公开(公告)号：US10294473B2

公开(公告)日：2019-05-21

申请号：US15948793

申请日：2018-04-09

Applicant: The Regents of the University of Colorado, a body corporate ,  Inscripta, Inc.

Inventor： Ryan T. Gill ,  Andrew Garst ,  Tanya Elizabeth Warnecke Lipscomb ,  Marcelo Colika Bassalo ,  Ramsey Ibrahim Zeitoun

IPC: C12N15/10 ,  C12N15/11

Abstract: Provided herein are methods and composition for trackable genetic variant libraries. Further provided herein are methods and compositions for recursive engineering. Further provided herein are methods and compositions for multiplex engineering. Further provided herein are methods and compositions for enriching for editing and trackable engineered sequences and cells using nucleic acid-guided nucleases.

公开(公告)号：US10011849B1

公开(公告)日：2018-07-03

申请号：US15631989

申请日：2017-06-23

Applicant: Inscripta, Inc.

Inventor： Ryan T. Gill ,  Andrew Garst ,  Tanya Elizabeth Warnecke Lipscomb

IPC: C12N15/90 ,  C12N15/11 ,  C12N15/10 ,  C12N15/82 ,  C12N15/85 ,  C12N9/22 ,  C12N5/14 ,  C12N5/16 ,  C12N5/22 ,  C12N15/09 ,  C12N15/52 ,  C12N15/63 ,  C12N15/79 ,  C12N15/70 ,  C12N15/81

CPC classification number: C12N15/902 ,  C12N9/22 ,  C12N15/11 ,  C12N15/111 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85 ,  C12N15/905 ,  C12N15/907 ,  C12N2310/20 ,  C12N2800/22

Abstract: Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods.

公开(公告)号：US12180502B2

公开(公告)日：2024-12-31

申请号：US17829316

申请日：2022-05-31

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Ryan T. Gill ,  Tanya Elizabeth Warnecke Lipscomb

IPC: C12N15/90 ,  C07K14/195 ,  C12N9/22 ,  C12N15/10 ,  C12N15/11 ,  C12N15/113 ,  C12N15/63 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85

Abstract: Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods.

公开(公告)号：US20220389417A1

公开(公告)日：2022-12-08

申请号：US17829316

申请日：2022-05-31

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Ryan T. Gill ,  Tanya Elizabeth Warnecke Lipscomb

IPC: C12N15/11 ,  C12N15/90 ,  C12N9/22

Abstract: Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods.

公开(公告)号：US11408012B2

公开(公告)日：2022-08-09

申请号：US17588256

申请日：2022-01-29

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Ryan T. Gill ,  Tanya Elizabeth Warnecke Lipscomb

IPC: C12N15/90 ,  C12N9/22 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85 ,  C12N15/11 ,  C12N15/10 ,  C12N15/63 ,  C12N15/113

Abstract: Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods.

公开(公告)号：US20220162646A1

公开(公告)日：2022-05-26

申请号：US17588256

申请日：2022-01-29

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Ryan T. Gill ,  Tanya Elizabeth Warnecke Lipscomb

IPC: C12N15/90 ,  C12N9/22 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85 ,  C12N15/11 ,  C12N15/10 ,  C12N15/63 ,  C12N15/113

Abstract: Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods.

公开(公告)号：US11220697B2

公开(公告)日：2022-01-11

申请号：US17387860

申请日：2021-07-28

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Ryan T. Gill ,  Tanya Elizabeth Warnecke Lipscomb

IPC: C12N15/90 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85 ,  C12N9/22 ,  C12N15/11

Abstract: Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods.

公开(公告)号：US10017760B2

公开(公告)日：2018-07-10

申请号：US15632222

申请日：2017-06-23

Applicant: The Regents of the University of Colorado, a body corporate ,  Inscripta, Inc.

Inventor： Ryan T. Gill ,  Andrew Garst ,  Tanya Elizabeth Warnecke Lipscomb ,  Marcelo Colika Bassalo ,  Ramsey Ibrahim Zeitoun

IPC: C12N15/09 ,  C12N15/10 ,  C12N15/11 ,  C12N15/63 ,  C12N15/64 ,  C12N15/70 ,  C12N15/74 ,  C12N15/79 ,  C12N15/81 ,  C12N15/82 ,  C12N15/85

CPC classification number: C12N15/1065 ,  C12N15/102 ,  C12N15/1079 ,  C12N15/1082 ,  C12N15/11 ,  C12N2310/20 ,  C12Q2563/179

Abstract: Provided herein are methods and composition for trackable genetic variant libraries. Further provided herein are methods and compositions for recursive engineering. Further provided herein are methods and compositions for multiplex engineering. Further provided herein are methods and compositions for enriching for editing and trackable engineered sequences and cells using nucleic acid-guided nucleases.

公开(公告)号：US20250066818A1

公开(公告)日：2025-02-27

申请号：US18945973

申请日：2024-11-13

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Ryan T. Gill ,  Tanya Elizabeth Warnecke Lipscomb

IPC: C12N15/90 ,  C12N9/22 ,  C12N15/11 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85

Abstract: Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods.

公开(公告)号：US12195749B2

公开(公告)日：2025-01-14

申请号：US17692069

申请日：2022-03-10

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Ryan T. Gill ,  Tanya Elizabeth Warnecke Lipscomb

IPC: C12N15/90 ,  C12N9/22 ,  C12N15/11 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85

Abstract: Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods.