公开(公告)号：US08216833B2

公开(公告)日：2012-07-10

申请号：US10553639

申请日：2003-11-19

申请人： Izumu Saito ,  Yumi Saito

发明人： Izumu Saito ,  Yumi Saito

IPC分类号： C12N15/861 ,  C12N15/70

CPC分类号： C12N15/86 ,  C12N15/70 ,  C12N2710/10343

摘要： A novel cosmid vector and the like effectively used in generating a recombinant adenoviral vector are provided. More specifically, there are provided a cosmid vector characterized by: (1) containing an adenoviral genome having adenoviral inverted terminal repeat sequences each having a complete nucleotide sequence, (2) having a deletion in an adenovirus E1 gene region, and (3) containing a restriction enzyme recognition sequence not present in the adenoviral genome, on both sides of the adenoviral genome; a method of generating a recombinant adenoviral vector using the cosmid vector; and a reagent for generating a recombinant adenoviral vector containing the cosmid vector as a component.

摘要翻译： 提供了有效用于产生重组腺病毒载体的新型粘粒载体等。 更具体地，提供了一种粘粒载体，其特征在于：（1）含有腺病毒基因组，其具有各自具有完整核苷酸序列的腺病毒反向末端重复序列，（2）在腺病毒E1基因区域中具有缺失，和（3）含有 腺病毒基因组两侧不存在的限制酶识别序列; 使用粘粒载体产生重组腺病毒载体的方法; 以及用于产生含有粘粒载体作为组分的重组腺病毒载体的试剂。

公开(公告)号：US07060499B1

公开(公告)日：2006-06-13

申请号：US10089380

申请日：2000-09-28

申请人： Izumu Saito ,  Yumi Saito

发明人： Izumu Saito ,  Yumi Saito

IPC分类号： C12N15/63 ,  C12N5/10 ,  C07H21/04 ,  A01K67/027 ,  A61K48/00

CPC分类号： A61K48/00 ,  A01K2217/05 ,  C12N15/85 ,  C12N2800/30

摘要： To provide DNA comprising mutant FRT sequence which causes recombination reaction between two mutant FRT sequences having an identical sequence to each other but does not cause recombination reaction with a wild-type FRT sequence, in the presence of FLP recombinase; and a method for performing high-efficiency, gene insertion or gene replacement. A DNA comprising a mutant FRT sequence. A DNA comprising a mutant FRT sequence possessing (A) causing no specific DNA recombination reaction with wild type FRT, even if FLP recombinase is present, and (B) causing specific DNA recombination reaction with another mutant FRT sequence having an identical sequence thereto in the presence of recombinase FLP; gene replacement method using the DNA in the presence of recombinase FLP; and a specific DNA recombination method, characterized in that a specific DNA recombination reaction is carried out by using two mutant FRT sequences in the presence of recombinase FLP.

摘要翻译： 为了提供包含突变FRT序列的DNA，其导致两种具有相同序列的突变FRT序列之间的重组反应，但在FLP重组酶存在下不引起与野生型FRT序列的重组反应; 以及进行高效，基因插入或基因置换的方法。 包含突变FRT序列的DNA。 即使存在FLP重组酶，也包含具有（A）不与野生型FRT进行特异性DNA重组反应的突变FRT序列的DNA，（B）在具有相同序列的另一突变FRT序列引起特异性DNA重组反应 存在重组酶FLP; 在重组酶FLP存在下使用DNA的基因置换方法; 特异性DNA重组方法，其特征在于，通过在重组酶FLP存在下使用两个突变FRT序列进行特异性DNA重组反应。

公开(公告)号：US07476539B2

公开(公告)日：2009-01-13

申请号：US11267182

申请日：2005-11-07

申请人： Izumu Saito ,  Yumi Saito

发明人： Izumu Saito ,  Yumi Saito

IPC分类号： C12N5/10 ,  C07H21/04 ,  A61K31/711 ,  A01K67/00

CPC分类号： A61K48/00 ,  A01K2217/05 ,  C12N15/85 ,  C12N2800/30

摘要： To provide DNA comprising mutant FRT sequence which causes recombination reaction between two mutant FRT sequences having an identical sequence to each other but does not cause recombination reaction with a wild-type FRT sequence, in the presence of FLP recombinase; and a method for performing high-efficiency, gene insertion or gene replacement. A DNA comprising a mutant FRT sequence (any one of SEQ ID NOs: 2 to 5); a DNA comprising a mutant FRT sequence possessing (A) causing no specific DNA recombination reaction with wild type FRT, even if FLP recombinase is present, and (B) causing specific DNA recombination reaction with another mutant FRT sequence having an identical sequence thereto in the presence of recombinase FLP, wherein the mutant FRT sequence has substitutions of at least one nucleotide in a region other than the spacer region in the sequence and a cell which is transformed with the DNA.

摘要翻译： 为了提供包含突变FRT序列的DNA，其导致两种具有相同序列的突变FRT序列之间的重组反应，但在FLP重组酶存在下不引起与野生型FRT序列的重组反应; 以及进行高效，基因插入或基因置换的方法。 包含突变FRT序列（SEQ ID NO：2至5中的任一个）的DNA; 即使存在FLP重组酶，也包含具有（A）不与野生型FRT进行特异性DNA重组反应的突变FRT序列的DNA，（B）在具有与其相同序列的另一个突变FRT序列中进行特异性DNA重组反应 存在重组酶FLP，其中突变FRT序列在序列中的间隔区以外的区域中具有至少一个核苷酸的取代，以及用DNA转化的细胞。

公开(公告)号：US20060172421A1

公开(公告)日：2006-08-03

申请号：US10553639

申请日：2003-11-19

申请人： Izumu Saito ,  Yumi Saito

发明人： Izumu Saito ,  Yumi Saito

IPC分类号： C12N15/861

CPC分类号： C12N15/86 ,  C12N15/70 ,  C12N2710/10343

摘要： A novel cosmid vector and the like effectively used in generating a recombinant adenoviral vector are provided. More specifically, there are provided a cosmid vector characterized by: (1) containing an adenoviral genome having adenoviral inverted terminal repeat sequences each having a complete nucleotide sequence, (2) having a deletion in an adenovirus E1 gene region, and (3) containing a restriction enzyme recognition sequence not present in the adenoviral genome, on both sides of the adenoviral genome; a method of generating a recombinant adenoviral vector using the cosmid vector; and a reagent for generating a recombinant adenoviral vector containing the cosmid vector as a component.

摘要翻译： 提供了有效用于产生重组腺病毒载体的新型粘粒载体等。 更具体地，提供了一种粘粒载体，其特征在于：（1）含有腺病毒基因组，其具有各自具有完整核苷酸序列的腺病毒反向末端重复序列，（2）在腺病毒E1基因区域中具有缺失，和（3）含有 腺病毒基因组两侧不存在的限制酶识别序列; 使用粘粒载体产生重组腺病毒载体的方法; 以及用于产生含有粘粒载体作为组分的重组腺病毒载体的试剂。

公开(公告)号：US20060094111A1

公开(公告)日：2006-05-04

申请号：US11267182

申请日：2005-11-07

申请人： Izumu Saito ,  Yumi Saito

发明人： Izumu Saito ,  Yumi Saito

IPC分类号： C12N15/861 ,  C12N15/87 ,  C12N5/06

CPC分类号： A61K48/00 ,  A01K2217/05 ,  C12N15/85 ,  C12N2800/30

摘要： To provide DNA comprising mutant FRT sequence which causes recombination reaction between two mutant FRT sequences having an identical sequence to each other but does not cause recombination reaction with a wild-type FRT sequence, in the presence of FLP recombinase; and a method for performing high-efficiency, gene insertion or gene replacement. A DNA comprising a mutant FRT sequence (any one of SEQ ID NOs: 2 to 5); a DNA comprising a mutant FRT sequence possessing (A) causing no specific DNA recombination reaction with wild type FRT, even if FLP recombinase is present, and (B) causing specific DNA recombination reaction with another mutant FRT sequence having an identical sequence thereto in the presence of recombinase FLP, wherein the mutant FRT sequence has substitutions of at least one nucleotide in a region other than the spacer region in the sequence; a cell which is transformed with the DNA; gene replacement method using the DNA in the presence of recombinase FLP; a transgenic animal carrying the DNA in a chromosome; a pharmaceutical comprising the DNA; and a specific DNA recombination method, characterized in that a specific DNA recombination reaction is carried out by using two mutant FRT sequences (SEQ ID NO: 32) in the presence of recombinase FLP.

摘要翻译： 为了提供包含突变FRT序列的DNA，其导致两种具有相同序列的突变FRT序列之间的重组反应，但在FLP重组酶存在下不引起与野生型FRT序列的重组反应; 以及进行高效，基因插入或基因置换的方法。 包含突变FRT序列（SEQ ID NO：2至5中的任一个）的DNA; 即使存在FLP重组酶，也包含具有（A）不与野生型FRT进行特异性DNA重组反应的突变FRT序列的DNA，（B）在具有与其相同序列的另一个突变FRT序列中进行特异性DNA重组反应 重组酶FLP的存在，其中突变FRT序列在序列中的间隔区以外的区域中具有至少一个核苷酸的取代; 用DNA转化的细胞; 在重组酶FLP存在下使用DNA的基因置换方法; 在染色体中携带DNA的转基因动物; 包含DNA的药物; 和特异性DNA重组方法，其特征在于，在重组酶FLP存在下，通过使用两个突变FRT序列（SEQ ID NO：32）进行特异性DNA重组反应。

公开(公告)号：US06740515B1

公开(公告)日：2004-05-25

申请号：US09856110

申请日：2001-08-16

申请人： Izumu Saito ,  Yumi Saito

发明人： Izumu Saito ,  Yumi Saito

IPC分类号： C12N1500

CPC分类号： C12N15/86 ,  A61K48/00 ,  C12N2710/10343 ,  C12N2800/30

摘要： Recombinant adenoviruses comprising the following sequences in the genome: (1) a left inverted terminal repeat: (2) a packaging signal: (3) a recombinase recognition sequence located at a region in between said left inverted terminal repeat and said packaging signal: and (4) at least one more recombinase recognition sequence which is located downstream of said packaging signal and which is recognized by the recombinase that recognizes the above recombinase recognition sequence are useful as a material for constructing highly safe vectors for gene therapy in the field of gene therapy.

摘要翻译： 在基因组中包含以下序列的重组腺病毒：（1）左反向末端重复：（2）包装信号：（3）位于所述左反向末端重复和所述包装信号之间的区域的重组酶识别序列：和 （4）至少一个位于所述包装信号下游的重组酶识别序列，其被识别上述重组酶识别序列的重组酶识别，可用作构建基因治疗基因治疗基因的高度安全载体的材料 治疗。

公开(公告)号：US06297659B1

公开(公告)日：2001-10-02

申请号：US09433208

申请日：1999-11-04

申请人： Yumi Saito

发明人： Yumi Saito

IPC分类号： G01R3126

CPC分类号： G01R27/14 ,  G01R31/2642 ,  G01R31/2858

摘要： In a test system, a plurality of semiconductor devices (samples) to be tested are connected in parallel. Resistors and switches are connected to the respective semiconductor devices in series. A voltage source (power supply) supplies a constant voltage for the respective semiconductor devices. An ammeter measures current that flows through the respective semiconductor devices. A voltmeter measures the respective voltage drops of the resistors. A controller detects the specific resistor that is changed in a resistance value when the current measured by the ammeter is reduced, and turns off the switch connected to the specific resistor.

摘要翻译： 在测试系统中，待测试的多个半导体器件（样品）并联连接。 电阻器和开关串联连接到相应的半导体器件。 电压源（电源）为各个半导体器件提供恒定电压。 电流表测量流过各个半导体器件的电流。 电压表测量电阻的各个电压降。 当电流表测量的电流减小时，控制器检测电阻值变化的特定电阻，并关闭与特定电阻相连的开关。

公开(公告)号：US5316006A

公开(公告)日：1994-05-31

申请号：US27537

申请日：1993-03-08

申请人： Katuyuki Inage ,  Yumi Saito ,  Zhang Zhimming ,  Toru Fujii ,  Osamu Shirasaki

发明人： Katuyuki Inage ,  Yumi Saito ,  Zhang Zhimming ,  Toru Fujii ,  Osamu Shirasaki

IPC分类号： A61B5/022 ,  A61B5/0225 ,  A61B7/04

CPC分类号： A61B7/045 ,  A61B5/0225

摘要： An electronic blood pressure meter including a cuff, a pressurizing means for inflating the cuff, a pressure sensing device for sensing a fluid pressure within the cuff, a pulse wave detecting device for detecting a pulse wave component included in the cuff, a systolic pressure estimating device for estimating a systolic pressure value based on the pulse wave and the cuff pressure detected in a pressurizing process, a pressurization volume computing device for computing a pressurization volume based on the estimated the systolic pressure value, a pressure decreasing device for decreasing the cuff pressure after reaching the pressurization volume, a blood vessel information detecting device for detecting blood vessel information such as pulse wave components in the pressure decreasing process, a blood pressure value determining device for determining systolic and diastolic pressure values based on the blood vessel information and the cuff pressure, a pulse wave component dispersion degree detecting device for detecting a dispersion degree of the pulse wave components obtained in the pressure decreasing process, and a pressurization volume controlling device for controlling the pressurization volume by the pressurizing device.

摘要翻译： 一种电子血压计，包括袖带，用于使袖带充气的加压装置，用于感测袖带内的流体压力的压力检测装置，用于检测包括在袖带中的脉搏波分量的脉搏波检测装置，收缩压估计 用于基于在加压过程中检测到的脉搏波和袖带压来估计收缩压值的装置，用于基于估计的收缩压值计算加压体积的加压容量计算装置，用于降低袖带压力的压力降低装置 在达到加压容积之后，用于检测血压信息的血管信息检测装置，例如压力降低过程中的脉波成分，血压值确定装置，用于根据血管信息和袖带确定收缩压和舒张压值 压力，脉波分量分散度 ee检测装置，用于检测在减压过程中获得的脉波成分的分散度;以及加压量控制装置，用于通过加压装置控制加压量。

公开(公告)号：US06388269B1

公开(公告)日：2002-05-14

申请号：US09535971

申请日：2000-03-27

申请人： Yumi Saito

发明人： Yumi Saito

IPC分类号： H01L2348

CPC分类号： H01L22/34 ,  H01L23/528 ,  H01L2924/0002 ,  H01L2924/00

摘要： The present invention provides a novel metal interconnection structure for evaluation on electromigration thereof, wherein a test metal interconnection to be evaluated on electromigration is connected through a plurality of bamboo-structured metal interconnections to a plug metal interconnection, and the bamboo-structured metal interconnection has a smaller sectioned area than the test metal interconnection while the plug metal interconnection has a larger sectioned area than the test metal interconnection.

摘要翻译： 本发明提供了一种用于电迁移的新型金属互连结构，其中通过多个竹结构金属互连将电迁移评估的测试金属互连连接到插头金属互连，并且竹结构金属互连具有 比测试金属互连更小的截面面积，而插头金属互连具有比测试金属互连更大的截面面积。

公开(公告)号：US20090026802A1

公开(公告)日：2009-01-29

申请号：US12052929

申请日：2008-03-21

申请人： Shinichi Imada ,  Manabu Abo ,  Hidetoshi Kurata ,  Norikazu Matsuura ,  Yumi Saito

发明人： Shinichi Imada ,  Manabu Abo ,  Hidetoshi Kurata ,  Norikazu Matsuura ,  Yumi Saito

IPC分类号： B62D21/15

CPC分类号： B62D21/152

摘要： A rear frame structure for a vehicle is disclosed, wherein an impact load can be satisfactorily absorbed in the case of a collision with a rear surface of the vehicle. The rear frame structure includes a pipe-shaped impact-absorbing member located between left and right rear frames. The impact-absorbing member has a center pipe that has a V shape in plan view, formed so as to protrude backward. The impact-absorbing member has left- and right-side pipes extending from the center pipe linearly upwardly to left and right end pipes. The impact load applied to the center pipe causes the left- and right-side pipes to undergo compressive deformation in their axial longitudinal directions as well as bending deformation towards the front of the vehicle body, whereby the impact load is absorbed.

摘要翻译： 公开了一种用于车辆的后框架结构，其中在与车辆的后表面碰撞的情况下可以令人满意地吸收冲击载荷。 后框架结构包括位于左右后框架之间的管状冲击吸收构件。 冲击吸收构件具有在俯视图中具有V形的中心管，其形成为向后突出。 冲击吸收构件具有从中心管线向上延伸到左右端管的左右管道。 施加到中心管的冲击载荷导致左侧管和右侧管在其轴向方向上经受压缩变形以及朝向车身前部的弯曲变形，从而吸收冲击载荷。