates for the production of chain length specific fatty alcohols, fatty aldehydes, fatty acids or isopropanol. In some aspects, the microbial organisms of the invention have select gene disruptions or enzyme attenuations that increase production of fatty alcohols, fatty aldehydes or fatty acids. The invention additionally provides methods of using the above microbial organisms to produce a fatty alcohol, a fatty aldehyde, a fatty acid or isopropanol.

摘要翻译： 本发明提供具有甲醛固定通道，甲酸同化途径和/或与脂肪醇，脂肪醛，脂肪酸或异丙醇途径组合的甲醇代谢途径的非天然存在的微生物生物，其中所述微生物有选择地产生 脂肪醇，脂肪醛或指定长度的脂肪酸或异丙醇。 提供的微生物有利地增强了用于生产链长特异性脂肪醇，脂肪醛，脂肪酸或异丙醇的底物和/或途径中间体的生产。 在一些方面，本发明的微生物有选择性的基因破坏或酶减弱，其增加脂肪醇，脂肪醛或脂肪酸的产生。 本发明另外提供了使用上述微生物生产脂肪醇，脂肪醛，脂肪酸或异丙醇的方法。