公开(公告)号：US11655505B2

公开(公告)日：2023-05-23

申请号：US17172378

申请日：2021-02-10

Applicant: KABUSHIKI KAISHA TOSHIBA

Inventor： Keiko Ito ,  Mika Inada ,  Koji Hashimoto

IPC: C12Q1/6876 ,  C12P19/34 ,  C12N15/10 ,  C12Q1/6848

CPC classification number: C12Q1/6876 ,  C12N15/10 ,  C12P19/34 ,  C12Q1/6848 ,  C12Q2600/178 ,  C12Q1/6848 ,  C12Q2525/155 ,  C12Q2525/207 ,  C12Q2525/301 ,  C12Q2531/119

Abstract: According to one embodiment, a primer set for elongating a target short-chain nucleic acid containing a first sequence to obtain an elongated product is provided. The elongated product contains a second, a third, a fourth sequence, a complementary sequence of the 1′-th sequence and a sixth sequence. The complementary sequence of the 1′-th sequence is a loop primer sequence. The primer set contains a first elongation primer containing a first elongation primer sequence and a complementary sequence of the sixth sequence, and a second elongation primer containing a second elongation primer sequence, the fourth, the third, and the second sequence.

公开(公告)号：US11072822B2

公开(公告)日：2021-07-27

申请号：US15908044

申请日：2018-02-28

Applicant: KABUSHIKI KAISHA TOSHIBA

Inventor： Mika Inada ,  Koji Hashimoto ,  Keiko Ito

IPC: C12Q1/68 ,  C12Q1/6853

Abstract: In general, according to one embodiment, a method containing, reverse-transcribing the first sequence in the target RNA, to obtain a reverse-transcription product containing a first′ sequence complementary to the first sequence, dissociating the reverse-transcription product from the target RNA, hybridizing an elongation primer and the reverse-transcription product to elongate both, thereby obtain an elongation product, and maintaining the amplification reaction liquid containing the elongation product, a primer set and Tin(exo-) DNA polymerase and/or Bsm DNA polymerase under an amplification reaction condition, to amplify the first′ sequence.

公开(公告)号：US20140155289A1

公开(公告)日：2014-06-05

申请号：US14173310

申请日：2014-02-05

Applicant: KABUSHIKI KAISHA TOSHIBA

Inventor： Naoko NAKAMURA ,  Koji Hashimoto ,  Michie Hashimoto ,  Nobuhiro Gemma ,  Masaru Nikaido

IPC: C12Q1/68

CPC classification number: C12Q1/6837 ,  C12Q1/6853 ,  C12Q2525/301

Abstract: Embodiments relate to a method of analyzing nucleic acid. The nucleic acid analysis method includes subjecting a sample to an amplifying reaction using first and second primers, reacting an amplification product with a nucleic acid probe, measuring presence/absence of hybridization and/or a quantity thereof, and determining presence/absence of a target nucleic acid in the sample and/or a quantity of target nucleic acid. The amplification product obtained with the first or second primer forms a stem-and-loop structural body. With the detection of the presence/absence of the hybridization between a nucleic acid probe for the loop structure and the amplification product, and/or the amount thereof, the nucleic acid analysis is carried out on the sample.

Abstract translation: 实施方案涉及分析核酸的方法。 核酸分析方法包括使用第一和第二引物对扩增反应进行扩增反应，使扩增产物与核酸探针反应，测定杂交的存在/不存在和/或其量，并确定靶的存在/不存在 样品中的核酸和/或一定量的靶核酸。 用第一或第二底漆获得的扩增产物形成茎 - 环结构体。 通过检测环结构的核酸探针和扩增产物之间的杂交存在/不存在和/或其量，就对样品进行核酸分析。