公开(公告)号：US11585757B2

公开(公告)日：2023-02-21

申请号：US16439635

申请日：2019-06-12

Applicant: Illumina, Inc.

Inventor： Dajun Yuan ,  Liangliang Qiang ,  Minghao Guo

IPC: G01N21/64 ,  G02F1/1335 ,  G02B26/00 ,  C12Q1/6869

Abstract: A device includes a plurality of imaging pixels in a spatial pattern with a formation of features disposed over the pixels. A first and a second feature of the formation of features are disposed over a first pixel. A first luminophore is disposed within or over the first feature. A second luminophore is disposed within or over the second feature. A structured illumination source is to direct at least a portion of first photons in an illumination pattern to the first feature at a first time, and to direct at least a portion of second photons in the illumination pattern to the second feature at a second time. The structured illumination source includes an illumination pattern generator having an illumination pattern generator actuator connected to the illumination pattern generator to cause the illumination pattern to translate or rotate relative to the formation of features.

公开(公告)号：US20200318167A1

公开(公告)日：2020-10-08

申请号：US16832309

申请日：2020-03-27

Applicant: ILLUMINA, INC.

Inventor： Boyan Boyanov ,  Liangliang Qiang ,  Kevin L. Gunderson ,  Kay Klausing ,  Lea Pickering ,  Cyril Delattre ,  Tarun Khurana

IPC: C12Q1/6818 ,  C12Q1/6874 ,  C12Q1/6816

Abstract: Under one aspect, a composition includes a substrate; a first polynucleotide coupled to the substrate; a second polynucleotide hybridized to the first polynucleotide; and a catalyst coupled to a first nucleotide of the second polynucleotide, the catalyst being operable to cause a chemiluminogenic molecule to emit a photon. Under another aspect, a method includes providing a catalyst operable to cause a first chemiluminogenic molecule to emit a photon; providing a substrate; providing a first polynucleotide coupled to the substrate; hybridizing a second polynucleotide to the first polynucleotide; coupling a first quencher to a first nucleotide of the second polynucleotide; and inhibiting, by the first quencher, photon emission by the first chemiluminogenic molecule.

公开(公告)号：US10519499B2

公开(公告)日：2019-12-31

申请号：US15522987

申请日：2015-07-29

Applicant: Illumina, Inc.

Inventor： Boyan Boyanov ,  Jeffrey G Mandell ,  Kevin L Gunderson ,  Jingwei Bai ,  Liangliang Qiang ,  Bradley Baas

IPC: C12Q1/6874 ,  G01N27/447

Abstract: The disclosure provides detection apparatus having one or more nanopores, methods for making apparatus having one or more nanopore and methods for using apparatus having one or more nanopores. Uses include, but are not limited to detection and sequencing of nucleic acids.

公开(公告)号：US20170356030A1

公开(公告)日：2017-12-14

申请号：US15507465

申请日：2015-09-10

Applicant: ILLUMINA, INC.

Inventor： Boyan Boyanov ,  Liangliang Qiang ,  Kevin L. Gunderson ,  Kay Klausing ,  Lea Pickering ,  Cyril De Lattre ,  Tarun Khurana

IPC: C12Q1/68

CPC classification number: C12Q1/6818 ,  C12Q1/6816 ,  C12Q1/6874 ,  C12Q2533/101 ,  C12Q2535/113 ,  C12Q2535/122 ,  C12Q2563/103 ,  C12Q2563/125

Abstract: Under one aspect, a composition includes a substrate; a first polynucleotide coupled to the substrate; a second polynucleotide hybridized to the first polynucleotide; and a catalyst coupled to a first nucleotide of the second polynucleotide, the catalyst being operable to cause a chemiluminogenic molecule to emit a photon. Under another aspect, a method includes providing a catalyst operable to cause a first chemiluminogenic molecule to emit a photon; providing a substrate; providing a first polynucleotide coupled to the substrate; hybridizing a second polynucleotide to the first polynucleotide; coupling a first quencher to a first nucleotide of the second polynucleotide; and inhibiting, by the first quencher, photon emission by the first chemiluminogenic molecule.

公开(公告)号：US20250164399A1

公开(公告)日：2025-05-22

申请号：US19027132

申请日：2025-01-17

Applicant: Illumina, Inc.

Inventor： Tracy Helen Fung ,  Xiuyu Cai ,  Lisa Kwok ,  Hai Tran ,  Kevan Samiee ,  Liangliang Qiang ,  Boyan Boyanov

IPC: G01N21/64 ,  B01L3/00 ,  G01N15/1434 ,  G01N21/77

Abstract: An example sensor includes a flow cell, a detection device, and a controller. The flow cell includes a passivation layer having opposed surfaces and a reaction site at a first of the opposed surfaces. The flow cell also includes a lid operatively connected to the passivation layer to partially define a flow channel between the lid and the reaction site. The detection device is in contact with a second of the opposed surfaces of the passivation layer, and includes an embedded metal layer that is electrically isolated from other detection circuitry of the detection device. The controller is to ground the embedded metal layer.

公开(公告)号：US11680906B2

公开(公告)日：2023-06-20

申请号：US16607354

申请日：2018-04-19

Applicant: ILLUMINA, INC.

Inventor： Tracy Helen Fung ,  Xiuyu Cai ,  Lisa Kwok ,  Hai Tran ,  Kevan Samiee ,  Liangliang Qiang ,  Boyan Boyanov

IPC: G01N21/64 ,  G01N21/77 ,  B01L3/00 ,  G01N15/14

CPC classification number: G01N21/6454 ,  B01L3/502715 ,  G01N15/1436 ,  G01N21/6486 ,  G01N21/7703 ,  G01N2021/7763

Abstract: An example sensor includes a flow cell, a detection device, and a controller. The flow cell includes a passivation layer having opposed surfaces and a reaction site at a first of the opposed surfaces. The flow cell also includes a lid operatively connected to the passivation layer to partially define a flow channel between the lid and the reaction site. The detection device is in contact with a second of the opposed surfaces of the passivation layer, and includes an embedded metal layer that is electrically isolated from other detection circuitry of the detection device. The controller is to ground the embedded metal layer.

公开(公告)号：US11629373B2

公开(公告)日：2023-04-18

申请号：US16832309

申请日：2020-03-27

Applicant: ILLUMINA, INC.

Inventor： Boyan Boyanov ,  Liangliang Qiang ,  Kevin L. Gunderson ,  Kay Klausing ,  Lea Pickering ,  Cyril Delattre ,  Tarun Khurana

IPC: C12Q1/6818 ,  C12Q1/6874 ,  C12Q1/6816

Abstract: Under one aspect, a composition includes a substrate; a first polynucleotide coupled to the substrate; a second polynucleotide hybridized to the first polynucleotide; and a catalyst coupled to a first nucleotide of the second polynucleotide, the catalyst being operable to cause a chemiluminogenic molecule to emit a photon. Under another aspect, a method includes providing a catalyst operable to cause a first chemiluminogenic molecule to emit a photon; providing a substrate; providing a first polynucleotide coupled to the substrate; hybridizing a second polynucleotide to the first polynucleotide; coupling a first quencher to a first nucleotide of the second polynucleotide; and inhibiting, by the first quencher, photon emission by the first chemiluminogenic molecule.

公开(公告)号：US12241835B2

公开(公告)日：2025-03-04

申请号：US18313015

申请日：2023-05-05

Applicant: ILLUMINA, INC.

Inventor： Tracy Helen Fung ,  Xiuyu Cai ,  Lisa Kwok ,  Hai Tran ,  Kevan Samiee ,  Liangliang Qiang ,  Boyan Boyanov

IPC: G01N21/64 ,  B01L3/00 ,  G01N15/1434 ,  G01N21/77

Abstract: An example sensor includes a flow cell, a detection device, and a controller. The flow cell includes a passivation layer having opposed surfaces and a reaction site at a first of the opposed surfaces. The flow cell also includes a lid operatively connected to the passivation layer to partially define a flow channel between the lid and the reaction site. The detection device is in contact with a second of the opposed surfaces of the passivation layer, and includes an embedded metal layer that is electrically isolated from other detection circuitry of the detection device. The controller is to ground the embedded metal layer.

公开(公告)号：US11732301B2

公开(公告)日：2023-08-22

申请号：US17188193

申请日：2021-03-01

Applicant: Illumina, Inc.

Inventor： Boyan Boyanov ,  Jeffrey G Mandell ,  Kevin L Gunderson ,  Jingwei Bai ,  Liangliang Qiang ,  Bradley Baas

IPC: C12Q1/6874 ,  G01N33/487 ,  G01N27/447

CPC classification number: C12Q1/6874 ,  G01N27/44782 ,  G01N27/44791 ,  G01N33/48721

Abstract: The disclosure provides detection apparatus having one or more nanopores, methods for making apparatus having one or more nanopore and methods for using apparatus having one or more nanopores. Uses include, but are not limited to detection and sequencing of nucleic acids.

公开(公告)号：US11535890B2

公开(公告)日：2022-12-27

申请号：US16750897

申请日：2020-01-23

Applicant: ILLUMINA, INC.

Inventor： Xi-Jun Chen ,  Yir-Shyuan Wu ,  Tarun Kumar Khurana ,  Liangliang Qiang ,  Andrew J. Price ,  Elisabet Rosas

IPC: C12Q1/6874 ,  C12N15/10

Abstract: An example of a sequencing kit includes a flow cell, an encapsulation matrix precursor composition, and a radical initiator. The flow cell includes a plurality of chambers and primers attached within each of the plurality of chambers. The encapsulation matrix precursor composition consists of a fluid, a monomer or polymer including a radical generating and chain elongating functional group, a radical source, and a crosslinker. The radical initiator is part of the encapsulation matrix precursor composition or is a separate component.