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    Gene encoding novel luciferase
    11.
    发明授权
    Gene encoding novel luciferase 有权
    基因编码新型荧光素酶

    公开(公告)号：US07871803B2

    公开(公告)日：2011-01-18

    申请号：US11721032

    申请日：2004-12-09

    申请人： Hiromi Takenaka

    发明人： Hiromi Takenaka

    IPC分类号： C12N15/53 ,  C12N9/02 ,  C12Q1/68 ,  C12Q1/66

    CPC分类号： C12N9/0069 ,  G01N2333/90241

    摘要： The present invention provides genes encoding novel luciferases having at least the properties of: being capable of using coelenterazine as their luminescent substrates; and being capable of being recombinantly expressed in a mammal cell as a host and produced to be secreted to the outside of the host cell. Specifically, the gene encoding novel luciferases according to the present invention is a DNA molecule comprising a nucleotide sequence encoding any of the full-length amino acid sequences of two types of luciferase proteins, luciferase 1 and luciferase 2, from M. pacifica, and is, for example, a gene encoding the following full-length amino acid sequence of the luciferase 1. MMEIQVLFAL ICFALVQANP TENKDDIDIV GVEGKFGTTD60 LETDLFTIVE DMNVISRDTN LANSDADRGK MPGKKLPLEV LIEMEANARK AGCTRGCLIC120 LSKIKCTAKM KVYIPGRCHD YGGDKKTGQA GIVGAIVDIP EISGFKELGP MEQFIAQVDL180 CADCTTGCLK GLANVKCSAL LKKWLPDRCA SFADKIQSEV DNIKGLAGDR210

    摘要翻译： 本发明提供编码新的荧光素酶的基因，其具有以下性质：能够使用肠腔素作为其发光底物; 并且能够在作为宿主的哺乳动物细胞中重组表达并产生分泌到宿主细胞的外部。 具体而言，本发明的编码新的荧光素酶的基因是DNA分子，其包含编码来自太平洋太平洋猩猩的两种荧光素酶蛋白质荧光素酶1和荧光素酶2的全长氨基酸序列的核苷酸序列， 中，例如，编码荧光素酶1. MMEIQVLFAL ICFALVQANP TENKDDIDIV GVEGKFGTTD 60 LETDLFTIVE DMNVISRDTN LANSDADRGK MPGKKLPLEV LIEMEANARK AGCTRGCLIC 120 LSKIKCTAKM KVYIPGRCHD YGGDKKTGQA GIVGAIVDIP EISGFKELGP MEQFIAQVDL 180 CADCTTGCLK GLANVKCSAL LKKWLPDRCA SFADKIQSEV DNIKGLAGDR 210的下面的全长氨基酸序列的基因

    METHOD OF CHANGING FLUORESCENCE WAVELENGTH OF FLUORESCENT PROTEIN
    12.
    发明申请
    METHOD OF CHANGING FLUORESCENCE WAVELENGTH OF FLUORESCENT PROTEIN 有权
    荧光蛋白荧光波长变化的方法

    公开(公告)号：US20090318673A1

    公开(公告)日：2009-12-24

    申请号：US12162460

    申请日：2007-01-25

    申请人： Kyoko Suto ,  Hiromi Takenaka ,  Yasuhiro Takenaka

    发明人： Kyoko Suto ,  Hiromi Takenaka ,  Yasuhiro Takenaka

    IPC分类号： C07K1/107 ,  C07K14/435

    CPC分类号： C07K14/43595

    摘要： The present invention provides: a method of changing the fluorescence wavelength of a GFP-like fluorescent protein from copepod while maintaining recombinant expression efficiency, which comprises identifying a structural factor for determining the fluorescence wavelength thereof in the three-dimensional structure of the protein and modifying amino acid residues associated with the structural factor; and a modified fluorescent protein obtained by applying said method. For example, with regard to a GFP-like fluorescent protein from Chiridius poppei, His52 in an α helix-like secondary structure: PFLLSHCMGYGFYHF (α1 47-61) comprising a fluorescent moiety site GYG is replaced with an aromatic amino acid selected from Phe, Tyr and Trp, so as to cause a red shift of the fluorescent peak wavelength; or it is replaced with Ala, Val, Ile, Leu, Gly, Cys, Met, Ser, Thr, or Asp, Asn, Glu or Gln, so as to cause a blue shift of the fluorescence peak wavelength.

    摘要翻译： 本发明提供了一种在保持重组表达效率的同时维持来自桡足类的GFP样荧光蛋白的荧光波长的方法，其包括鉴定用于测定蛋白质三维结构中的荧光波长的结构因子和修饰 与结构因子相关的氨基酸残基; 和通过应用所述方法获得的修饰的荧光蛋白。 例如，关于来自Chiridius poppei的GFP样荧光蛋白，包含荧光部位部位GYG的α螺旋状二级结构的His52被替换为选自以下的芳香族氨基酸：Phe， Tyr和Trp，以引起荧光峰值波长的红移; 或者被Ala，Val，Ile，Leu，Gly，Cys，Met，Ser，Thr或Asp，Asn，Glu或Gln替代，以使荧光峰值波长发生蓝移。

    Method of changing fluorescence wavelength of fluorescent protein
    13.
    发明授权
    Method of changing fluorescence wavelength of fluorescent protein 有权
    改变荧光蛋白荧光波长的方法

    公开(公告)号：US08278120B2

    公开(公告)日：2012-10-02

    申请号：US12162460

    申请日：2007-01-25

    申请人： Kyoko Suto ,  Hiromi Takenaka ,  Yasuhiro Takenaka

    发明人： Kyoko Suto ,  Hiromi Takenaka ,  Yasuhiro Takenaka

    IPC分类号： G01N33/536 ,  G01N33/533 ,  C07K1/00 ,  C07K14/00 ,  C07K17/00

    CPC分类号： C07K14/43595

    摘要： The present invention provides: a method of changing the fluorescence wavelength of a GFP-like fluorescent protein from copepod while maintaining recombinant expression efficiency, which comprises identifying a structural factor for determining the fluorescence wavelength thereof in the three-dimensional structure of the protein and modifying amino acid residues associated with the structural factor; and a modified fluorescent protein obtained by applying said method. For example, with regard to a GFP-like fluorescent protein from Chiridius poppei, His52 in an α helix-like secondary structure: PFLLSHCMGYGFYHF (α1 47-61) comprising a fluorescent moiety site GYG is replaced with an aromatic amino acid selected from Phe, Tyr and Trp, so as to cause a red shift of the fluorescent peak wavelength; or it is replaced with Ala, Val, Ile, Leu, Gly, Cys, Met, Ser, Thr, or Asp, Asn, Glu or Gln, so as to cause a blue shift of the fluorescence peak wavelength.

    摘要翻译： 本发明提供了一种在保持重组表达效率的同时维持来自桡足类的GFP样荧光蛋白的荧光波长的方法，其包括鉴定用于测定蛋白质三维结构中的荧光波长的结构因子和修饰 与结构因子相关的氨基酸残基; 和通过应用所述方法获得的修饰的荧光蛋白。 例如，关于来自Chiridius poppei的GFP样荧光蛋白，包含荧光部位部位GYG的α螺旋状二级结构的His52被替换为选自Phe的芳香族氨基酸， Tyr和Trp，以引起荧光峰值波长的红移; 或者被Ala，Val，Ile，Leu，Gly，Cys，Met，Ser，Thr或Asp，Asn，Glu或Gln替代，以使荧光峰值波长发生蓝移。