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公开(公告)号：US09017700B2
公开(公告)日：2015-04-28
申请号：US12594074
申请日：2008-03-31
IPC分类号： A61K39/002 , A01N1/02 , A61K35/62
CPC分类号： A01N1/02 , A01N1/0205 , A01N1/0215 , A61K35/62
摘要： The present application describes a novel, advantageous purification method which guarantees the embryonation capability and the viability of a worm egg suspension. The T. suis worm eggs are first treated in a non-embryonated condition in a sulfuric acid solution at about =ph 2. Bacteria and viruses are thereby killed successfully. The germination number of yeasts and fungi is reduced. In a second step, the pH-value is increased and an orally tolerated preservative is added. Yeasts and fungi are thereby killed successfully. The suspension medium used in the second purification step can then be used as a culture medium for additional production (embryonation) and storage.
摘要翻译： 本申请描述了一种新颖的有利的纯化方法，其保证蠕虫卵悬液的胚胎能力和活力。 首先在大约= ph 2的硫酸溶液中以非含胚胎状态处理猪蛔虫卵。因此细菌和病毒被成功杀死。 酵母和真菌的发芽数量减少。 在第二步中，增加pH值并加入口服耐受性防腐剂。 因此，酵母和真菌被成功杀死。 然后可以将用于第二纯化步骤的悬浮介质用作用于额外生产（胚胎化）和储存的培养基。
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12.发明授权公开(公告)号：US08993332B2
公开(公告)日：2015-03-31
申请号：US12993517
申请日：2009-05-20
申请人： Bernhard Tewes , Rudolf Wilhelm
发明人： Bernhard Tewes , Rudolf Wilhelm
IPC分类号： C12Q1/68 , G01N1/28 , G01N1/30 , G01N1/44 , G01N15/10 , G01N33/00 , G01N33/48 , G01N33/50 , C12Q1/04 , C12Q1/66
CPC分类号： C12Q1/6888 , C12Q1/04 , C12Q1/66 , C12Q2600/158 , G01N33/5029 , G01N2333/43526
摘要： A method for determining the biological activity of embryonated Trichuris eggs is described, in which at least one of the following determinations is carried out: a) Determination and/or confirmation of the stage of the embryonal development of helminth eggs with the aid of quantitative PCR analysis by using suitable marker sequences for ascertaining the copy number of the genomic DNA, b) Determination of the metabolic activity of embryonated helminth eggs by means of biochemical and/or molecular biological methods, c) Determination of the inducibility of gene expression in embryonated helminth eggs, d) Microscopic determination of the motility of helminth larvae in the egg over long periods of observation after pre-incubation at increased temperatures and/or e) Determination of the hatching rate of Trichuris larvae in a laboratory animal, wherein the intact embryonated eggs recovered from the contents of the intestine are quantified compared to an internal standard.
摘要翻译： 描述了用于确定含胚的Trichuris卵的生物活性的方法，其中进行以下测定中的至少一个：a）借助定量PCR测定和/或确认蠕虫卵的胚胎发育阶段 通过使用合适的标记序列来确定基因组DNA的拷贝数进行分析，b）通过生物化学和/或分子生物学方法确定含胚蠕虫卵的代谢活性，c）确定胚胎蠕虫中基因表达的诱导性 鸡蛋，d）在升高的温度下预孵育后长时间观察卵中蠕虫幼虫的运动性的微观测定和/或e）确定实验动物中Trichuris幼虫的孵化率，其中完整的胚胎蛋 与内标相比，从肠内容物中回收。
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13.发明申请Method For Characterising The Biological Activity Of Helminth Eggs, In Particular Trichuris Eggs 有权用于表征蠕虫卵的生物活性的方法，特别是三叶鸡蛋公开(公告)号：US20120060229A9
公开(公告)日：2012-03-08
申请号：US12993517
申请日：2009-05-20
申请人： Bernhard Tewes , Rudolf Wilhelm
发明人： Bernhard Tewes , Rudolf Wilhelm
CPC分类号： C12Q1/6888 , C12Q1/04 , C12Q1/66 , C12Q2600/158 , G01N33/5029 , G01N2333/43526
摘要： A method for determining the biological activity of embryonated Trichuris eggs is described, in which at least one of the following determinations is carried out: a) Determination and/or confirmation of the stage of the embryonal development of helminth eggs with the aid of quantitative PCR analysis by using suitable marker sequences for ascertaining the copy number of the genomic DNA, b) Determination of the metabolic activity of embryonated helminth eggs by means of biochemical and/or molecular biological methods, c) Determination of the inducibility of gene expression in embryonated helminth eggs, d) Microscopic determination of the motility of helminth larvae in the egg over long periods of observation after pre-incubation at increased temperatures and/or e) Determination of the hatching rate of Trichuris larvae in a laboratory animal, wherein the intact embryonated eggs recovered from the contents of the intestine are quantified compared to an internal standard.
摘要翻译： 描述了用于确定含胚的Trichuris卵的生物活性的方法，其中进行以下测定中的至少一个：a）借助定量PCR测定和/或确认蠕虫卵的胚胎发育阶段 通过使用合适的标记序列来确定基因组DNA的拷贝数进行分析，b）通过生物化学和/或分子生物学方法确定含胚蠕虫卵的代谢活性，c）确定胚胎蠕虫中基因表达的诱导性 鸡蛋，d）在升高的温度下预孵育后长时间观察卵中蠕虫幼虫的运动性的微观测定和/或e）确定实验动物中Trichuris幼虫的孵化率，其中完整的胚胎蛋 与内标相比，从肠内容物中回收。
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14.发明申请Method For Characterising The Biological Activity Of Helminth Eggs, In Particular Trichuris Eggs 有权用于表征蠕虫卵，特别是三叶鸡蛋的生物活性的方法公开(公告)号：US20110191869A1
公开(公告)日：2011-08-04
申请号：US12993517
申请日：2009-05-20
申请人： Bernhard Tewes , Rudolf Wilhelm
发明人： Bernhard Tewes , Rudolf Wilhelm
CPC分类号： C12Q1/6888 , C12Q1/04 , C12Q1/66 , C12Q2600/158 , G01N33/5029 , G01N2333/43526
摘要： A method for determining the biological activity of embryonated Trichuris eggs is described, in which at least one of the following determinations is carried out: a) Determination and/or confirmation of the stage of the embryonal development of helminth eggs with the aid of quantitative PCR analysis by using suitable marker sequences for ascertaining the copy number of the genomic DNA, b) Determination of the metabolic activity of embryonated helminth eggs by means of biochemical and/or molecular biological methods, c) Determination of the inducibility of gene expression in embryonated helminth eggs, d) Microscopic determination of the motility of helminth larvae in the egg over long periods of observation after pre-incubation at increased temperatures and/or e) Determination of the hatching rate of Trichuris larvae in a laboratory animal, wherein the intact embryonated eggs recovered from the contents of the intestine are quantified compared to an internal standard.
摘要翻译： 描述了一种用于确定含胚的Trichuris卵的生物活性的方法，其中进行以下测定中的至少一个：a）借助定量PCR测定和/或确认蠕虫卵的胚胎发育阶段 通过使用合适的标记序列来确定基因组DNA的拷贝数进行分析，b）通过生物化学和/或分子生物学方法确定含胚蠕虫卵的代谢活性，c）确定胚胎蠕虫中基因表达的诱导性 蛋，d）在升高的温度下预孵育后长时间观察卵中蠕虫幼虫的运动性的显微测定和/或e）测定实验动物中Trichuris幼虫的孵化率，其中完整的胚胎蛋 与内标相比，从肠内容物中回收。
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