公开(公告)号：US20140080721A1

公开(公告)日：2014-03-20

申请号：US13982024

申请日：2012-01-12

Applicant: ILLUMINA, INC.

Inventor： Kay Klausing ,  Min-Jui Richard Shen ,  John Moore ,  Vincent Peter Smith ,  Kevin Hall ,  Niall Anthony Gormley ,  Avgousta Ioannou ,  Epameinondas Fritzilas ,  Roberto Rigatti

IPC: C12N15/10

CPC classification number: C12Q1/6874 ,  C12N15/1003 ,  C12Q1/6832 ,  C12Q1/6869 ,  C40B20/04 ,  C12Q2523/319 ,  C12Q2527/125 ,  C12Q2527/127

Abstract: Provided herein is a method of inhibiting degradation of nucleic acids during a nucleic acid processing step selected from fragmentation and detection comprising contacting the nucleic acids with a solution comprising gallic acid, analogues, derivatives thereof or mixtures thereof, during the processing step, wherein the contacting inhibits degradation of the nucleic acids. Also provided herein is a method of inhibiting light-induced degradation of nucleic acids. Additionally, provided herein is a method of reducing or inhibiting nucleic acid damage during preparation of a nucleic acid sample comprising fragmenting the nucleic acid sequences in the sample in a solution comprising one of more compounds, the compounds inhibiting degradation of the nucleic acid sequences in the sample.

Abstract translation: 本文提供了一种在选择碎片化和检测的核酸加工步骤期间抑制核酸降解的方法，包括在加工步骤期间使核酸与包含没食子酸，其类似物，衍生物或其混合物的溶液接触，其中所述接触 抑制核酸的降解。 本文还提供抑制光诱导的核酸降解的方法。 此外，本文提供的是在制备核酸样品期间减少或抑制核酸损伤的方法，包括在包含一种或多种化合物的溶液中碎片样品中的核酸序列，所述化合物抑制核酸序列的降解 样品。

公开(公告)号：US10106851B2

公开(公告)日：2018-10-23

申请号：US15836011

申请日：2017-12-08

Applicant: ILLUMINA, INC.

Inventor： Kay Klausing ,  Min-Jui Richard Shen ,  John Moore ,  Vincent Peter Smith ,  Kevin Hall

IPC: C12Q1/68 ,  C12N15/10 ,  C12Q1/6874 ,  C40B20/04 ,  C12Q1/6832 ,  C12Q1/6869

Abstract: A method of inhibiting light-induced degradation of nucleic acids includes irradiating a portion of the nucleic acids in the presence of a detection solution comprising a polyphenolic compound. A method of detecting a nucleic acid having a fluorescent tag includes irradiating at least a portion of the nucleic acid with light of a suitable wavelength to induce a fluorescence emission and detecting the fluorescence emission. Optionally, the polyphenolic compound is gallic acid, a lower alkyl ester thereof, or mixtures thereof. A kit includes one or more nucleotides, an enzyme capable of catalyzing incorporation of the nucleotides into a nucleic acid strand and a polyphenolic compound suitable for preparing a detection solution.

公开(公告)号：US09856529B2

公开(公告)日：2018-01-02

申请号：US14729376

申请日：2015-06-03

Applicant: Illumina, Inc.

Inventor： Kay Klausing ,  Min-Jui Richard Shen ,  John Moore ,  Vincent Peter Smith ,  Kevin Hall

IPC: C12Q1/68 ,  C40B20/04 ,  C12N15/10 ,  C12Q1/48

CPC classification number: C12Q1/6874 ,  C12N15/1003 ,  C12Q1/6832 ,  C12Q1/6869 ,  C40B20/04 ,  C12Q2523/319 ,  C12Q2527/125 ,  C12Q2527/127

Abstract: A method of inhibiting light-induced degradation of nucleic acids includes irradiating a portion of the nucleic acids in the presence of a detection solution comprising a polyphenolic compound. A method of detecting a nucleic acid having a fluorescent tag includes irradiating at least a portion of the nucleic acid with light of a suitable wavelength to induce a fluorescence emission and detecting the fluorescence emission. Optionally, the polyphenolic compound is gallic acid, a lower alkyl ester thereof, or mixtures thereof. A kit includes one or more nucleotides, an enzyme capable of catalyzing incorporation of the nucleotides into a nucleic acid strand and a polyphenolic compound suitable for preparing a detection solution.

公开(公告)号：US20180100193A1

公开(公告)日：2018-04-12

申请号：US15836011

申请日：2017-12-08

Applicant: ILLUMINA, INC.

Inventor： Kay Klausing ,  Min-Jui Richard Shen ,  John Moore ,  Vincent Peter Smith ,  Kevin Hall

IPC: C40B20/04 ,  C12N15/10

CPC classification number: C12Q1/6874 ,  C12N15/1003 ,  C12Q1/6832 ,  C12Q1/6869 ,  C40B20/04 ,  C12Q2523/319 ,  C12Q2527/125 ,  C12Q2527/127

Abstract: A method of inhibiting light-induced degradation of nucleic acids includes irradiating a portion of the nucleic acids in the presence of a detection solution comprising a polyphenolic compound. A method of detecting a nucleic acid having a fluorescent tag includes irradiating at least a portion of the nucleic acid with light of a suitable wavelength to induce a fluorescence emission and detecting the fluorescence emission. Optionally, the polyphenolic compound is gallic acid, a lower alkyl ester thereof, or mixtures thereof. A kit includes one or more nucleotides, an enzyme capable of catalyzing incorporation of the nucleotides into a nucleic acid strand and a polyphenolic compound suitable for preparing a detection solution.

公开(公告)号：US20150259737A1

公开(公告)日：2015-09-17

申请号：US14729376

申请日：2015-06-03

Applicant: Illumina, Inc.

Inventor： Kay Klausing ,  Min-Jui Richard Shen ,  John Moore ,  Vincent Peter Smith ,  Kevin Hall

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12N15/1003 ,  C12Q1/6832 ,  C12Q1/6869 ,  C40B20/04 ,  C12Q2523/319 ,  C12Q2527/125 ,  C12Q2527/127

Abstract: A method of inhibiting light-induced degradation of nucleic acids includes irradiating a portion of the nucleic acids in the presence of a detection solution comprising a polyphenolic compound. A method of detecting a nucleic acid having a fluorescent tag includes irradiating at least a portion of the nucleic acid with light of a suitable wavelength to induce a fluorescence emission and detecting the fluorescence emission. Optionally, the polyphenolic compound is gallic acid, a lower alkyl ester thereof, or mixtures thereof. A kit includes one or more nucleotides, an enzyme capable of catalyzing incorporation of the nucleotides into a nucleic acid strand and a polyphenolic compound suitable for preparing a detection solution.

Abstract translation: 抑制光诱导的核酸降解的方法包括在存在包含多酚化合物的检测溶液的情况下照射一部分核酸。 检测具有荧光标签的核酸的方法包括用合适波长的光照射核酸的至少一部分以诱导荧光发射并检测荧光发射。 任选地，多酚化合物是没食子酸，其低级烷基酯或其混合物。 试剂盒包括一个或多个核苷酸，能够催化将核苷酸掺入核酸链的酶和适于制备检测溶液的多酚化合物。