公开(公告)号：US11499192B2

公开(公告)日：2022-11-15

申请号：US16750897

申请日：2020-01-23

Applicant: ILLUMINA, INC.

Inventor： Xi-Jun Chen ,  Yir-Shyuan Wu ,  Tarun Kumar Khurana ,  Liangliang Qiang ,  Andrew J. Price ,  Elisabet Rosas

IPC: C12Q1/6874 ,  C12N15/10

Abstract: An example of a sequencing kit includes a flow cell, an encapsulation matrix precursor composition, and a radical initiator. The flow cell includes a plurality of chambers and primers attached within each of the plurality of chambers. The encapsulation matrix precursor composition consists of a fluid, a monomer or polymer including a radical generating and chain elongating functional group, a radical source, and a crosslinker. The radical initiator is part of the encapsulation matrix precursor composition or is a separate component.

公开(公告)号：US20230272457A1

公开(公告)日：2023-08-31

申请号：US18179026

申请日：2023-03-06

Applicant: ILLUMINA, INC.

Inventor： Boyan Boyanov ,  Liangliang Qiang ,  Kevin L. Gunderson ,  Kay Klausing ,  Lea Pickering ,  Cyril Delattre ,  Tarun Khurana

IPC: C12Q1/6818 ,  C12Q1/6874 ,  C12Q1/6816

CPC classification number: C12Q1/6818 ,  C12Q1/6874 ,  C12Q1/6816 ,  C12Q2533/101 ,  C12Q2535/113 ,  C12Q2535/122 ,  C12Q2563/103 ,  C12Q2563/125

Abstract: Under one aspect, a composition includes a substrate; a first polynucleotide coupled to the substrate; a second polynucleotide hybridized to the first polynucleotide; and a catalyst coupled to a first nucleotide of the second polynucleotide, the catalyst being operable to cause a chemiluminogenic molecule to emit a photon. Under another aspect, a method includes providing a catalyst operable to cause a first chemiluminogenic molecule to emit a photon; providing a substrate; providing a first polynucleotide coupled to the substrate; hybridizing a second polynucleotide to the first polynucleotide; coupling a first quencher to a first nucleotide of the second polynucleotide; and inhibiting, by the first quencher, photon emission by the first chemiluminogenic molecule.

公开(公告)号：US20230063675A1

公开(公告)日：2023-03-02

申请号：US17978130

申请日：2022-10-31

Applicant: ILLUMINA, INC.

Inventor： Xi-Jun Chen ,  Yir-Shyuan Wu ,  Tarun Kumar Khurana ,  Liangliang Qiang ,  Andrew J. Price ,  Elisabet Rosas

IPC: C12Q1/6874 ,  C12N15/10

Abstract: An example of a sequencing kit includes a flow cell and an encapsulation matrix precursor composition. The flow cell includes a plurality of chambers and primers attached within each of the plurality of chambers. The encapsulation matrix precursor composition consists of a fluid and a polymer selected from the group consisting of agar, agarose, alginate, heparin, alginate sulfate, dextran sulfate, hyaluronan, pectin, carrageenan, gelatin, chitosan, cellulose, a collagen polymer, and combinations thereof.

公开(公告)号：US11567060B2

公开(公告)日：2023-01-31

申请号：US16624567

申请日：2018-06-19

Applicant: ILLUMINA, INC.

Inventor： Boyan Boyanov ,  Rohan N. Akolkar ,  Jeffrey S. Fisher ,  Jeffrey G. Mandell ,  Liangliang Qiang ,  Steven M. Barnard

IPC: G01N33/487 ,  C12Q1/6869 ,  G01N27/447

Abstract: Example nanopore sequencers include a cis well, a trans well, and a nanopore fluidically connecting the cis and trans wells. In one example sequencer, a modified electrolyte (including an electrolyte and a cation complexing agent) is present in the cis well, or the trans well, or in the cis and the trans wells. In another example sequencer, a gel state polyelectrolyte is present in the cis well, or the trans well, or in the cis and the trans wells.

公开(公告)号：US10961576B2

公开(公告)日：2021-03-30

申请号：US16707554

申请日：2019-12-09

Applicant: Illumina, Inc.

Inventor： Boyan Boyanov ,  Jeffrey G Mandell ,  Kevin L Gunderson ,  Jingwei Bai ,  Liangliang Qiang ,  Bradley Baas

IPC: C12Q1/6874 ,  G01N33/487 ,  G01N27/447

Abstract: The disclosure provides detection apparatus having one or more nanopores, methods for making apparatus having one or more nanopore and methods for using apparatus having one or more nanopores. Uses include, but are not limited to detection and sequencing of nucleic acids.

公开(公告)号：US20200239954A1

公开(公告)日：2020-07-30

申请号：US16750897

申请日：2020-01-23

Applicant: ILLUMINA, INC.

Inventor： Xi-Jun Chen ,  Yir-Shyuan Wu ,  Tarun Kumar Khurana ,  Liangliang Qiang ,  Andrew J. Price ,  Elisabet Rosas

IPC: C12Q1/6874 ,  C12N15/10

Abstract: An example of a sequencing kit includes a flow cell, an encapsulation matrix precursor composition, and a radical initiator. The flow cell includes a plurality of chambers and primers attached within each of the plurality of chambers. The encapsulation matrix precursor composition consists of a fluid, a monomer or polymer including a radical generating and chain elongating functional group, a radical source, and a crosslinker. The radical initiator is part of the encapsulation matrix precursor composition or is a separate component.

公开(公告)号：US20190383741A1

公开(公告)日：2019-12-19

申请号：US16439635

申请日：2019-06-12

Applicant: Illumina, Inc.

Inventor： Dajun Yuan ,  Liangliang Qiang ,  Minghao Guo

IPC: G01N21/64 ,  C12Q1/6869

Abstract: A device includes a plurality of imaging pixels in a spatial pattern with a formation of features disposed over the pixels. A first and a second feature of the formation of features are disposed over a first pixel. A first luminophore is disposed within or over the first feature. A second luminophore is disposed within or over the second feature. A structured illumination source is to direct at least a portion of first photons in an illumination pattern to the first feature at a first time, and to direct at least a portion of second photons in the illumination pattern to the second feature at a second time. The structured illumination source includes an illumination pattern generator having an illumination pattern generator actuator connected to the illumination pattern generator to cause the illumination pattern to translate or rotate relative to the formation of features.