公开(公告)号：US11866753B2

公开(公告)日：2024-01-09

申请号：US17329960

申请日：2021-05-25

Applicant: Inbicon A/S

Inventor： Jan Larsen ,  Niels Nielsen Poulsen ,  Martin Dan Jeppesen ,  Kit Kellebjerg Mogensen

IPC: C12P19/14 ,  D21C1/04 ,  C12P7/10 ,  D21C3/04 ,  C12P19/02 ,  C13K1/02 ,  C13K13/00

CPC classification number: C12P19/14 ,  C12P7/10 ,  C12P19/02 ,  C13K1/02 ,  C13K13/002 ,  D21C1/04 ,  D21C3/04 ,  C12P2201/00 ,  Y02E50/10

Abstract: The invention relates, in general, to methods of processing Lignocellulosic biomass to fermentable sugars and to methods that rely on hydrothermal pretreatment. Xylose monomer yields comparable to those achieved using two-stage pretreatments can be achieved from soft Lignocellulosic biomass feedstocks by pretreasting to very low severity in a single-stage pressurized hydrothermal pretreatment, followed by enzymatic hydrolysis to release xylose retained in the solid state. In some embodiments, pretreated biomass is separated into a solid fraction and a liquid fraction, the solid fraction subjected to enzymatic hydrolysis, and the separated liquid fraction subsequently mixed with the hydrolysed solid fraction.

公开(公告)号：US09920345B2

公开(公告)日：2018-03-20

申请号：US14908913

申请日：2014-02-05

Applicant: Inbicon A/S

Inventor： Jan Larsen ,  Niels Nielsen Poulsen ,  Martin Dan Jeppesen ,  Kit Kellebjerg Mogensen

IPC: C12P19/14 ,  C12P7/10 ,  D21C1/02 ,  D21C1/04 ,  D21C5/00 ,  C12P19/02 ,  C12P5/02

CPC classification number: C12P19/14 ,  C12P5/023 ,  C12P7/10 ,  C12P19/02 ,  C12P2201/00 ,  D21C1/02 ,  D21C1/04 ,  D21C5/005 ,  Y02E50/16 ,  Y02E50/343

Abstract: Methods of processing lignocellulosic biomass to fermentable sugars are provided which rely on hydrothermal pretreatment. Soft lignocellulosic biomass feedstock is pretreated in a single-stage pressurized hydrothermal pretreatment to very low severity. The pre-treated biomass is hydrolysed, typically as a whole slurry, using enzymatic hydrolysis catalysed by an enzyme mixture comprising endoglucanase, exoglucanase, β-glucosidase, endoxylanase, and β-xylosidase activities at activity levels in nkat/g glucan of endoglucanase of at least 1100, exoglucanase of at least 280, β-glucosidase of at least 3000, endoxylanase of at least 1400, and β-xylosidase of at least 75, so as to produce a hydrolysate in which the yield of C5 monomers is at least 55% of the original xylose and arabinose content of the feedstock prior to pretreatment.

公开(公告)号：US20160160253A1

公开(公告)日：2016-06-09

申请号：US14908913

申请日：2014-02-05

Applicant: INBICON A/S

Inventor： Jan Larsen ,  Niels Nielsen Poulsen ,  Martin Dan Jeppesen ,  Kit Kellebjerg Mogensen

IPC: C12P19/14 ,  C12P19/02

CPC classification number: C12P19/14 ,  C12P5/023 ,  C12P7/10 ,  C12P19/02 ,  C12P2201/00 ,  D21C1/02 ,  D21C1/04 ,  D21C5/005 ,  Y02E50/16 ,  Y02E50/343

Abstract: Methods of processing lignocellulosic biomass to fermentable sugars are provided which rely on hydrothermal pretreatment. Soft lignocellulosic biomass feedstock is pretreated in a single-stage pressurized hydrothermal pretreatment to very low severity. The pre-treated biomass is hydrolysed, typically as a whole slurry, using enzymatic hydrolysis catalysed by an enzyme mixture comprising endoglucanase, exoglucanase, β-glucosidase, endoxylanase, and β-xylosidase activities at activity levels in nkat/g glucan of endoglucanase of at least 1100, exoglucanase of at least 280, β-glucosidase of at least 3000, endoxylanase of at least 1400, and β-xylosidase of at least 75, so as to produce a hydrolysate in which the yield of C5 monomers is at least 55% of the original xylose and arabinose content of the feedstock prior to pretreatment.

Abstract translation: 提供了依靠水热预处理将木质纤维素生物质加工成可发酵糖的方法。 软木质纤维素生物质原料在单级加压水热预处理中预处理至非常低的严重性。 使用由含有内切葡聚糖酶，外切葡聚糖酶，葡萄糖苷酶，内切木聚糖酶和β-木糖苷酶活性的酶混合物催化的酶水解，预处理的生物质通常作为整体浆液水解，其活性水平在内切葡聚糖酶的nkat / g葡聚糖 至少为1100，至少280的外切葡聚糖酶，至少为3000的葡萄糖苷酶，至少为1400的内切木聚糖酶，至少为75的木糖苷酶，以及至少75的β-木糖苷酶，以产生其中C5单体的产率为 在预处理之前，原料中原始木糖和阿拉伯糖含量的至少55％。