公开(公告)号：US20150191758A1

公开(公告)日：2015-07-09

申请号：US14418667

申请日：2013-08-01

Applicant: Inbicon A/S

Inventor： Jan Larsen ,  Niels Nielsen Poulsen ,  Martin Dan Jeppesen ,  Kit Kellebjerg Mogensen

IPC: C12P19/14 ,  C13K1/02 ,  C13K13/00 ,  C12P19/02

CPC classification number: C12P19/14 ,  C12P7/10 ,  C12P19/02 ,  C12P2201/00 ,  C13K1/02 ,  C13K13/002 ,  D21C1/04 ,  D21C3/04 ,  Y02E50/16

Abstract: The invention relates, in general, to methods of processing lignocellulosic biomass to fermentable sugars and to methods that rely on hydrothermal pretreatment. Xylose monomer yields comparable to those achieved using two-stage pretreatments can be achieved from soft lignocellulosic biomass feedstocks by pretreating to very low severity in a single-stage pressurized hydrothermal pretreatment, followed by enzymatic hydrolysis to release xylose retained in the solid state. In some embodiments, pretreated biomass is separated into a solid fraction and a liquid fraction, the solid fraction subject to enzymatic hydrolysis, and the separated liquid fraction subsequently mixed with the hydrolysed solid fraction.

Abstract translation: 本发明一般涉及将木质纤维素生物质加工成可发酵糖的方法以及依赖于水热预处理的方法。 可以通过在单级加压水热预处理中预处理至非常低的严重性，然后进行酶水解以释放保持在固体状态的木糖，从而可以从软木质纤维素生物质原料获得与使用两阶段预处理的那些相当的木糖单体产率。 在一些实施方案中，预处理的生物质被分离成固体级分和液体级分，固体级分进行酶水解，并且分离的液体级分随后与水解的固体级分混合。

公开(公告)号：US20240102064A1

公开(公告)日：2024-03-28

申请号：US18523358

申请日：2023-11-29

Applicant: Inbicon A/S

Inventor： Jan Larsen ,  Niels Nielsen Poulsen ,  Martin Dan Jeppesen ,  Kit Kellebjerg Mogensen

IPC: C12P19/14 ,  C12P7/10 ,  C12P19/02 ,  C13K1/02 ,  C13K13/00 ,  D21C1/04 ,  D21C3/04

CPC classification number: C12P19/14 ,  C12P7/10 ,  C12P19/02 ,  C13K1/02 ,  C13K13/002 ,  D21C1/04 ,  D21C3/04 ,  C12P2201/00 ,  Y02E50/10

Abstract: The invention relates, in general, to methods of processing Lignocellulosic biomass to fermentable sugars and to methods that rely on hydrothermal pretreatment. Xylose monomer yields comparable to those achieved using two-stage pretreatments can be achieved from soft Lignocellulosic biomass feedstocks by pretreasting to very low severity in a single-stage pressurized hydrothermal pretreatment, followed by enzymatic hydrolysis to release xylose retained in the solid state. In some embodiments, pretreated biomass is separated into a solid fraction and a liquid fraction, the solid fraction subjected to enzymatic hydrolysis, and the separated liquid fraction subsequently mixed with the hydrolysed solid fraction.

公开(公告)号：US20210348202A1

公开(公告)日：2021-11-11

申请号：US17329960

申请日：2021-05-25

Applicant: Inbicon A/S

Inventor： Jan Larsen ,  Niels Nielsen Poulsen ,  Martin Dan Jeppesen ,  Kit Kellebjerg Mogensen

IPC: C12P19/14 ,  D21C1/04 ,  C12P7/10 ,  D21C3/04 ,  C12P19/02 ,  C13K1/02 ,  C13K13/00

Abstract: The invention relates, in general, to methods of processing Lignocellulosic biomass to fermentable sugars and to methods that rely on hydrothermal pretreatment. Xylose monomer yields comparable to those achieved using two-stage pretreatments can be achieved from soft Lignocellulosic biomass feedstocks by pretreasting to very low severity in a single-stage pressurized hydrothermal pretreatment, followed by enzymatic hydrolysis to release xylose retained in the solid state. In some embodiments, pretreated biomass is separated into a solid fraction and a liquid fraction, the solid fraction subjected to enzymatic hydrolysis, and the separated liquid fraction subsequently mixed with the hydrolysed solid fraction.

公开(公告)号：US20160215448A1

公开(公告)日：2016-07-28

申请号：US14910531

申请日：2014-08-11

Applicant: INBICON A/S

Inventor： Jesper Dohrup ,  Brian Eskesen ,  Kit Kellebjerg Mogensen

IPC: D21C1/10 ,  D21C1/02

CPC classification number: D21C1/10 ,  B01J3/002 ,  B01J3/008 ,  B01J3/02 ,  B01J3/04 ,  B01J2219/00058 ,  B01J2219/00065 ,  B01J2219/00067 ,  B01J2219/00162 ,  B01J2219/00177 ,  B01J2219/0018 ,  B01J2219/00182 ,  B01J2219/00189 ,  C10L5/44 ,  C10L9/086 ,  C12P2201/00 ,  D21C1/02 ,  Y02E50/10 ,  Y02E50/30

Abstract: Provided is a discharge device for discharging pretreated biomass from a pressurized reactor. The device comprises: a vessel having an opening to a high pressure region at the top, and configured to be connected with a pressurized biomass pretreatment device; one or more inlet openings situated along the sides of the vessels through which water or liquid may be added; an orifice or valve at a lower part of said vessel, said orifice or valve being configured to eject pretreated biomass, optionally into a pipeline. The discharge device is characterised in that it comprises mechanical agitation means, said agitation means comprising an agitation element arranged in the interior of said vessel at a lower part of said vessel, and being configured to provide agitation of the content of said vessel, wherein said agitation means being adapted to withstand a pressure in the interior of a said vessel of 10 bar or more. The agitation means provides for a temperature equalization within a specific vertical range of heights of an aqueous slurry present in said vessel, thereby eliminating disadvantages of the prior art devices and methods.

Abstract translation: 提供一种用于从加压反应器中排出预处理的生物质的排放装置。 该装置包括：容器，其具有在顶部处于高压区域的开口，并且构造成与加压的生物质预处理装置连接; 沿着容器侧面设置的一个或多个入口开口，水或液体可以通过该入口开口; 在所述容器的下部的孔或阀，所述孔或阀构造成可任选地排出预处理的生物质到管道中。 排放装置的特征在于它包括机械搅拌装置，所述搅拌装置包括在所述容器的下部设置在所述容器的内部的搅拌元件，并且构造成提供所述容器的内容物的搅拌，其中所述 搅拌装置适于承受10巴或更多的所述容器内部的压力。 搅拌装置提供在所述容器中存在的含水浆料的特定垂直垂直范围内的温度均衡，从而消除现有技术装置和方法的缺点。

公开(公告)号：US11118203B2

公开(公告)日：2021-09-14

申请号：US14418667

申请日：2013-08-01

Applicant: Inbicon A/S

Inventor： Jan Larsen ,  Niels Nielsen Poulsen ,  Martin Dan Jeppesen ,  Kit Kellebjerg Mogensen

IPC: C12P19/14 ,  C12P19/02 ,  C13K1/02 ,  C13K13/00 ,  D21C1/04 ,  C12P7/10 ,  D21C3/04

Abstract: The invention relates, in general, to methods of processing lignocellulosic biomass to fermentable sugars and to methods that rely on hydrothermal pretreatment. Xylose monomer yields comparable to those achieved using two-stage pretreatments can be achieved from soft lignocellulosic biomass feedstocks by pretreating to very low severity in a single-stage pressurized hydrothermal pretreatment, followed by enzymatic hydrolysis to release xylose retained in the solid state. In some embodiments, pretreated biomass is separated into a solid fraction and a liquid fraction, the solid fraction subjected to enzymatic hydrolysis, and the separated liquid fraction subsequently mixed with the hydrolysed solid fraction.

公开(公告)号：US10087578B2

公开(公告)日：2018-10-02

申请号：US14910531

申请日：2014-08-11

Applicant: Inbicon A/S

Inventor： Jesper Dohrup ,  Brian Eskesen ,  Kit Kellebjerg Mogensen

IPC: B01J3/02 ,  D21C1/10 ,  C10L5/44 ,  C10L9/08 ,  B01J3/00 ,  B01J3/04 ,  D21C1/02

Abstract: Provided is a discharge device for discharging pretreated biomass from a pressurized reactor. The device comprises: a vessel having an opening to a high pressure region at the top, and configured to be connected with a pressurized biomass pretreatment device; one or more inlet openings situated along the sides of the vessels through which water or liquid may be added; an orifice or valve at a lower part of said vessel, said orifice or valve being configured to eject pretreated biomass, optionally into a pipeline. The discharge device is characterised in that it comprises mechanical agitation means, said agitation means comprising an agitation element arranged in the interior of said vessel at a lower part of said vessel, and being configured to provide agitation of the content of said vessel, wherein said agitation means being adapted to withstand a pressure in the interior of a said vessel of 10 bar or more. The agitation means provides for a temperature equalization within a specific vertical range of heights of an aqueous slurry present in said vessel, thereby eliminating disadvantages of the prior art devices and methods.

公开(公告)号：US09758843B2

公开(公告)日：2017-09-12

申请号：US14378332

申请日：2013-01-16

Applicant: INBICON A/S

Inventor： Jan Larsen ,  Kit Kellebjerg Mogensen ,  Pia Jørgensen

IPC: C13K13/00 ,  C12P19/02 ,  C12P19/14 ,  C12P7/10

CPC classification number: C13K13/002 ,  C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  C12P2201/00 ,  Y02E50/16

Abstract: The invention relates to a method of processing lignocellulosic biomass comprising: providing lignocellulosic biomass feedstock; pretreating said biomass feedstock by continuous hydrothermal pretreatment in a pressurized reactor; measuring xylan number or lignin number in the output stream of pretreated biomass from the pretreatment reactor; and controlling the pretreatment reactor so as to maintain, in the output stream of pretreated biomass, a pre-determined level of measured xylan number or lignin number. The method allows for continuously controlling the digestibility of a lignocellulosic biomass during the step of pretreatment, and accordingly provides a continuous estimate of the severity of the pretreatment.

公开(公告)号：US20150128933A1

公开(公告)日：2015-05-14

申请号：US14378332

申请日：2013-01-16

Applicant: INBICON A/S

Inventor： Jan Larsen ,  Kit Kellebjerg Mogensen ,  Pia Jørgensen

IPC: C13K13/00

CPC classification number: C13K13/002 ,  C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  C12P2201/00 ,  Y02E50/16

Abstract: The invention relates to a method of processing lignocellulosic biomass comprising: providing lignocellulosic biomass feedstock; pretreating said biomass feedstock by continuous hydrothermal pretreatment in a pressurized reactor; measuring xylan number or lignin number in the output stream of pretreated biomass from the pretreatment reactor; and controlling the pretreatment reactor so as to maintain, in the output stream of pretreated biomass, a pre-determined level of measured xylan number or lignin number. The method allows for continuously controlling the digestibility of a lignocellulosic biomass during the step of pretreatment, and accordingly provides a continuous estimate of the severity of the pretreatment.

Abstract translation: 本发明涉及一种处理木质纤维素生物质的方法，包括：提供木质纤维素生物质原料; 在加压反应器中通过连续水热预处理预处理所述生物质原料; 测量来自预处理反应器的预处理生物质的输出流中的木聚糖数或木质素数; 并控制预处理反应器，以便在预处理的生物质的输出流中维持测定的木聚糖数量或木质素数量的预定水平。 该方法允许在预处理步骤期间连续控制木质纤维素生物质的消化率，因此提供对预处理的严重性的连续估计。

公开(公告)号：US11866753B2

公开(公告)日：2024-01-09

申请号：US17329960

申请日：2021-05-25

Applicant: Inbicon A/S

Inventor： Jan Larsen ,  Niels Nielsen Poulsen ,  Martin Dan Jeppesen ,  Kit Kellebjerg Mogensen

IPC: C12P19/14 ,  D21C1/04 ,  C12P7/10 ,  D21C3/04 ,  C12P19/02 ,  C13K1/02 ,  C13K13/00

CPC classification number: C12P19/14 ,  C12P7/10 ,  C12P19/02 ,  C13K1/02 ,  C13K13/002 ,  D21C1/04 ,  D21C3/04 ,  C12P2201/00 ,  Y02E50/10

Abstract: The invention relates, in general, to methods of processing Lignocellulosic biomass to fermentable sugars and to methods that rely on hydrothermal pretreatment. Xylose monomer yields comparable to those achieved using two-stage pretreatments can be achieved from soft Lignocellulosic biomass feedstocks by pretreasting to very low severity in a single-stage pressurized hydrothermal pretreatment, followed by enzymatic hydrolysis to release xylose retained in the solid state. In some embodiments, pretreated biomass is separated into a solid fraction and a liquid fraction, the solid fraction subjected to enzymatic hydrolysis, and the separated liquid fraction subsequently mixed with the hydrolysed solid fraction.

公开(公告)号：US09920345B2

公开(公告)日：2018-03-20

申请号：US14908913

申请日：2014-02-05

Applicant: Inbicon A/S

Inventor： Jan Larsen ,  Niels Nielsen Poulsen ,  Martin Dan Jeppesen ,  Kit Kellebjerg Mogensen

IPC: C12P19/14 ,  C12P7/10 ,  D21C1/02 ,  D21C1/04 ,  D21C5/00 ,  C12P19/02 ,  C12P5/02

CPC classification number: C12P19/14 ,  C12P5/023 ,  C12P7/10 ,  C12P19/02 ,  C12P2201/00 ,  D21C1/02 ,  D21C1/04 ,  D21C5/005 ,  Y02E50/16 ,  Y02E50/343

Abstract: Methods of processing lignocellulosic biomass to fermentable sugars are provided which rely on hydrothermal pretreatment. Soft lignocellulosic biomass feedstock is pretreated in a single-stage pressurized hydrothermal pretreatment to very low severity. The pre-treated biomass is hydrolysed, typically as a whole slurry, using enzymatic hydrolysis catalysed by an enzyme mixture comprising endoglucanase, exoglucanase, β-glucosidase, endoxylanase, and β-xylosidase activities at activity levels in nkat/g glucan of endoglucanase of at least 1100, exoglucanase of at least 280, β-glucosidase of at least 3000, endoxylanase of at least 1400, and β-xylosidase of at least 75, so as to produce a hydrolysate in which the yield of C5 monomers is at least 55% of the original xylose and arabinose content of the feedstock prior to pretreatment.