公开(公告)号：US12227791B2

公开(公告)日：2025-02-18

申请号：US18051836

申请日：2022-11-01

Applicant: ILLUMINA, INC.

Inventor： Raquel Maria Sanches-Kuiper ,  Vincent Peter Smith ,  Sean Humphray

IPC: C12Q1/6806 ,  C12N15/10

Abstract: Embodiments provided herein relate to methods and compositions for preparing nucleic acid libraries. Some embodiments include preparing libraries from nucleic acids obtained from degraded samples, such as ancient samples and fixed samples.

公开(公告)号：US10988797B2

公开(公告)日：2021-04-27

申请号：US16703527

申请日：2019-12-04

Applicant: ILLUMINA, INC.

Inventor： Raquel Maria Sanches-Kuiper ,  Vincent Peter Smith ,  Sean Humphray

IPC: C12Q1/6806 ,  C12N15/10

Abstract: Embodiments provided herein relate to methods and compositions for preparing nucleic acid libraries. Some embodiments include preparing libraries from nucleic acids obtained from degraded samples, such as ancient samples and fixed samples.

公开(公告)号：US20190292583A1

公开(公告)日：2019-09-26

申请号：US16372793

申请日：2019-04-02

Applicant: ILLUMINA, INC.

Inventor： Raquel Maria Sanches-Kuiper ,  Vincent Peter Smith ,  Sean Humphray

IPC: C12Q1/6806 ,  C12N15/10

Abstract: Embodiments provided herein relate to methods and compositions for preparing nucleic acid libraries. Some embodiments include preparing libraries from nucleic acids obtained from degraded samples, such as ancient samples and fixed samples.

公开(公告)号：US10294511B2

公开(公告)日：2019-05-21

申请号：US15029103

申请日：2014-10-16

Applicant: ILLUMINA, INC.

Inventor： Raquel Maria Sanches-Kuiper ,  Vincent Peter Smith ,  Sean Humphray

IPC: C12Q1/68 ,  C12N15/10 ,  C12Q1/6806

Abstract: Embodiments provided herein relate to methods and compositions for preparing nucleic acid libraries. Some embodiments include preparing libraries from nucleic acids obtained from degraded samples, such as ancient samples and fixed samples.

公开(公告)号：US20160265027A1

公开(公告)日：2016-09-15

申请号：US15029103

申请日：2014-10-16

Applicant: ILLUMINA, INC.

Inventor： Raquel Maria Sanches-Kuiper ,  Vincent Peter Smith ,  Sean Humphray

IPC: C12Q1/68 ,  C12N15/10

CPC classification number: C12Q1/6806 ,  C12N15/1093 ,  C12Q2521/301 ,  C12Q2521/531 ,  C12Q2523/303

Abstract: Embodiments provided herein relate to methods and compositions for preparing nucleic acid libraries. Some embodiments include preparing libraries from nucleic acids obtained from degraded samples, such as ancient samples and fixed samples.

Abstract translation: 本文提供的实施方案涉及用于制备核酸文库的方法和组合物。 一些实施方案包括从从降解的样品（例如古代样品和固定样品）获得的核酸制备文库。

公开(公告)号：US20140080721A1

公开(公告)日：2014-03-20

申请号：US13982024

申请日：2012-01-12

Applicant: ILLUMINA, INC.

Inventor： Kay Klausing ,  Min-Jui Richard Shen ,  John Moore ,  Vincent Peter Smith ,  Kevin Hall ,  Niall Anthony Gormley ,  Avgousta Ioannou ,  Epameinondas Fritzilas ,  Roberto Rigatti

IPC: C12N15/10

CPC classification number: C12Q1/6874 ,  C12N15/1003 ,  C12Q1/6832 ,  C12Q1/6869 ,  C40B20/04 ,  C12Q2523/319 ,  C12Q2527/125 ,  C12Q2527/127

Abstract: Provided herein is a method of inhibiting degradation of nucleic acids during a nucleic acid processing step selected from fragmentation and detection comprising contacting the nucleic acids with a solution comprising gallic acid, analogues, derivatives thereof or mixtures thereof, during the processing step, wherein the contacting inhibits degradation of the nucleic acids. Also provided herein is a method of inhibiting light-induced degradation of nucleic acids. Additionally, provided herein is a method of reducing or inhibiting nucleic acid damage during preparation of a nucleic acid sample comprising fragmenting the nucleic acid sequences in the sample in a solution comprising one of more compounds, the compounds inhibiting degradation of the nucleic acid sequences in the sample.

Abstract translation: 本文提供了一种在选择碎片化和检测的核酸加工步骤期间抑制核酸降解的方法，包括在加工步骤期间使核酸与包含没食子酸，其类似物，衍生物或其混合物的溶液接触，其中所述接触 抑制核酸的降解。 本文还提供抑制光诱导的核酸降解的方法。 此外，本文提供的是在制备核酸样品期间减少或抑制核酸损伤的方法，包括在包含一种或多种化合物的溶液中碎片样品中的核酸序列，所述化合物抑制核酸序列的降解 样品。