公开(公告)号：US20210230689A1

公开(公告)日：2021-07-29

申请号：US17227639

申请日：2021-04-12

Applicant: Illumina, Inc. ,  Illumina Cambridge Limited

Inventor： Eric Vermaas ,  Mahdieh Khosroheidari ,  Angela Kalbande ,  Vincent Peter Smith ,  Claire Bevis-Mott ,  Jonathan Mark Boutell ,  Michael Chesney

IPC: C12Q1/6874 ,  C12Q1/6809 ,  C12Q1/6816 ,  C12Q1/6834 ,  C12Q1/6855 ,  C12Q1/6806

Abstract: The present invention is concerned with compositions and methods for improving the rate of correct sample identification in indexed nucleic acid library preparations for multiplex next generation sequencing by blocking the 3′ ends of pooled indexed polynucleotides from multiple samples prior to amplification and sequencing, by exonuclease treatment and optionally blocking the 3′ ends of pooled indexed polynucleotides from multiple samples prior to amplification and sequencing, by exonuclease treatment after protective adapters are ligated to target polynucleotides to degrade unincorporated adapters prior to amplification and sequencing, and/or by modifying or blocking 5′ and 3′ ends of pooled indexed polynucleotides from multiple samples, with an optional exonuclease treatment, prior to amplification and sequencing.

公开(公告)号：US09856529B2

公开(公告)日：2018-01-02

申请号：US14729376

申请日：2015-06-03

Applicant: Illumina, Inc.

Inventor： Kay Klausing ,  Min-Jui Richard Shen ,  John Moore ,  Vincent Peter Smith ,  Kevin Hall

IPC: C12Q1/68 ,  C40B20/04 ,  C12N15/10 ,  C12Q1/48

CPC classification number: C12Q1/6874 ,  C12N15/1003 ,  C12Q1/6832 ,  C12Q1/6869 ,  C40B20/04 ,  C12Q2523/319 ,  C12Q2527/125 ,  C12Q2527/127

Abstract: A method of inhibiting light-induced degradation of nucleic acids includes irradiating a portion of the nucleic acids in the presence of a detection solution comprising a polyphenolic compound. A method of detecting a nucleic acid having a fluorescent tag includes irradiating at least a portion of the nucleic acid with light of a suitable wavelength to induce a fluorescence emission and detecting the fluorescence emission. Optionally, the polyphenolic compound is gallic acid, a lower alkyl ester thereof, or mixtures thereof. A kit includes one or more nucleotides, an enzyme capable of catalyzing incorporation of the nucleotides into a nucleic acid strand and a polyphenolic compound suitable for preparing a detection solution.

公开(公告)号：US20200181687A1

公开(公告)日：2020-06-11

申请号：US16703527

申请日：2019-12-04

Applicant: ILLUMINA, INC.

Inventor： Raquel Maria Sanches-Kuiper ,  Vincent Peter Smith ,  Sean Humphray

IPC: C12Q1/6806 ,  C12N15/10

Abstract: Embodiments provided herein relate to methods and compositions for preparing nucleic acid libraries. Some embodiments include preparing libraries from nucleic acids obtained from degraded samples, such as ancient samples and fixed samples.

公开(公告)号：US11512340B2

公开(公告)日：2022-11-29

申请号：US17068062

申请日：2020-10-12

Applicant: ILLUMINA, INC.

Inventor： Raquel Maria Sanches-Kuiper ,  Vincent Peter Smith ,  Sean Humphray

IPC: C12Q1/6806 ,  C12N15/10

Abstract: Embodiments provided herein relate to methods and compositions for preparing nucleic acid libraries. Some embodiments include preparing libraries from nucleic acids obtained from degraded samples, such as ancient samples and fixed samples.

公开(公告)号：US20210180113A1

公开(公告)日：2021-06-17

申请号：US17068062

申请日：2020-10-12

Applicant: ILLUMINA, INC.

Inventor： Raquel Maria Sanches-Kuiper ,  Vincent Peter Smith ,  Sean Humphray

IPC: C12Q1/6806 ,  C12N15/10

Abstract: Embodiments provided herein relate to methods and compositions for preparing nucleic acid libraries. Some embodiments include preparing libraries from nucleic acids obtained from degraded samples, such as ancient samples and fixed samples.

公开(公告)号：US20180100193A1

公开(公告)日：2018-04-12

申请号：US15836011

申请日：2017-12-08

Applicant: ILLUMINA, INC.

Inventor： Kay Klausing ,  Min-Jui Richard Shen ,  John Moore ,  Vincent Peter Smith ,  Kevin Hall

IPC: C40B20/04 ,  C12N15/10

CPC classification number: C12Q1/6874 ,  C12N15/1003 ,  C12Q1/6832 ,  C12Q1/6869 ,  C40B20/04 ,  C12Q2523/319 ,  C12Q2527/125 ,  C12Q2527/127

Abstract: A method of inhibiting light-induced degradation of nucleic acids includes irradiating a portion of the nucleic acids in the presence of a detection solution comprising a polyphenolic compound. A method of detecting a nucleic acid having a fluorescent tag includes irradiating at least a portion of the nucleic acid with light of a suitable wavelength to induce a fluorescence emission and detecting the fluorescence emission. Optionally, the polyphenolic compound is gallic acid, a lower alkyl ester thereof, or mixtures thereof. A kit includes one or more nucleotides, an enzyme capable of catalyzing incorporation of the nucleotides into a nucleic acid strand and a polyphenolic compound suitable for preparing a detection solution.

公开(公告)号：US20150259737A1

公开(公告)日：2015-09-17

申请号：US14729376

申请日：2015-06-03

Applicant: Illumina, Inc.

Inventor： Kay Klausing ,  Min-Jui Richard Shen ,  John Moore ,  Vincent Peter Smith ,  Kevin Hall

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12N15/1003 ,  C12Q1/6832 ,  C12Q1/6869 ,  C40B20/04 ,  C12Q2523/319 ,  C12Q2527/125 ,  C12Q2527/127

Abstract: A method of inhibiting light-induced degradation of nucleic acids includes irradiating a portion of the nucleic acids in the presence of a detection solution comprising a polyphenolic compound. A method of detecting a nucleic acid having a fluorescent tag includes irradiating at least a portion of the nucleic acid with light of a suitable wavelength to induce a fluorescence emission and detecting the fluorescence emission. Optionally, the polyphenolic compound is gallic acid, a lower alkyl ester thereof, or mixtures thereof. A kit includes one or more nucleotides, an enzyme capable of catalyzing incorporation of the nucleotides into a nucleic acid strand and a polyphenolic compound suitable for preparing a detection solution.

Abstract translation: 抑制光诱导的核酸降解的方法包括在存在包含多酚化合物的检测溶液的情况下照射一部分核酸。 检测具有荧光标签的核酸的方法包括用合适波长的光照射核酸的至少一部分以诱导荧光发射并检测荧光发射。 任选地，多酚化合物是没食子酸，其低级烷基酯或其混合物。 试剂盒包括一个或多个核苷酸，能够催化将核苷酸掺入核酸链的酶和适于制备检测溶液的多酚化合物。

公开(公告)号：US20230272453A1

公开(公告)日：2023-08-31

申请号：US18051836

申请日：2022-11-01

Applicant: ILLUMINA, INC.

Inventor： Raquel Maria Sanches-Kuiper ,  Vincent Peter Smith ,  Sean Humphray

IPC: C12Q1/6806 ,  C12N15/10

CPC classification number: C12Q1/6806 ,  C12N15/1093

Abstract: Embodiments provided herein relate to methods and compositions for preparing nucleic acid libraries. Some embodiments include preparing libraries from nucleic acids obtained from degraded samples, such as ancient samples and fixed samples.

公开(公告)号：US10683532B2

公开(公告)日：2020-06-16

申请号：US16372793

申请日：2019-04-02

Applicant: ILLUMINA, INC.

Inventor： Raquel Maria Sanches-Kuiper ,  Vincent Peter Smith ,  Sean Humphray

IPC: C12Q1/6806 ,  C12N15/10

Abstract: Embodiments provided herein relate to methods and compositions for preparing nucleic acid libraries. Some embodiments include preparing libraries from nucleic acids obtained from degraded samples, such as ancient samples and fixed samples.

公开(公告)号：US10106851B2

公开(公告)日：2018-10-23

申请号：US15836011

申请日：2017-12-08

Applicant: ILLUMINA, INC.

Inventor： Kay Klausing ,  Min-Jui Richard Shen ,  John Moore ,  Vincent Peter Smith ,  Kevin Hall

IPC: C12Q1/68 ,  C12N15/10 ,  C12Q1/6874 ,  C40B20/04 ,  C12Q1/6832 ,  C12Q1/6869

Abstract: A method of inhibiting light-induced degradation of nucleic acids includes irradiating a portion of the nucleic acids in the presence of a detection solution comprising a polyphenolic compound. A method of detecting a nucleic acid having a fluorescent tag includes irradiating at least a portion of the nucleic acid with light of a suitable wavelength to induce a fluorescence emission and detecting the fluorescence emission. Optionally, the polyphenolic compound is gallic acid, a lower alkyl ester thereof, or mixtures thereof. A kit includes one or more nucleotides, an enzyme capable of catalyzing incorporation of the nucleotides into a nucleic acid strand and a polyphenolic compound suitable for preparing a detection solution.