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    Chemical inducible promoter used to obtain transgenic plants with a silent marker and organisms and cells and methods of using same for screening for mutations
    11.
    发明授权
    Chemical inducible promoter used to obtain transgenic plants with a silent marker and organisms and cells and methods of using same for screening for mutations 失效
    用于获得具有沉默标记和生物体和细胞的转基因植物的化学诱导型启动子以及使用它们筛选突变的方法

    公开(公告)号：US07230157B1

    公开(公告)日：2007-06-12

    申请号：US10129849

    申请日：2000-11-13

    申请人： Jianru Zuo ,  Nam-Hai Chua

    发明人： Jianru Zuo ,  Nam-Hai Chua

    IPC分类号： C12N15/00 ,  C12N15/82 ,  A01H5/00

    CPC分类号： C12N15/8274 ,  C12N15/8209 ,  C12N15/8237 ,  C12N15/8238 ,  C12N15/8242 ,  C12N15/8295

    摘要： Disclosed is a chemically inducible promoter for transforming plants or plant cells with genes which are regulatable by adding the plants or cells to a medium containing an inducer or by removing them from such medium. The promoter is inducible by a glucocorticoid, estrogen or inducer not endogenous to plants. Such promoters may be used with any plant genes that can promote shoot regeneration and development to induce shoot formation in the presence of a glucocorticoid, estrogen or inducer. The promoter may be used with antibiotic or herbicide resistance genes or other genes which are regulatable by the presence or absence of a given inducer. Also presented are organisms or cells comprising a gene wherein the natural promoter of the gene is disrupted and the gene is placed under the control of a transgenic inducible promoter. These organisms and cells and their progeny are useful for screening for conditional gain of function and loss of function mutations.

    摘要翻译： 公开了一种化学诱导型启动子，用于通过将植物或细胞添加到含有诱导物的培养基中或通过从这种培养基中除去可调节基因来转化植物或植物细胞。 启动子可通过糖皮质激素，雌激素或对植物无内源性的诱导物诱导。 这样的启动子可以与任何植物基因一起使用，所述植物基因可以在糖皮质激素，雌激素或诱导物的存在下促进芽再生和发育以诱导芽形成。 启动子可以与抗生素或除草剂抗性基因或其它可通过给定诱导物的存在或不存在而调节的基因一起使用。 还提供了包含基因的生物体或细胞，其中基因的天然启动子被破坏并且将基因置于转基因诱导型启动子的控制下。 这些生物和细胞及其后代可用于筛选功能条件增加和功能突变丧失。

    High frequency plant transformation and/or regeneration
    14.
    发明申请
    High frequency plant transformation and/or regeneration 审中-公开
    高频植物转化和/或再生

    公开(公告)号：US20050060777A1

    公开(公告)日：2005-03-17

    申请号：US10659710

    申请日：2003-09-11

    申请人： Luis Lopez-Molina ,  Qi-Wen Niu ,  Nam-Hai Chua

    发明人： Luis Lopez-Molina ,  Qi-Wen Niu ,  Nam-Hai Chua

    IPC分类号： A01H4/00 ,  C12N15/82 ,  A01H1/00

    CPC分类号： C12N15/8205 ,  A01H4/008

    摘要： The present invention is directed to methods for improving Agrobacterium-mediated plant transformation and regeneration of transformed tissue. More specifically, the present invention provides methods for enhancing the frequency of plant transformation by Agrobacterium in which ABI5, ABI5 ortholog protein or ABI5 homolog protein level or activity in transformed plant tissue is suppressed. Any mechanism which suppresses levels or activity of ABI5, ABI5 ortholog protein or ABI homolog protein in plants can be used to enhance the frequency of plant transformation by Agrobacterium. The present invention is further directed to plants, nucleic acids and vectors for use in the methods of invention.

    摘要翻译： 本发明涉及改进农杆菌介导的植物转化和转化组织再生的方法。 更具体地，本发明提供了通过农杆菌增强植物转化频率的方法，其中ABI5，ABI5直向同源蛋白或ABI5同源蛋白水平或转化植物组织中的活性被抑制。 抑制植物中ABI5，ABI5直向同源蛋白或ABI同源蛋白的水平或活性的任何机制可用于增强农杆菌植物转化的频率。 本发明还涉及用于本发明方法的植物，核酸和载体。

    Promotion of somatic embryogenesis in plants by PGA37 gene expression
    15.
    发明申请
    Promotion of somatic embryogenesis in plants by PGA37 gene expression 有权
    通过PGA37基因表达促进植物体细胞胚发生

    公开(公告)号：US20050262595A1

    公开(公告)日：2005-11-24

    申请号：US10850465

    申请日：2004-05-21

    申请人： Qi-Wen Niu ,  Nam-Hai Chua

    发明人： Qi-Wen Niu ,  Nam-Hai Chua

    IPC分类号： A01H1/00 ,  A01H1/08 ,  A01H4/00 ,  A01H5/00 ,  C07K14/415 ,  C12N15/82

    CPC分类号： C12N15/8261 ,  A01H1/08 ,  A01H4/005 ,  A01H4/008 ,  C07K14/415 ,  C12N15/821 ,  C12N15/8287 ,  Y02A40/146

    摘要： The present invention relates to methods for promoting somatic embryogenesis from a plant cell, tissue, organ, callus or cell culture, by overexpressing a PGA37 gene in the tissue or organ. In one embodiment, such overexpression can be used as a silent selectable marker for transgenic plants. In another embodiment, such expression can be used to confer apomixis to a plant. In another embodiment, such overexpression can be used to create haploid plants, which can be used to produce dihaploid plants.

    摘要翻译： 本发明涉及通过过表达组织或器官中的PGA37基因来促进植物细胞，组织，器官，愈伤组织或细胞培养物中体细胞胚发生的方法。 在一个实施方案中，这种过表达可以用作转基因植物的沉默选择标记。 在另一个实施方案中，这种表达可以用于赋予植物无义变异体。 在另一个实施方案中，这种过表达可用于产生单倍体植物，其可用于产生双倍体植物。

    Inducible site-specific recombination for the activation and removal of transgenes in transgenic plants
    16.
    发明授权
    Inducible site-specific recombination for the activation and removal of transgenes in transgenic plants 有权
    诱导位点特异性重组用于转基因植物中转基因的活化和去除

    公开(公告)号：US06723896B1

    公开(公告)日：2004-04-20

    申请号：US09439534

    申请日：1999-11-12

    申请人： Simon Geir Moller ,  Jianru Zuo ,  Nam-Hai Chua

    发明人： Simon Geir Moller ,  Jianru Zuo ,  Nam-Hai Chua

    IPC分类号： C12N1582

    CPC分类号： C12N15/8237 ,  C12N15/8238

    摘要： Disclosed is an inducible promoter system in conjunction with a site-specific recombination system which allows (i) specific activation of transgenes at specific times or (ii) excision and removal of transgenes (e.g., antibiotic resistance markers) from transgenic plants. These “suicide” gene cassettes, including the recombination system itself, can be evicted from the plant genome once their function has been exerted. The system is based on the ability to temporally and spatially induce the expression of CRE recombinase which then binds to directly repeated lox sites flanking the transgene in question leading to the precise excision of the gene cassette. Also disclosed is a method to activate an inverted, and therefore silent, transgene by placing two lox sites in opposite orientations flanking the transgene. This results in inversion of the intervening DNA fragment in the presence of CRE recombinase. This activation can be timed by placing the CRE recombinase under the control of an inducible promoter.

    摘要翻译： 公开了与位点特异性重组系统结合的诱导型启动子系统，其允许（i）在特定时间特异性转化转基因，或（ii）从转基因植物切除和除去转基因（例如抗生素抗性标记）。 这些“自杀”基因盒，包括重组系统本身，一旦发挥作用就可以从植物基因组中被驱逐出去。 该系统基于时间和空间诱导CRE重组酶表达的能力，其然后结合直接重复的涉及转基因侧翼的lox位点，从而导致基因盒的精确切除。 还公开了通过将两个lox位点放置在转基因侧翼的相反方向上来激活倒置的，因此沉默的转基因的方法。 这导致在CRE重组酶存在下插入的DNA片段的倒置。 可以通过将CRE重组酶置于诱导型启动子的控制下来定时进行该激活。

    SELECTION METHOD
    18.
    发明申请
    SELECTION METHOD 审中-公开
    选择方法

    公开(公告)号：US20100186125A1

    公开(公告)日：2010-07-22

    申请号：US12448263

    申请日：2007-12-14

    申请人： Simon Moller ,  Nam-Hai Chua

    发明人： Simon Moller ,  Nam-Hai Chua

    IPC分类号： A01H5/00 ,  C12N15/82 ,  C12N5/10

    CPC分类号： C12N15/8214

    摘要： The invention relates to a method for producing a transformed plant cell. More particularly, the method involves the transformation of a plant cell with a Transformation Cassette which is targeted to plant plastids and which comprises a selection gene, for example isopentenyl transferase (IPT), and a transgene. After selection for transformed plastids, expression of a recombinase is induced in the plant cell, which leads to the excision of the selection gene from the plastid and the expression of the transgene in the plastid. The invention also provides cells and plants comprising the Transformation Cassette.

    摘要翻译： 本发明涉及一种生产转化植物细胞的方法。 更具体地，该方法涉及用靶向植物质体的转化盒转化植物细胞，其包含选择基因，例如异戊烯基转移酶（IPT）和转基因。 在选择转化质体后，在植物细胞中诱导重组酶的表达，这导致选择基因从质体中切除和转化子在质体中的表达。 本发明还提供包含转化盒的细胞和植物。

    ESR2 - a plant gene that can promote shoot regeneration
    19.
    发明授权
    ESR2 - a plant gene that can promote shoot regeneration 失效
    ESR2 - 可以促进苗再生的植物基因

    公开(公告)号：US06441276B1

    公开(公告)日：2002-08-27

    申请号：US09964850

    申请日：2001-09-28

    申请人： Yoshihisa Ikeda ,  Nam-Hai Chua

    发明人： Yoshihisa Ikeda ,  Nam-Hai Chua

    IPC分类号： C12N1587

    CPC分类号： C12N15/821 ,  C07K14/415 ,  C12N15/8295

    摘要： A plant gene, Esr2, has been found which when overexpressed in plant cells results in cells which have cytokinin-independent cell growth. This feature allows the encoded protein ESR2 to be used as a selectable marker of transformed cells by growing the transformed cells on cytokinin-free media. It has also been found that overexpression of ESR2 in cells grown in the presence of cytokinins results in a higher regeneration of plants. This feature allows the gene to be used to obtain greater regeneration of plant cells.

    摘要翻译： 已经发现植物基因Esr2在植物细胞中过表达时产生具有细胞分裂素依赖性细胞生长的细胞。 该特征允许编码的蛋白质ESR2通过在不含细胞分裂素的培养基上生长转化的细胞而用作转化细胞的选择标记。 还已经发现，在细胞分裂素存在下生长的细胞中ESR2的过表达导致植物更高的再生。 该特征允许该基因用于获得更大的植物细胞再生。

    Alteration of plant morphology by control of profilin expression
    20.
    发明授权
    Alteration of plant morphology by control of profilin expression 失效
    通过控制profilin表达改变植物形态

    公开(公告)号：US06344601B1

    公开(公告)日：2002-02-05

    申请号：US09061897

    申请日：1998-04-17

    申请人： Nam-Hai Chua ,  Srinivasan Ramachandran ,  Hans Erik Molager Christensen

    发明人： Nam-Hai Chua ,  Srinivasan Ramachandran ,  Hans Erik Molager Christensen

    IPC分类号： C12N1582

    CPC分类号： C12N15/827 ,  C12N15/8261 ,  Y02A40/146

    摘要： Plant growth habit is altered by causing either under-expression or over-expression of profilin in a plant cell. Under-expression of profilin can be achieved by transforming a plant or plant cell with a gene expressing an antisense mRNA complementary to the mRNA transcribed by the coding sequence of a profilin gene and expressing the gene in the plant or plant cell such that the antisense mRNA inhibits the production of the profilin in the plant or plant cell. Under-expression of profilins in plants can lead to such alterations in growth habit as a dwarf phenotype, a reduced root and root hair system, and delay in the onset of flowering. Over-expression of profilin can be achieved by transforming a plant or plant cell with a gene capable of expressing a profilin in the plant or plant cell and causing the transformed gene to be expressed in the plant or plant cell. Over-expression of profilin in a plant can lead to such alterations in growth habit as a tall phenotype, an expansion of the root and root hair system, expansion of leaf surface area and accelerating the onset of flowering.

    摘要翻译： 通过在植物细胞中引起profilin的低表达或过度表达来改变植物生长习性。 profilin的低表达可以通过用表达与由profilin基因的编码序列转录的mRNA互补的反义mRNA的基因转化植物或植物细胞并在植物或植物细胞中表达该基因来实现，使得反义mRNA 抑制植物细胞或植物细胞中profilin的产生。 植物中profilins的低表达可导致生长习性如矮矮化表型，根系和根系减少延迟以及开花发生的延迟。 通过用能够在植物或植物细胞中表达profilin的基因转化植物或植物细胞并使转化的基因在植物或植物细胞中表达，可以实现profilin的过表达。 植物中profilin的过度表达可导致生长习性如高表型，根系和根系发育扩张，叶表面积扩大和加速开花的发生。