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    METHODS FOR SELECTING AND AMPLIFYING POLYNUCLEOTIDES
    1.
    发明申请
    METHODS FOR SELECTING AND AMPLIFYING POLYNUCLEOTIDES 审中-公开

    公开(公告)号：US20170283794A1

    公开(公告)日：2017-10-05

    申请号：US15489549

    申请日：2017-04-17

    Applicant: Illumina, Inc.

    Inventor： Andrea Sabot ,  Roberto Rigatti ,  Min-Jui Richard Shen

    IPC: C12N15/10 ,  C12Q1/68

    CPC classification number: C12N15/1068 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/6874 ,  C12Q2565/543 ,  C12Q2565/507 ,  C12Q2531/107

    Abstract: The invention provides methods for generating a nucleic acid library. Target-specific primer-probes are hybridized to a target nucleic acid fragment to create a hybridization product. Each of the target-specific primer-probes comprises a target-specific sequence and a first adaptor sequence. The target nucleic acid fragment comprises a target genomic region of interest, wherein said target genomic region of interest comprises an exon of a gene and the target-specific primer-probes are tiled across the exon of the gene. The target nucleic acid fragment further comprises a second adaptor sequence different from said first adaptor sequence. Following hybridization, the target-specific primer-probes are extended to create double-stranded extension products and further amplified, thereby generating the nucleic acid library.

    METHOD FOR SELECTING AND AMPLIFYING POLYNUCLEOTIDES
    2.
    发明申请
    METHOD FOR SELECTING AND AMPLIFYING POLYNUCLEOTIDES 有权

    公开(公告)号：US20220064632A1

    公开(公告)日：2022-03-03

    申请号：US17470284

    申请日：2021-09-09

    Applicant: Illumina, Inc.

    Inventor： Andrea Sabot ,  Roberto Rigatti ,  Min-Jui Richard Shen

    IPC: C12N15/10 ,  C12Q1/6837 ,  C12Q1/6874 ,  C12Q1/6806

    Abstract: The invention provides methods for controlling the density of different molecular species on the surface of a solid support. A first mixture of different molecular species is attached to a solid support under conditions to attach each species at a desired density, thereby producing a derivatized support having attached capture molecules. The derivatized support is treated with a second mixture of different molecular species, wherein different molecular species in the second mixture bind specifically to the different capture molecules attached to the solid support. One or more of the capture molecules can be reversibly modified such that the capture molecules have a different activity before and after the second mixture of molecular species are attached. In particular embodiments, the different molecular species are nucleic acids that are reversibly modified to have different activity in an amplification reaction.

    Polymerases
    4.
    发明申请
    Polymerases 有权
    聚合酶

    公开(公告)号：US20140287415A1

    公开(公告)日：2014-09-25

    申请号：US14137434

    申请日：2013-12-20

    Applicant: Illumina, Inc.

    Inventor： Geoffrey Paul Smith ,  Roberto Rigatti ,  Tobias William Barr Ost ,  Shankar Balasubramanian ,  Raquel Maria Sanches-Kuiper

    IPC: C12Q1/68

    CPC classification number: C12N9/1252 ,  C12Q1/686 ,  C12Y207/07007

    Abstract: Modified DNA polymerases have an affinity for DNA such that the polymerase has an ability to incorporate one or more nucleotides into a plurality of separate DNA templates in each reaction cycle. The polymerases are capable of forming an increased number of productive polymerase-DNA complexes in each reaction cycle. The modified polymerases may be used in a number of DNA sequencing applications, especially in the context of clustered arrays.

    Abstract translation: 修饰的DNA聚合酶对DNA具有亲和力，使得聚合酶具有在每个反应循环中将一个或多个核苷酸并入到多个单独的DNA模板中的能力。 聚合酶能够在每个反应循环中形成增加数量的生产性聚合酶-DNA复合物。 修饰的聚合酶可用于许多DNA测序应用，特别是在簇阵列的上下文中。

    METHODS FOR REDUCING NUCLEIC ACID DAMAGE
    5.
    发明申请
    METHODS FOR REDUCING NUCLEIC ACID DAMAGE 审中-公开
    降低核酸损伤的方法

    公开(公告)号：US20140080721A1

    公开(公告)日：2014-03-20

    申请号：US13982024

    申请日：2012-01-12

    Applicant: ILLUMINA, INC.

    Inventor： Kay Klausing ,  Min-Jui Richard Shen ,  John Moore ,  Vincent Peter Smith ,  Kevin Hall ,  Niall Anthony Gormley ,  Avgousta Ioannou ,  Epameinondas Fritzilas ,  Roberto Rigatti

    IPC: C12N15/10

    CPC classification number: C12Q1/6874 ,  C12N15/1003 ,  C12Q1/6832 ,  C12Q1/6869 ,  C40B20/04 ,  C12Q2523/319 ,  C12Q2527/125 ,  C12Q2527/127

    Abstract: Provided herein is a method of inhibiting degradation of nucleic acids during a nucleic acid processing step selected from fragmentation and detection comprising contacting the nucleic acids with a solution comprising gallic acid, analogues, derivatives thereof or mixtures thereof, during the processing step, wherein the contacting inhibits degradation of the nucleic acids. Also provided herein is a method of inhibiting light-induced degradation of nucleic acids. Additionally, provided herein is a method of reducing or inhibiting nucleic acid damage during preparation of a nucleic acid sample comprising fragmenting the nucleic acid sequences in the sample in a solution comprising one of more compounds, the compounds inhibiting degradation of the nucleic acid sequences in the sample.

    Abstract translation: 本文提供了一种在选择碎片化和检测的核酸加工步骤期间抑制核酸降解的方法，包括在加工步骤期间使核酸与包含没食子酸，其类似物，衍生物或其混合物的溶液接触，其中所述接触 抑制核酸的降解。 本文还提供抑制光诱导的核酸降解的方法。 此外，本文提供的是在制备核酸样品期间减少或抑制核酸损伤的方法，包括在包含一种或多种化合物的溶液中碎片样品中的核酸序列，所述化合物抑制核酸序列的降解 样品。

    Method for selecting and amplifying polynucleotides
    6.
    发明授权
    Method for selecting and amplifying polynucleotides 有权

    公开(公告)号：US12060554B2

    公开(公告)日：2024-08-13

    申请号：US17470284

    申请日：2021-09-09

    Applicant: Illumina, Inc.

    Inventor： Andrea Sabot ,  Roberto Rigatti ,  Min-Jui Richard Shen

    IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/6874

    CPC classification number: C12N15/1068 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/6874

    Abstract: The invention provides methods for controlling the density of different molecular species on the surface of a solid support. A first mixture of different molecular species is attached to a solid support under conditions to attach each species at a desired density, thereby producing a derivatized support having attached capture molecules. The derivatized support is treated with a second mixture of different molecular species, wherein different molecular species in the second mixture bind specifically to the different capture molecules attached to the solid support. One or more of the capture molecules can be reversibly modified such that the capture molecules have a different activity before and after the second mixture of molecular species are attached. In particular embodiments, the different molecular species are nucleic acids that are reversibly modified to have different activity in an amplification reaction.

    METHOD FOR SELECTING AND AMPLIFYING POLYNUCLEOTIDES
    10.
    发明申请
    METHOD FOR SELECTING AND AMPLIFYING POLYNUCLEOTIDES 审中-公开

    公开(公告)号：US20200239875A1

    公开(公告)日：2020-07-30

    申请号：US16786198

    申请日：2020-02-10

    Applicant: Illumina, Inc.

    Inventor： Andrea Sabot ,  Roberto Rigatti ,  Min-Jui Richard Shen

    IPC: C12N15/10 ,  C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6837

    Abstract: The invention provides methods for controlling the density of different molecular species on the surface of a solid support. A first mixture of different molecular species is attached to a solid support under conditions to attach each species at a desired density, thereby producing a derivatized support having attached capture molecules. The derivatized support is treated with a second mixture of different molecular species, wherein different molecular species in the second mixture bind specifically to the different capture molecules attached to the solid support. One or more of the capture molecules can be reversibly modified such that the capture molecules have a different activity before and after the second mixture of molecular species are attached. In particular embodiments, the different molecular species are nucleic acids that are reversibly modified to have different activity in an amplification reaction.

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