公开(公告)号：US08310754B2

公开(公告)日：2012-11-13

申请号：US12748031

申请日：2010-03-26

申请人： Hisashi Okugawa ,  Naoshi Aikawa ,  Masatoshi Sato

发明人： Hisashi Okugawa ,  Naoshi Aikawa ,  Masatoshi Sato

IPC分类号： G02B21/06 ,  G01J3/30

CPC分类号： G02B21/0076 ,  G02B5/0833 ,  G02B5/285

摘要： The present application has a proposition to provide a highly efficient laser excitation fluorescent microscope. Accordingly, a laser excitation fluorescent microscope of the present application includes a laser light source part radiating at least two types of excitation lights having different wavelengths; a light collecting part collecting the two types of excitation lights on a sample; a high-functional dichroic mirror, disposed between the laser light source part and the light collecting part, reflecting the two types of excitation lights to make the excitation lights incident on the light collecting part, and transmitting two types of fluorescence generated at the sample; and a detecting part detecting light transmitted through the high-functional dichroic mirror, in which an incident angle θ of the excitation lights and the fluorescence to the high-functional dichroic mirror satisfies a formula of 0°

摘要翻译： 本申请提出了一种提供高效激光荧光显微镜的命题。 因此，本申请的激光激发荧光显微镜包括：照射具有不同波长的至少两种激发光的激光光源部; 收集样品上的两种激发光的集光部分; 设置在激光光源部和集光部之间的高分辨率反射镜，反射这两种激发光，使激发光入射到聚光部上，并透射在样品上产生的两种荧光; 以及检测部，其检测透过所述高功能分色镜的光，其中入射角＆ 的激发光和对高功能二向色镜的荧光满足0°<＆amp; t; <45°的公式。

公开(公告)号：US07724365B2

公开(公告)日：2010-05-25

申请号：US12292924

申请日：2008-12-01

申请人： Yoko Fukuda ,  Hisashi Okugawa

发明人： Yoko Fukuda ,  Hisashi Okugawa

IPC分类号： G01N21/64

CPC分类号： G01N21/6458 ,  G01N21/6428 ,  G01N2021/6419 ,  G01N2021/6441 ,  G02B21/0032 ,  G02B21/0076

摘要： A proposition of the present invention is to acquire necessary data without any chasm in an observation of a specimen containing plural kinds of substances of which exciting wavelengths are different. Accordingly, a spectral observation method of the present invention, irradiating light to the specimen containing plural kinds of substances (RFP, GFP, and so on) of which exciting wavelengths are different, and detecting spectra of light emitted from the specimen, the spectral observation method sequentially acquires spectral data of substances from the one of which exciting wavelength is long while switching a wavelength of the irradiated light among respective exciting wavelengths of the plural kinds of substances, and excludes the exciting wavelength of the substance corresponding to the spectral data to be acquired from detection wavelengths of the spectra each when acquiring the spectral data of the spectral data of the respective substances.

摘要翻译： 本发明的目的在于在观察包含多种激发波长不同的物质的样本时获得必要的数据，而没有任何裂缝。 因此，本发明的光谱观察方法，对含有激发波长不同的多种物质（RFP，GFP等）的样本照射光并检测从样品发出的光的光谱，光谱观察 方法顺序地从激光波长较长的物质中获取光谱数据，同时在多种物质的各个激发波长之间切换照射光的波长，并且将与光谱数据对应的物质的激发波长排除为 在获取各物质的光谱数据的光谱数据时从光谱的检测波长中获取每个光谱。

公开(公告)号：US20090116008A1

公开(公告)日：2009-05-07

申请号：US12292924

申请日：2008-12-01

申请人： Yoko Fukuda ,  Hisashi Okugawa

发明人： Yoko Fukuda ,  Hisashi Okugawa

IPC分类号： G01N21/25

CPC分类号： G01N21/6458 ,  G01N21/6428 ,  G01N2021/6419 ,  G01N2021/6441 ,  G02B21/0032 ,  G02B21/0076

摘要： A proposition of the present invention is to acquire necessary data without any chasm in an observation of a specimen containing plural kinds of substances of which exciting wavelengths are different. Accordingly, a spectral observation method of the present invention, irradiating light to the specimen containing plural kinds of substances (RFP, GFP, and so on) of which exciting wavelengths are different, and detecting spectra of light emitted from the specimen, the spectral observation method sequentially acquires spectral data of substances from the one of which exciting wavelength is long while switching a wavelength of the irradiated light among respective exciting wavelengths of the plural kinds of substances, and excludes the exciting wavelength of the substance corresponding to the spectral data to be acquired from detection wavelengths of the spectra each when acquiring the spectral data of the spectral data of the respective substances.

摘要翻译： 本发明的目的在于在观察包含多种激发波长不同的物质的样本时获得必要的数据，而没有任何裂缝。 因此，本发明的光谱观察方法，对含有激发波长不同的多种物质（RFP，GFP等）的样本照射光并检测从样品发出的光的光谱，光谱观察 方法顺序地从激光波长较长的物质中获取光谱数据，同时在多种物质的各个激发波长之间切换照射光的波长，并且将与光谱数据对应的物质的激发波长排除为 在获取各物质的光谱数据的光谱数据时从光谱的检测波长中获取每个光谱。

公开(公告)号：US20090010504A1

公开(公告)日：2009-01-08

申请号：US11817405

申请日：2006-06-20

申请人： Hisashi Okugawa ,  Kensaku Fukumoto

发明人： Hisashi Okugawa ,  Kensaku Fukumoto

IPC分类号： G06K9/00

CPC分类号： G02B21/008

摘要： A confocal microscope apparatus capable of increasing a degree of freedom in varying sectioning resolution while keeping a configuration of a confocal microscope simple. The confocal microscope apparatus includes a confocal microscope capable of detecting two or more microscopic appearances with different sectioning and an arithmetic unit that performs arithmetic operations on data of the two or more microscopic appearances detected by the confocal microscope to create data of a microscopic appearance at sectioning resolution different from that of those microscopic appearances. Even if the number of actually measured microscopic appearances is two, it is possible to vary the diameter of a virtual-confocal diaphragm by performing arithmetic operations thereon, and therefore, an arbitrary sectioning resolution can be realized.

摘要翻译： 能够在保持共焦显微镜的构造的同时，能够提高变化的分割分辨率的自由度的共聚焦显微镜装置。 共焦显微镜装置包括能够检测两个或多个具有不同切片的微观外观的共焦显微镜，以及对由共焦显微镜检测的两个或多个微观外观的数据进行算术运算的算术单元，以在切片处产生微观外观的数据 分辨率与那些微观外观不同。 即使实际测量的微观外观的数量为2，可以通过对其进行算术运算来改变虚拟共焦膜的直径，因此可以实现任意的切片分辨率。

公开(公告)号：US20080291534A1

公开(公告)日：2008-11-27

申请号：US12010712

申请日：2008-01-29

申请人： Hisashi Okugawa

发明人： Hisashi Okugawa

IPC分类号： G02B21/30 ,  G02B21/06

CPC分类号： G02B21/0088 ,  G02B21/26 ,  G02B21/30 ,  G02B21/32

摘要： A microscope system includes a casing in which an optical element is housed, an airtight specimen chamber, and an environment control unit that controls temperature and humidity in the specimen chamber and also controls temperature inside the casing. An upper plate of the specimen chamber is an open/close lid opened and closed when inserting or removing a biological specimen. The casing becomes sealed by a bottom plate of the specimen chamber as the specimen chamber is set on the casing.

公开(公告)号：US20060072190A1

公开(公告)日：2006-04-06

申请号：US11286293

申请日：2005-11-25

申请人： Hisashi Okugawa

发明人： Hisashi Okugawa

IPC分类号： G02B21/00

CPC分类号： G02B21/0088 ,  G02B21/26 ,  G02B21/30 ,  G02B21/32

摘要： A microscope system includes a casing in which an optical element is housed, an airtight specimen chamber, and an environment control unit that controls temperature and humidity in the specimen chamber and also controls temperature inside the casing. An upper plate of the specimen chamber is an open/close lid opened and closed when inserting or removing a biological specimen. The casing becomes sealed by a bottom plate of the specimen chamber as the specimen chamber is set on the casing.

公开(公告)号：US06906859B2

公开(公告)日：2005-06-14

申请号：US10453660

申请日：2003-06-04

申请人： Toshiaki Nihoshi ,  Hisashi Okugawa

发明人： Toshiaki Nihoshi ,  Hisashi Okugawa

IPC分类号： G02B21/06 ,  G02B21/08 ,  G02B21/16

CPC分类号： G02B21/08 ,  G02B21/06 ,  G02B21/16

摘要： An epi-illumination apparatus for fluorescent observation, adjusting light intensities of a plurality of illuminations on a sample over a wide wavelength band continuously and being configured inexpensively, and a fluorescence microscope having the same are provided. A light source, extracting means, an aperture stop, and a filter are arranged on a predetermined optical axis. The aperture stop is arranged on a plane generally conjugated with a pupil plane of an objective. The filter is placed near the aperture stop. The extracting means extracts a plurality of narrow wavelength bands from the wavelength band of the illumination emitted from the light source. The filter has regions of different spectral transmission characteristics to the narrow wavelength bands. The adjusting means for adjusting light intensities of transmitted light from the filter in the narrow wavelength bands independently by moving the filter in a direction orthogonal to the optical axis is provided.

摘要翻译： 一种用于荧光观察的外照明装置，连续地调节宽波长带上的样品上的多个照明的光强度并且被廉价地配置，并且提供具有该照明装置的荧光显微镜。 在预定的光轴上布置有光源，提取装置，孔径光阑和滤光器。 孔径光阑布置在通常与物镜的光瞳平面共轭的平面上。 过滤器放置在孔径光阑附近。 提取装置从从光源发射的照明的波长带中提取多个窄波长带。 滤波器具有与窄波长带不同的频谱传输特性的区域。 提供了一种调节装置，用于通过沿与光轴正交的方向移动滤光器来独立地调节来自窄波长带中的滤光器的透射光的光强度。

公开(公告)号：US20100053736A1

公开(公告)日：2010-03-04

申请号：US12585888

申请日：2009-09-28

申请人： Hisashi Okugawa

发明人： Hisashi Okugawa

IPC分类号： G02B21/36

CPC分类号： G02B21/008 ,  G02B21/0032

摘要： The present application generates an image suitable for collectively observing a whole area or all wavelength components in a viewing field. A confocal microscope apparatus includes a detecting unit being disposed on a collecting location of the collecting optical system, separating incident light into a light from a vicinity of a collecting point on the sample and a light from a peripheral of the vicinity, and detecting each of the lights; and an image generating unit generating an image of the sample by a light signal from the vicinity and a light signal from the peripheral of the vicinity, and setting a ratio of the signal of the light from the vicinity of the collecting point to the signal of the light from the peripheral of the vicinity for each of areas on the image.

摘要翻译： 本申请生成适合于在观察区域中共同观察整个区域或全部波长成分的图像。 共聚焦显微镜装置包括：检测单元，设置在收集光学系统的收集位置，将入射光分离成来自样品上的收集点附近的光和来自周围的周边的光; 灯光; 以及图像生成单元，其通过来自附近的光信号和来自周围的周边的光信号生成样本的图像，并且将来自收集点附近的光的信号的比率设定为 来自图像上每个区域的附近的周边的光。

公开(公告)号：US07557988B2

公开(公告)日：2009-07-07

申请号：US12010712

申请日：2008-01-29

申请人： Hisashi Okugawa

发明人： Hisashi Okugawa

IPC分类号： G02B21/26

CPC分类号： G02B21/0088 ,  G02B21/26 ,  G02B21/30 ,  G02B21/32

摘要： A microscope system includes a casing in which an optical element is housed, an airtight specimen chamber, and an environment control unit that controls temperature and humidity in the specimen chamber and also controls temperature inside the casing. An upper plate of the specimen chamber is an open/close lid opened and closed when inserting or removing a biological specimen. The casing becomes sealed by a bottom plate of the specimen chamber as the specimen chamber is set on the casing.

摘要翻译： 显微镜系统包括容纳光学元件的壳体，气密样本室和控制样品室中的温度和湿度并且还控制壳体内的温度的环境控制单元。 样品室的上板是在插入或移除生物样本时打开和关闭的打开/关闭盖。 当试样室设置在壳体上时，壳体被试样室的底板密封。

公开(公告)号：US20090034062A1

公开(公告)日：2009-02-05

申请号：US11912830

申请日：2006-10-16

申请人： Hisashi Okugawa

发明人： Hisashi Okugawa

IPC分类号： G02B21/00

CPC分类号： G02B21/0048 ,  G02B21/008

摘要： A confocal microscope apparatus is capable of obtaining a high confocal effect while detecting an image of a specimen line by line. The confocal microscope apparatus may include an illuminating optical system which illuminates a line-shaped area on a specimen plane in a specimen by collected light, an image-forming optical system which forms an image of light emitted from the specimen plane, a two-dimensional light detector which is placed at a conjugate plane of the specimen plane, a scanning unit which moves the line-shaped area on the specimen plane, and a correcting unit which corrects a pixel signal of a specific line on the two-dimensional light detector having a confocal relation with the line-shaped area based on a pixel signal of a peripheral line of the specific line.

摘要翻译： 共焦显微镜装置能够在逐行检测样本的图像的同时获得高的共聚焦效果。 共焦显微镜装置可以包括照明光学系统，其通过收集的光照射样品中的样本平面上的线状区域，形成从样本平面发射的光的图像的成像光学系统，二维 放置在检体平面的共轭面的光检测器，移动检体平面上的线状区域的扫描单元，以及校正单元，其对二维光检测器上的特定线的像素信号进行校正，二维光检测器具有 基于特定线的外围线的像素信号与线状区域的共焦关系。