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    DNA containing variant FRT sequences
    12.
    发明授权
    DNA containing variant FRT sequences 有权
    含有变体FRT序列的DNA

    公开(公告)号：US07476539B2

    公开(公告)日：2009-01-13

    申请号：US11267182

    申请日：2005-11-07

    申请人： Izumu Saito ,  Yumi Saito

    发明人： Izumu Saito ,  Yumi Saito

    IPC分类号： C12N5/10 ,  C07H21/04 ,  A61K31/711 ,  A01K67/00

    CPC分类号： A61K48/00 ,  A01K2217/05 ,  C12N15/85 ,  C12N2800/30

    摘要： To provide DNA comprising mutant FRT sequence which causes recombination reaction between two mutant FRT sequences having an identical sequence to each other but does not cause recombination reaction with a wild-type FRT sequence, in the presence of FLP recombinase; and a method for performing high-efficiency, gene insertion or gene replacement. A DNA comprising a mutant FRT sequence (any one of SEQ ID NOs: 2 to 5); a DNA comprising a mutant FRT sequence possessing (A) causing no specific DNA recombination reaction with wild type FRT, even if FLP recombinase is present, and (B) causing specific DNA recombination reaction with another mutant FRT sequence having an identical sequence thereto in the presence of recombinase FLP, wherein the mutant FRT sequence has substitutions of at least one nucleotide in a region other than the spacer region in the sequence and a cell which is transformed with the DNA.

    摘要翻译： 为了提供包含突变FRT序列的DNA，其导致两种具有相同序列的突变FRT序列之间的重组反应，但在FLP重组酶存在下不引起与野生型FRT序列的重组反应; 以及进行高效，基因插入或基因置换的方法。 包含突变FRT序列（SEQ ID NO：2至5中的任一个）的DNA; 即使存在FLP重组酶，也包含具有（A）不与野生型FRT进行特异性DNA重组反应的突变FRT序列的DNA，（B）在具有与其相同序列的另一个突变FRT序列中进行特异性DNA重组反应 存在重组酶FLP，其中突变FRT序列在序列中的间隔区以外的区域中具有至少一个核苷酸的取代，以及用DNA转化的细胞。

    Recombinase-expressing cells
    13.
    发明申请
    Recombinase-expressing cells 审中-公开
    表达重组蛋白酶的细胞

    公开(公告)号：US20060003443A1

    公开(公告)日：2006-01-05

    申请号：US11217321

    申请日：2005-09-02

    申请人： Izumu Saito ,  Yumi Kanegae

    发明人： Izumu Saito ,  Yumi Kanegae

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C12N5/00 ,  C12N5/02

    CPC分类号： C12N9/00 ,  C12N2799/022 ,  C12N2800/30

    摘要： In accordance with the present invention, there are provided cells for expressing recombinase Cre in the presence of recombinase FLP in a FLP-dependent manner, methods of expressing recombinase Cre by introducing recombinase FLP into the above cells, methods of producing recombinant viral vectors using cells that express recombinase Cre in the presence of recombinase FLP in a FLP-dependent manner, and methods of producing recombinant adenovirus vectors using the above method of producing Cre and the above method of producing recombinant viral vectors.

    摘要翻译： 根据本发明，提供了在重组酶FLP存在下以FLP依赖性方式表达重组酶Cre的细胞，通过将重组酶FLP引入上述细胞中表达重组酶Cre的方法，使用细胞产生重组病毒载体的方法 其以FLP依赖性方式在重组酶FLP存在下表达重组酶Cre，以及使用上述制备Cre的方法和上述生产重组病毒载体的方法生产重组腺病毒载体的方法。

    Recombinant adenovirus and process for producing the same
    15.
    发明授权
    Recombinant adenovirus and process for producing the same 失效
    重组腺病毒及其制备方法

    公开(公告)号：US5731172A

    公开(公告)日：1998-03-24

    申请号：US302312

    申请日：1994-09-08

    申请人： Izumu Saito ,  Yumi Kanegae

    发明人： Izumu Saito ,  Yumi Kanegae

    IPC分类号： C12N15/861 ,  C12N15/64 ,  C12N15/66

    CPC分类号： C12N15/86 ,  C12N2710/10343

    摘要： Disclosed are a recombinant adenovirus bearing a DNA sequence encoding a desired foreign polypeptide, and a hybrid promoter (CAG promoter) comprising a cytomegalovirus enhancer, a chicken .beta.-actin promoter, a rabbit .beta.-globin splicing acceptor and a polyA sequence, a process for production thereof, as well as use thereof for in genetic treatment. The recombinant adenovirus effectively expresses the foreign polypeptide in a wide range of animal cells.

    摘要翻译： 公开了具有编码期望的外来多肽的DNA序列的重组腺病毒和包含巨细胞病毒增强子，鸡β-肌动蛋白启动子，兔β-珠蛋白剪接受体和polyA序列的杂合启动子（CAG启动子） 其生产，以及其在遗传处理中的用途。 重组腺病毒在广泛的动物细胞中有效地表达外源多肽。

    Novel cosmid vector
    17.
    发明申请
    Novel cosmid vector 有权
    新型粘粒载体

    公开(公告)号：US20060172421A1

    公开(公告)日：2006-08-03

    申请号：US10553639

    申请日：2003-11-19

    申请人： Izumu Saito ,  Yumi Saito

    发明人： Izumu Saito ,  Yumi Saito

    IPC分类号： C12N15/861

    CPC分类号： C12N15/86 ,  C12N15/70 ,  C12N2710/10343

    摘要： A novel cosmid vector and the like effectively used in generating a recombinant adenoviral vector are provided. More specifically, there are provided a cosmid vector characterized by: (1) containing an adenoviral genome having adenoviral inverted terminal repeat sequences each having a complete nucleotide sequence, (2) having a deletion in an adenovirus E1 gene region, and (3) containing a restriction enzyme recognition sequence not present in the adenoviral genome, on both sides of the adenoviral genome; a method of generating a recombinant adenoviral vector using the cosmid vector; and a reagent for generating a recombinant adenoviral vector containing the cosmid vector as a component.

    摘要翻译： 提供了有效用于产生重组腺病毒载体的新型粘粒载体等。 更具体地，提供了一种粘粒载体，其特征在于：（1）含有腺病毒基因组，其具有各自具有完整核苷酸序列的腺病毒反向末端重复序列，（2）在腺病毒E1基因区域中具有缺失，和（3）含有 腺病毒基因组两侧不存在的限制酶识别序列; 使用粘粒载体产生重组腺病毒载体的方法; 以及用于产生含有粘粒载体作为组分的重组腺病毒载体的试剂。

    DNA containing variant FRT sequences
    18.
    发明申请
    DNA containing variant FRT sequences 有权
    含有变体FRT序列的DNA

    公开(公告)号：US20060094111A1

    公开(公告)日：2006-05-04

    申请号：US11267182

    申请日：2005-11-07

    申请人： Izumu Saito ,  Yumi Saito

    发明人： Izumu Saito ,  Yumi Saito

    IPC分类号： C12N15/861 ,  C12N15/87 ,  C12N5/06

    CPC分类号： A61K48/00 ,  A01K2217/05 ,  C12N15/85 ,  C12N2800/30

    摘要： To provide DNA comprising mutant FRT sequence which causes recombination reaction between two mutant FRT sequences having an identical sequence to each other but does not cause recombination reaction with a wild-type FRT sequence, in the presence of FLP recombinase; and a method for performing high-efficiency, gene insertion or gene replacement. A DNA comprising a mutant FRT sequence (any one of SEQ ID NOs: 2 to 5); a DNA comprising a mutant FRT sequence possessing (A) causing no specific DNA recombination reaction with wild type FRT, even if FLP recombinase is present, and (B) causing specific DNA recombination reaction with another mutant FRT sequence having an identical sequence thereto in the presence of recombinase FLP, wherein the mutant FRT sequence has substitutions of at least one nucleotide in a region other than the spacer region in the sequence; a cell which is transformed with the DNA; gene replacement method using the DNA in the presence of recombinase FLP; a transgenic animal carrying the DNA in a chromosome; a pharmaceutical comprising the DNA; and a specific DNA recombination method, characterized in that a specific DNA recombination reaction is carried out by using two mutant FRT sequences (SEQ ID NO: 32) in the presence of recombinase FLP.

    摘要翻译： 为了提供包含突变FRT序列的DNA，其导致两种具有相同序列的突变FRT序列之间的重组反应，但在FLP重组酶存在下不引起与野生型FRT序列的重组反应; 以及进行高效，基因插入或基因置换的方法。 包含突变FRT序列（SEQ ID NO：2至5中的任一个）的DNA; 即使存在FLP重组酶，也包含具有（A）不与野生型FRT进行特异性DNA重组反应的突变FRT序列的DNA，（B）在具有与其相同序列的另一个突变FRT序列中进行特异性DNA重组反应 重组酶FLP的存在，其中突变FRT序列在序列中的间隔区以外的区域中具有至少一个核苷酸的取代; 用DNA转化的细胞; 在重组酶FLP存在下使用DNA的基因置换方法; 在染色体中携带DNA的转基因动物; 包含DNA的药物; 和特异性DNA重组方法，其特征在于，在重组酶FLP存在下，通过使用两个突变FRT序列（SEQ ID NO：32）进行特异性DNA重组反应。

    Recombinant adenovirus
    19.
    发明授权
    Recombinant adenovirus 失效
    重组腺病毒

    公开(公告)号：US06740515B1

    公开(公告)日：2004-05-25

    申请号：US09856110

    申请日：2001-08-16

    申请人： Izumu Saito ,  Yumi Saito

    发明人： Izumu Saito ,  Yumi Saito

    IPC分类号： C12N1500

    CPC分类号： C12N15/86 ,  A61K48/00 ,  C12N2710/10343 ,  C12N2800/30

    摘要： Recombinant adenoviruses comprising the following sequences in the genome: (1) a left inverted terminal repeat: (2) a packaging signal: (3) a recombinase recognition sequence located at a region in between said left inverted terminal repeat and said packaging signal: and (4) at least one more recombinase recognition sequence which is located downstream of said packaging signal and which is recognized by the recombinase that recognizes the above recombinase recognition sequence are useful as a material for constructing highly safe vectors for gene therapy in the field of gene therapy.

    摘要翻译： 在基因组中包含以下序列的重组腺病毒：（1）左反向末端重复：（2）包装信号：（3）位于所述左反向末端重复和所述包装信号之间的区域的重组酶识别序列：和 （4）至少一个位于所述包装信号下游的重组酶识别序列，其被识别上述重组酶识别序列的重组酶识别，可用作构建基因治疗基因治疗基因的高度安全载体的材料 治疗。

    DNA containing variant FRT sequences
    20.
    发明授权
    DNA containing variant FRT sequences 有权
    含有变体FRT序列的DNA

    公开(公告)号：US07060499B1

    公开(公告)日：2006-06-13

    申请号：US10089380

    申请日：2000-09-28

    申请人： Izumu Saito ,  Yumi Saito

    发明人： Izumu Saito ,  Yumi Saito

    IPC分类号： C12N15/63 ,  C12N5/10 ,  C07H21/04 ,  A01K67/027 ,  A61K48/00

    CPC分类号： A61K48/00 ,  A01K2217/05 ,  C12N15/85 ,  C12N2800/30

    摘要： To provide DNA comprising mutant FRT sequence which causes recombination reaction between two mutant FRT sequences having an identical sequence to each other but does not cause recombination reaction with a wild-type FRT sequence, in the presence of FLP recombinase; and a method for performing high-efficiency, gene insertion or gene replacement. A DNA comprising a mutant FRT sequence. A DNA comprising a mutant FRT sequence possessing (A) causing no specific DNA recombination reaction with wild type FRT, even if FLP recombinase is present, and (B) causing specific DNA recombination reaction with another mutant FRT sequence having an identical sequence thereto in the presence of recombinase FLP; gene replacement method using the DNA in the presence of recombinase FLP; and a specific DNA recombination method, characterized in that a specific DNA recombination reaction is carried out by using two mutant FRT sequences in the presence of recombinase FLP.

    摘要翻译： 为了提供包含突变FRT序列的DNA，其导致两种具有相同序列的突变FRT序列之间的重组反应，但在FLP重组酶存在下不引起与野生型FRT序列的重组反应; 以及进行高效，基因插入或基因置换的方法。 包含突变FRT序列的DNA。 即使存在FLP重组酶，也包含具有（A）不与野生型FRT进行特异性DNA重组反应的突变FRT序列的DNA，（B）在具有相同序列的另一突变FRT序列引起特异性DNA重组反应 存在重组酶FLP; 在重组酶FLP存在下使用DNA的基因置换方法; 特异性DNA重组方法，其特征在于，通过在重组酶FLP存在下使用两个突变FRT序列进行特异性DNA重组反应。