公开(公告)号：US20150191758A1

公开(公告)日：2015-07-09

申请号：US14418667

申请日：2013-08-01

Applicant: Inbicon A/S

Inventor： Jan Larsen ,  Niels Nielsen Poulsen ,  Martin Dan Jeppesen ,  Kit Kellebjerg Mogensen

IPC: C12P19/14 ,  C13K1/02 ,  C13K13/00 ,  C12P19/02

CPC classification number: C12P19/14 ,  C12P7/10 ,  C12P19/02 ,  C12P2201/00 ,  C13K1/02 ,  C13K13/002 ,  D21C1/04 ,  D21C3/04 ,  Y02E50/16

Abstract: The invention relates, in general, to methods of processing lignocellulosic biomass to fermentable sugars and to methods that rely on hydrothermal pretreatment. Xylose monomer yields comparable to those achieved using two-stage pretreatments can be achieved from soft lignocellulosic biomass feedstocks by pretreating to very low severity in a single-stage pressurized hydrothermal pretreatment, followed by enzymatic hydrolysis to release xylose retained in the solid state. In some embodiments, pretreated biomass is separated into a solid fraction and a liquid fraction, the solid fraction subject to enzymatic hydrolysis, and the separated liquid fraction subsequently mixed with the hydrolysed solid fraction.

Abstract translation: 本发明一般涉及将木质纤维素生物质加工成可发酵糖的方法以及依赖于水热预处理的方法。 可以通过在单级加压水热预处理中预处理至非常低的严重性，然后进行酶水解以释放保持在固体状态的木糖，从而可以从软木质纤维素生物质原料获得与使用两阶段预处理的那些相当的木糖单体产率。 在一些实施方案中，预处理的生物质被分离成固体级分和液体级分，固体级分进行酶水解，并且分离的液体级分随后与水解的固体级分混合。

公开(公告)号：US11118203B2

公开(公告)日：2021-09-14

申请号：US14418667

申请日：2013-08-01

Applicant: Inbicon A/S

Inventor： Jan Larsen ,  Niels Nielsen Poulsen ,  Martin Dan Jeppesen ,  Kit Kellebjerg Mogensen

IPC: C12P19/14 ,  C12P19/02 ,  C13K1/02 ,  C13K13/00 ,  D21C1/04 ,  C12P7/10 ,  D21C3/04

Abstract: The invention relates, in general, to methods of processing lignocellulosic biomass to fermentable sugars and to methods that rely on hydrothermal pretreatment. Xylose monomer yields comparable to those achieved using two-stage pretreatments can be achieved from soft lignocellulosic biomass feedstocks by pretreating to very low severity in a single-stage pressurized hydrothermal pretreatment, followed by enzymatic hydrolysis to release xylose retained in the solid state. In some embodiments, pretreated biomass is separated into a solid fraction and a liquid fraction, the solid fraction subjected to enzymatic hydrolysis, and the separated liquid fraction subsequently mixed with the hydrolysed solid fraction.

公开(公告)号：US20190271017A1

公开(公告)日：2019-09-05

申请号：US16347091

申请日：2017-11-06

Applicant: Inbicon A/S

Inventor： Martin Dan Jeppesen ,  Jan Larsen ,  Kit Haubjerg Mogensen ,  Anna Frederike Gossmann ,  Anna Granly Hansen ,  Laila Thirup ,  Lars Villadsgaard Toft ,  Hanne Risbjerg Sørensen

IPC: C12P19/02 ,  C12P19/14 ,  C13K1/02 ,  C12P7/10 ,  C07G1/00 ,  C08L95/00

Abstract: A method for providing a C5/C6 product from a lignocellulosic material is disclosed, said method comprising the steps: (i) pretreatment of the lignocellulosic material; (ii) solid/liquid separation of the pretreated lignocellulosic material from step (a) into a first solid fraction and a first liquid fraction; (iii) enzymatic fiber hydrolysis of said first solid fraction from step (b) by use of an enzyme composition capable of degrading lignocellulosic material, thereby providing a C5/C6 fiber slurry comprising C5 and/or C6 sugars; (iv) solid/liquid separation of the C5/C6 fiber slurry from step (c) into a second solid fraction and a second liquid fraction; and optionally (v) combining said first liquid fraction and said second liquid fraction for enzymatic mixed sugar hydrolysis (MSH), whereby a MSH C5/C6 product is provided.

公开(公告)号：US09758843B2

公开(公告)日：2017-09-12

申请号：US14378332

申请日：2013-01-16

Applicant: INBICON A/S

Inventor： Jan Larsen ,  Kit Kellebjerg Mogensen ,  Pia Jørgensen

IPC: C13K13/00 ,  C12P19/02 ,  C12P19/14 ,  C12P7/10

CPC classification number: C13K13/002 ,  C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  C12P2201/00 ,  Y02E50/16

Abstract: The invention relates to a method of processing lignocellulosic biomass comprising: providing lignocellulosic biomass feedstock; pretreating said biomass feedstock by continuous hydrothermal pretreatment in a pressurized reactor; measuring xylan number or lignin number in the output stream of pretreated biomass from the pretreatment reactor; and controlling the pretreatment reactor so as to maintain, in the output stream of pretreated biomass, a pre-determined level of measured xylan number or lignin number. The method allows for continuously controlling the digestibility of a lignocellulosic biomass during the step of pretreatment, and accordingly provides a continuous estimate of the severity of the pretreatment.

公开(公告)号：US20150299734A1

公开(公告)日：2015-10-22

申请号：US14253890

申请日：2014-04-16

Applicant: Inbicon A/S

Inventor： Jan Larsen

IPC: C12P7/06

CPC classification number: C12P7/06 ,  A01N65/44 ,  C12N1/16 ,  C12N1/22 ,  C12P7/10 ,  Y02E50/16 ,  Y02E50/17

Abstract: A range of concentrations exists in which fermentation inhibitors derived from pretreatment of lignocellulosic feed stocks inhibit growth of lactic acid bacteria without affecting fermentive yeast. By optimizing levels of fermentation inhibitors to fall within this range, yeast fermentations of lignocellulosic biomass can be conducted under non-sterile conditions with ethanol yields comparable to those achieved under sterile conditions. Optimised inhibitor levels can be achieved by controlling the water/biomass ratio of a lignocellulosic biomass during and after pretreatment, for example by washing the fiber fraction of a previously pretreated lignocellulosic biomass with a pre-defined amount of fresh water or recycled process solutions. Crude extracts of liquid fraction or process solutions from pretreatment of lignocellulosic biomass can also provide an effective anti-baterial treatment for first generation starch fermentations.

Abstract translation: 存在一定范围的浓度，其中衍生自木质纤维素原料的预处理的发酵抑制剂抑制乳酸菌的生长而不影响发酵酵母。 通过优化发酵抑制剂的含量落在此范围内，木质纤维素生物质的酵母发酵可以在非无菌条件下进行，乙醇产量与在无菌条件下达到的相似。 优化的抑制剂水平可以通过在预处理期间和之后控制木质纤维素生物质的水/生物量比来实现，例如通过用预定量的淡水或再循环的处理溶液洗涤先前预处理的木质纤维素生物质的纤维部分。 来自木质纤维素生物质预处理的液体馏分或处理溶液的粗提取物也可为第一代淀粉发酵提供有效的抗物质处理。

公开(公告)号：US20150128933A1

公开(公告)日：2015-05-14

申请号：US14378332

申请日：2013-01-16

Applicant: INBICON A/S

Inventor： Jan Larsen ,  Kit Kellebjerg Mogensen ,  Pia Jørgensen

IPC: C13K13/00

CPC classification number: C13K13/002 ,  C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  C12P2201/00 ,  Y02E50/16

Abstract: The invention relates to a method of processing lignocellulosic biomass comprising: providing lignocellulosic biomass feedstock; pretreating said biomass feedstock by continuous hydrothermal pretreatment in a pressurized reactor; measuring xylan number or lignin number in the output stream of pretreated biomass from the pretreatment reactor; and controlling the pretreatment reactor so as to maintain, in the output stream of pretreated biomass, a pre-determined level of measured xylan number or lignin number. The method allows for continuously controlling the digestibility of a lignocellulosic biomass during the step of pretreatment, and accordingly provides a continuous estimate of the severity of the pretreatment.

Abstract translation: 本发明涉及一种处理木质纤维素生物质的方法，包括：提供木质纤维素生物质原料; 在加压反应器中通过连续水热预处理预处理所述生物质原料; 测量来自预处理反应器的预处理生物质的输出流中的木聚糖数或木质素数; 并控制预处理反应器，以便在预处理的生物质的输出流中维持测定的木聚糖数量或木质素数量的预定水平。 该方法允许在预处理步骤期间连续控制木质纤维素生物质的消化率，因此提供对预处理的严重性的连续估计。

公开(公告)号：US12188070B2

公开(公告)日：2025-01-07

申请号：US16347091

申请日：2017-11-06

Applicant: Inbicon A/S

Inventor： Martin Dan Jeppesen ,  Jan Larsen ,  Kit Haubjerg Mogensen ,  Anna Frederike Gossmann ,  Anna Granly Hansen ,  Laila Thirup ,  Lars Villadsgaard Toft ,  Hanne Risbjerg Sørensen

IPC: C12P19/02 ,  C07G1/00 ,  C08L95/00 ,  C12P7/10 ,  C12P19/14 ,  C13K1/02 ,  C13K13/00

Abstract: A method for providing a C5/C6 product from a lignocellulosic material is disclosed, said method comprising the steps: (i) pretreatment of the lignocellulosic material; (ii) solid/liquid separation of the pretreated lignocellulosic material from step (a) into a first solid fraction and a first liquid fraction; (iii) enzymatic fiber hydrolysis of said first solid fraction from step (b) by use of an enzyme composition capable of degrading lignocellulosic material, thereby providing a C5/C6 fiber slurry comprising C5 and/or C6 sugars; (iv) solid/liquid separation of the C5/C6 fiber slurry from step (c) into a second solid fraction and a second liquid fraction; and optionally (v) combining said first liquid fraction and said second liquid fraction for enzymatic mixed sugar hydrolysis (MSH), whereby a MSH C5/C6 product is provided.

公开(公告)号：US20240102064A1

公开(公告)日：2024-03-28

申请号：US18523358

申请日：2023-11-29

Applicant: Inbicon A/S

Inventor： Jan Larsen ,  Niels Nielsen Poulsen ,  Martin Dan Jeppesen ,  Kit Kellebjerg Mogensen

IPC: C12P19/14 ,  C12P7/10 ,  C12P19/02 ,  C13K1/02 ,  C13K13/00 ,  D21C1/04 ,  D21C3/04

CPC classification number: C12P19/14 ,  C12P7/10 ,  C12P19/02 ,  C13K1/02 ,  C13K13/002 ,  D21C1/04 ,  D21C3/04 ,  C12P2201/00 ,  Y02E50/10

Abstract: The invention relates, in general, to methods of processing Lignocellulosic biomass to fermentable sugars and to methods that rely on hydrothermal pretreatment. Xylose monomer yields comparable to those achieved using two-stage pretreatments can be achieved from soft Lignocellulosic biomass feedstocks by pretreasting to very low severity in a single-stage pressurized hydrothermal pretreatment, followed by enzymatic hydrolysis to release xylose retained in the solid state. In some embodiments, pretreated biomass is separated into a solid fraction and a liquid fraction, the solid fraction subjected to enzymatic hydrolysis, and the separated liquid fraction subsequently mixed with the hydrolysed solid fraction.

公开(公告)号：US20210348202A1

公开(公告)日：2021-11-11

申请号：US17329960

申请日：2021-05-25

Applicant: Inbicon A/S

Inventor： Jan Larsen ,  Niels Nielsen Poulsen ,  Martin Dan Jeppesen ,  Kit Kellebjerg Mogensen

IPC: C12P19/14 ,  D21C1/04 ,  C12P7/10 ,  D21C3/04 ,  C12P19/02 ,  C13K1/02 ,  C13K13/00

Abstract: The invention relates, in general, to methods of processing Lignocellulosic biomass to fermentable sugars and to methods that rely on hydrothermal pretreatment. Xylose monomer yields comparable to those achieved using two-stage pretreatments can be achieved from soft Lignocellulosic biomass feedstocks by pretreasting to very low severity in a single-stage pressurized hydrothermal pretreatment, followed by enzymatic hydrolysis to release xylose retained in the solid state. In some embodiments, pretreated biomass is separated into a solid fraction and a liquid fraction, the solid fraction subjected to enzymatic hydrolysis, and the separated liquid fraction subsequently mixed with the hydrolysed solid fraction.

公开(公告)号：US20170009261A9

公开(公告)日：2017-01-12

申请号：US14253890

申请日：2014-04-16

Applicant: Inbicon A/S

Inventor： Jan Larsen

IPC: C12P7/06

CPC classification number: C12P7/06 ,  A01N65/44 ,  C12N1/16 ,  C12N1/22 ,  C12P7/10 ,  Y02E50/16 ,  Y02E50/17

Abstract: A range of concentrations exists in which fermentation inhibitors derived from pretreatment of lignocellulosic feed stocks inhibit growth of lactic acid bacteria without affecting fermentive yeast. By optimizing levels of fermentation inhibitors to fall within this range, yeast fermentations of lignocellulosic biomass can be conducted under non-sterile conditions with ethanol yields comparable to those achieved under sterile conditions. Optimised inhibitor levels can be achieved by controlling the water/biomass ratio of a lignocellulosic biomass during and after pretreatment, for example by washing the fiber fraction of a previously pretreated lignocellulosic biomass with a pre-defined amount of fresh water or recycled process solutions. Crude extracts of liquid fraction or process solutions from pretreatment of lignocellulosic biomass can also provide an effective anti-baterial treatment for first generation starch fermentations.

Abstract translation: 存在一定范围的浓度，其中衍生自木质纤维素原料的预处理的发酵抑制剂抑制乳酸菌的生长而不影响发酵酵母。 通过优化发酵抑制剂的含量落在此范围内，木质纤维素生物质的酵母发酵可以在非无菌条件下进行，乙醇产量与在无菌条件下达到的相似。 优化的抑制剂水平可以通过在预处理期间和之后控制木质纤维素生物质的水/生物量比来实现，例如通过用预定量的淡水或再循环的处理溶液洗涤先前预处理的木质纤维素生物质的纤维部分。 来自木质纤维素生物质预处理的液体馏分或处理溶液的粗提取物也可为第一代淀粉发酵提供有效的抗物质处理。